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flower-shaped vector beam with double singularitie

Cognitive and discursive practices of identifi cation of Russians in the media

Статья поступила в редакцию 19.04.2017 г.Когнитивно-дискурсивные практики моделирования национально-гражданской идентичности россиян представлены в статье с двух позиций: с позиции адресанта и с позиции адресата медиатекста. В ходе анализа когнитивно-дискурсивных практик адресанта построена фреймово-слотовая модель доминантного текстового концепта «Русские» и представлено ее дискурсивное преобразование. Когнитивно-дискурсивные практики адресата описаны с помощью ряда социологических и психолингвистических методов.Cognitive and discourse modeling practices of national and civil identity of Russians are presented in the study from two perspectives: from the perspective of the author and from the perspective of the recipient of media text. Frame-slot model of the dominant text concept «Russian» was built during the analysis of the cognitive and discursive practices of the author and its discursive transformation presents. Cognitive and discursive practices of the recipient is described by sociological and psycho-linguistic methods of analysis.Исследование выполнено при финансовой поддержке РФФИ, проект «Национально-гражданская идентичность россиян в дискурсе СМИ: концепт “информационная война” как мобилизационный фактор идентификации» № 16-04-00460

Mechanical Chameleon through Dynamic Real-Time Plasmonic Tuning

The development of camouflage methods, often through a general resemblance to the background, has recently become a subject of intense research. However, an artificial, active camouflage that provides fast response to color change in the full-visible range for rapid background matching remains a daunting challenge. To this end, we report a method, based on the combination of bimetallic nanodot arrays and electrochemical bias, to allow for plasmonic modulation. Importantly, our approach permits real-time light manipulation readily matchable to the color setting in a given environment. We utilize this capability to fabricate a biomimetic mechanical chameleon and an active matrix display with dynamic color rendering covering almost the entire visible region

Micrographs of <i>Dinophysis miles</i> collected in this study.

<p>a) Side view of a 8-cell colony. b) Apical view of the 8-cell colony. c) Close-up view of two cells to show their attachment to each other at the end of the dorsal process, the visible nucleus (thick arrow), and the dark-stained plastid by Lugol's indicative of starch storage (thin arrow). d) A cell after Lugo's stain was removed, showing the anterior list (thick arrow), the sulcal list (thin arrow), and ribs (dashed arrow). e) Green fluorescence under blue light excitation of DNA stained with SYBR Green I in the nucleus (thick arrow) and plastid (thin arrow). f) Orange fluorescence from phycoerythrin in the plastids (arrow) under green excitation light. Scale bar = 50 µm in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029398#pone-0029398-g001" target="_blank">Fig. 1 A–F</a>.</p

Phylogenetic relationship of <i>D. miles</i> with other dinophysioid dinoflagellates inferred from ITS1-5.8S-ITS2.

<p>Sequence obtained in this study is bold-typed. Support of nodes is based on bootstrap values of ML/NJ with 1000 and 500 resamplings, respectively. Only values greater than 60 are shown. If only one of the two phylogenetic methods yielded significant support, the other is shown with “-”. <i>Prorocentrum micans</i> was used as the outgroup to root the tree. In this tree, <i>D. miles</i> appears as a distinct lineage, well separated from <i>D. tripos</i>, <i>D. norvegica</i>, <i>D. caudata</i>, and other <i>Dinophysis</i> species.</p

Phylogram of plastid SSU rDNA showing diverse types of plastids and symbionts in <i>D. miles</i>.

<p>Sequence obtained in this study is bold-typed. Support of nodes is based on bootstrap values of NJ/ML with 1000 and 500 resamplings, respectively. Only values greater than 60 are shown. If only one of the two phylogenetic methods yielded significant support, the other is shown with “-”. <i>Marinomonas</i> sp. was used as the outgroup to root the tree.</p

Additional file 2: of Ras enhances TGF-β signaling by decreasing cellular protein levels of its type II receptor negative regulator SPSB1

Figure S7. v-Ha-Ras N85A and v-Ha-Ras N86A increase the degradation rate of SPSB1. 293 T cells were co-transfected with indicated FLAG-SPSB1 and v-Ha-Ras/v-Ha-Ras mutant/pcDNA3 control vector for 24 h, then cells were treated with TGF-β (2 ng/ml). 36 h post-transfection, cells were exposed to cycloheximide (20 μg/ml) for indicated periods and lysed. Whole cell lysates were then examined for indicated proteins by immunoblotting (IB). Results are representative of experiments repeated at least once. Figure S8. TGF-β receptors levels regulate TGF-β signaling sensitivity and duration. MDCK cells were co-transfected with pCAGA-luc and indicated TβRII and/or TβRI and/or pcDNA3 control vector. 24 h later, cells were treated with ± TGF-β at indicated concentration for a further 24 h and lysed. Luciferase activity was determined as desribed in Fig. 6. Data are expressed as mean relative Smad3 luciferase activity (fold-induction) and error bars represent S.D. from representative experiments performed 3 times. * P < 0.05. Figure S9 & 10. Induced expression of SPSB1 suppresses TGF-β signaling in Ras transformed 21D1 cells through destabilizing TβRII. Doxycycline inducible FLAG-SPSB1 21D1 cells were cultured in ± doxycycline (2 μg/ml) for 2 (S.10) or 7 days (S.9). Whole cell lysates (S.9) were then examined for indicated proteins by immunoblotting (IB). Cells (S.10) were then transfected with pCAGA-luc. 24 h post-transfection, cells were treated with ± TGF-β (0.2 ng/ml) for a further 24 h and lysed. Luciferase activity was determined as desribed in Fig. 6. Data are expressed as mean relative Smad3 luciferase activity (fold-induction) and error bars represent S.D. from representative experiments performed 3 times. * P < 0.05. In all case, each experiment was repeated at least once, one representing result is showing. Figure S11 & 12. FLAG-SPSB1 and eGFP co-expression in the cells. 293 T cells (S.11) and 21D1 cells (S.12) were co-transfected with eGFP construct and FLAG-SPSB1/MYC-SPSB1 Y129A as indicated for 48 h. Fixed cells were stained with Hoechst dye. FLAG-SPSB1 was immunostained with mouse anti-FLAG antibody followed by Alexa546-conjugated secondary anti-mouse IgG. MYC-SPSB1 Y129A was immunostained with mouse anti-MYC antibody followed by Alexa546-conjugated secondary anti-mouse IgG. The expression of SPSB1/SPSB1 mutant (red) and eGFP was analyzed by fluorescent microscope (magnification = 20×) in 4 random fields. (PPT 3720 kb

Phylogenetic relationship of <i>D. miles</i> with other dinoflagellates inferred from <i>cox</i>1.

<p>Sequence obtained in this study is bold-typed. Support of nodes is based on bootstrap values of NJ/ML with 1000 and 500 resamplings, respectively. Only values greater than 60 are shown. If only one of the two phylogenetic methods yielded significant support, the other is shown with “-”. <i>Oxyrrhis marina</i> was used as the outgroup to root the tree. In this tree, <i>D. miles</i> is separated from <i>D. ovum</i> and <i>D. acuminata</i>.</p

Phylogenetic relationship of <i>D. miles</i> with other dinophysioid dinoflagellates inferred from LSU rDNA.

<p>Sequence obtained in this study is bold-typed. Support of nodes is based on bootstrap values of ML/NJ with 1000 and 500 resamplings, respectively. Only values greater than 60 are shown. If only one of the two phylogenetic methods yielded significant support, the other is shown with “-”. <i>Prorocentrum micans</i> was used as the outgroup to root the tree. In this tree, <i>D. miles</i> cannot be separated from <i>D. tripos</i> and <i>D. odiosa</i>.</p

Additional file 1: of Ras enhances TGF-β signaling by decreasing cellular protein levels of its type II receptor negative regulator SPSB1

Figure S1. EGF stimulation has no effect on SPSB1 protein degradation. 293 T cells were transfected with FLAG-SPSB1. 40 h post-transfection, cells were exposed to cycloheximide (20 μg/ml) for indicated periods with or without EGF (50 μg/ml, 5 mins pretreated) and lysed. Cell lysates were examined for indicated proteins by immunoblotting (IB). Results are representative of experiments repeated at least once. Figure S2. EGF stimulation does not alter the interaction between endogenouse Ras and SPSB1. 293 T cells were transfected with FLAG-SPSB1. 48 h post-transfection, indicated cells were stimulated with EGF (50 μg/ml) for 10 min and lysed. Thereafter, cell lysates were immunoprecipitated (IP) with anti-Ras antibody conjugated with protein G beads. Both whole cell lysates and immunoprecipatates were examined for indicated proteins by immunoblotting (IB). Results are representative of experiments repeated at least once. Figure S3–6. v-Ha-Ras N85A, v-Ha-Ras N86A and v-Ha-Ras D120A, R124A mutants do not disrupt their ability to interact with SPSB1. 293 T cells (S.3, 4, 5, 6) were transfected with indicated DNA constructs for 48 h. Thereafter, cell lysates were immunoprecipitated (IP) with anti-SPSB1 anibody (S.4) or anti-MYC antibody (S.5) or anti-Ras antibody (S.6) conjugated with protein G beads. Both whole cell lysates and immunoprecipatates were examined for indicated proteins by immunoblotting (IB). In all case, each experiment was repeated at least once, one representing result is shown. (PPT 3970 kb