Irrigation in Support of Research Project: Seismic Absorption and Modulus Measurement

The irrigation site located at the University of Arizona Maricopa Agricultural Center was designed to support controlled irrigation through the use of evenly spaced drip tubing under a heavy black plastic tarp. Soil solution samplers (lysimeters) are placed at specific locations to collect water from 1.5 meters, 3.0 meters and 10 meters from the soil surface. Suction provided by a vacuum pump was applied to the lysimeters for three hours prior to collection. After this time, the soil solution, if any was collected and placed into 20 ml HDPE scintillation vials and stored at -15 C for later analysis. These samples were analyzed for pH with a Ross Sure Flow Combination Electrode. Electrical conductivity for each sample was measured with the use of an Accumet Conductivity Cell. Both electrodes were connected to an Accumet Model 50 pH/ion/conductivity meter. High Performance Liquid Chromatography was employed in an attempt to determine the amount of Biosurfactant present in each sample. Our HPLC equipment includes a 5mm x 15mm CIS chromatography column, Waters 2487 detector, 510 pump, and WISP 710 Auto Injector. Millennium software was used to integrate data from these analyses

Azithromycin for sarcoidosis cough: an open label exploratory clinical trial

Background Chronic cough is a distressing symptom for many people with pulmonary sarcoidosis. Continuous treatment with a macrolide antibiotic may improve cough. We aimed to assess the potential efficacy of azithromycin in patients with sarcoidosis and self-reported cough.Methods We did a non-controlled, open label clinical trial of azithromycin 250 mg once daily for 3 months in patients with pulmonary sarcoidosis who reported a chronic cough. The primary outcome was number of coughs in 24 h. Secondary outcomes were cough visual analog scales and quality of life measured using the Leicester Cough Questionnaire and King's Sarcoidosis Questionnaire. Safety outcomes included QTc interval on ECG. Measurements were made at baseline and after one and 3 months of treatment.Results All 21 patients were white, median age 57 years, 9 males/12 females, median 3 years since diagnosis. Five were taking oral corticosteroids and none were taking other immunosuppressants. Twenty patients completed the trial. The median (range) number of coughs in 24 h was 228 (43–1950) at baseline, 122 (20–704) at 1 month, and 81 (16–414) at 3 months (p=0.002, Friedman's test). The median reduction in cough count at 3 months was 49.6%. There were improvements in all patient-reported outcomes. Azithromycin was well tolerated.Conclusion In a non-controlled open-label trial in people with sarcoidosis who reported a chronic cough, 3 months of treatment with azithromycin led to improvements in a range of cough metrics. Azithromycin should be tested as a treatment for sarcoidosis cough in a randomised placebo-controlled trial

Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions

During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic. Here we identify specific, essential meiotic functions for the Smc5/6 complex in homologous recombination and the regulation of cohesin. We show that Smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms, respectively. Smc5/6 also localizes to recombination hotspots, where it promotes normal formation and resolution of a subset of joint-molecule intermediates. In this regard, Smc5/6 functions independently of the major crossover pathway defined by the MutLγ complex. Furthermore, we show that Smc5/6 is required for stable chromosomal localization of the XPF-family endonuclease, Mus81-Mms4Eme1. Our data suggest that the Smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence, attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastroph

miR-199a-5p Is Upregulated during Fibrogenic Response to Tissue Injury and Mediates TGFbeta-Induced Lung Fibroblast Activation by Targeting Caveolin-1

As miRNAs are associated with normal cellular processes, deregulation of miRNAs is thought to play a causative role in many complex diseases. Nevertheless, the precise contribution of miRNAs in fibrotic lung diseases, especially the idiopathic form (IPF), remains poorly understood. Given the poor response rate of IPF patients to current therapy, new insights into the pathogenic mechanisms controlling lung fibroblasts activation, the key cell type driving the fibrogenic process, are essential to develop new therapeutic strategies for this devastating disease. To identify miRNAs with potential roles in lung fibrogenesis, we performed a genome-wide assessment of miRNA expression in lungs from two different mouse strains known for their distinct susceptibility to develop lung fibrosis after bleomycin exposure. This led to the identification of miR-199a-5p as the best miRNA candidate associated with bleomycin response. Importantly, miR-199a-5p pulmonary expression was also significantly increased in IPF patients (94 IPF versus 83 controls). In particular, levels of miR-199a-5p were selectively increased in myofibroblasts from injured mouse lungs and fibroblastic foci, a histologic feature associated with IPF. Therefore, miR-199a-5p profibrotic effects were further investigated in cultured lung fibroblasts: miR-199a-5p expression was induced upon TGFβ exposure, and ectopic expression of miR-199a-5p was sufficient to promote the pathogenic activation of pulmonary fibroblasts including proliferation, migration, invasion, and differentiation into myofibroblasts. In addition, we demonstrated that miR-199a-5p is a key effector of TGFβ signaling in lung fibroblasts by regulating CAV1, a critical mediator of pulmonary fibrosis. Remarkably, aberrant expression of miR-199a-5p was also found in unilateral ureteral obstruction mouse model of kidney fibrosis, as well as in both bile duct ligation and CCl4-induced mouse models of liver fibrosis, suggesting that dysregulation of miR-199a-5p represents a general mechanism contributing to the fibrotic process. MiR-199a-5p thus behaves as a major regulator of tissue fibrosis with therapeutic potency to treat fibroproliferative diseases. © 2013 Lino Cardenas et al

Cognition and bimanual performance in children with unilateral cerebral palsy: Protocol for a multicentre, cross-sectional study

© 2018 The Author(s). Background: Motor outcomes of children with unilateral cerebral palsy are clearly documented and well understood, yet few studies describe the cognitive functioning in this population, and the associations between the two is poorly understood. Using two hands together in daily life involves complex motor and cognitive processes. Impairment in either domain may contribute to difficulties with bimanual performance. Research is yet to derive whether, and how, cognition affects a child's ability to use their two hands to perform bimanual tasks. Methods/Design: This study will use a prospective, cross-sectional multi-centre observational design. Children (aged 6-12 years) with unilateral cerebral palsy will be recruited from one of five Australian treatment centres. We will examine associations between cognition, bimanual performance and brain neuropathology (lesion type and severity) in a sample of 131 children. The primary outcomes are: Motor - the Assisting Hand Assessment; Cognitive - Executive Function; and Brain - lesion location on structural MRI. Secondary data collected will include: Motor - Box and Blocks, ABILHAND- Kids, Sword Test; Cognitive - standard neuropsychological measures of intelligence. We will use generalized linear modelling and structural equation modelling techniques to investigate relationships between bimanual performance, executive function and brain lesion location. Discussion: This large multi-centre study will examine how cognition affects bimanual performance in children with unilateral cerebral palsy. First, it is anticipated that distinct relationships between bimanual performance and cognition (executive function) will be identified. Second, it is anticipated that interrelationships between bimanual performance and cognition will be associated with common underlying neuropathology. Findings have the potential to improve the specificity of existing upper limb interventions by providing more targeted treatments and influence the development of novel methods to improve both cognitive and motor outcomes in children with unilateral cerebral palsy

Late Season N Application Method Effect on Grain Protein, 2016

4 pp.Nitrogen fertilizer is normally applied later in the season around flowering time to boost grain protein content. The purpose of this study is to determine if the grain protein boost provided by late N application is affected by method of application. A trial testing late season N application methods was conducted at the Maricopa Ag Center in the 2016 growing season. The crop was grown 211 lb N/acre in split applications until flowering when 35 lb N/acre was applied as UAN32 in the irrigation water (fertigation), as low biuret urea in a foliar application, or as urea granules compared to no N application at all at flowering. In this study, we were not able to detect a difference in grain protein or any other variable measured due to the late N application method. We did measure a 0.4% increase in grain protein regardless of late season N application method compared to the control with no late N applied

Effects of High Electroconductivity Field Conditions on Production of Six Alfalfa Varieties on the Colorado River Indian Tribes Reservation

Five alfalfa varieties were planted and grown under grower conditions on a field section that had high conductivity, while two varieties were planted and grown on a section of the same field much less affected. CUF 101, the variety grown in both areas, was the highest yielding variety in both area. Reduction in CUF 101 hay yield due to high conductivity was approximately one ton/acre in the first year of production, with a large amount of this noted in the first cutting. Yield reductions of CUF 101 due to field area and associated electroconductivity were greater the second year, with an average of 0.29 tons/acre/cutting. Total yield difference for CUF 101 was 1.72 tons/acre for the eight harvests for which data were available. Yield differences between the areas was greatest in the early spring and late fall of the first harvest year, with differences not noted in the June cutting; in year two yield differences were approximately 37% for each cutting. Salado was the second highest yielding variety in the high electroconductivity area in 1998, and equaled CUF 101 in yield from this area in 1999. Sal-T-96 and Leivas Best yielded less than 90% of CUF 101. Sal-T-96 also had the greatest number of weeds, due in part to very slow germination and fewer plants per acre compared with other varieties

Evaluation of Palisade as a Plant Growth Regulator in Durum, 2016

3 pp.Lodging has historically been a problem in small grain production. Palisade is a relatively new plant growth regulator that has shown some promise in reducing lodging. The effect of Palisade on height and lodging of durum, and subsequent yield was tested in a study at the Maricopa Ag Center. Plant height was not affected by Palisade since the chemical was applied at boot, one stage past the recommended window where the plant was near maximum height. Lodging was reduced from 83 to 61% and 28 to 8% under the high and medium input growing conditions, respectively. Grain yield was increased from 4481 to 6152 lb/acre and 5600 to 7330 lb/acre under high and medium input growing conditions, respectively. Palisade is effective in reducing but not eliminating lodging, and can have a large impact on yield as in this study

First Year Effects of High Electroconductivity Field Conditions on Production of Six Alfalfa Varieties on the Colorado River Indian Tribes Reservation

Five alfalfa varieties were planted and grown under grower conditions on a field section that had high conductivity, while two varieties were planted and grown on a normal field area. CUF 101, the variety grown in both areas, was the highest yielding variety in both area. Reduction in CUF 101 hay yield due to high conductivity was approximately one ton/acre in the first year of production, with a large amount of this noted in the first cutting. Yield differences between the areas was greatest in the early spring and late fall, with differences not noted in the June cutting. Salado was the second highest yielding variety in the high electroconductivity area, with Sal-T-96 and Leivas Best yielding less than 90% of CUF 101. Sal-T-96 also had the greatest number of weeds, due in part to very slow germination and fewer plants per acre compared with other varieties