МикроРНК как важные диагностичеcкие предвестники развития акушерской патологии

MicroRNAs (miRs) are the class of short nucleotide sequences (21–27 nucleotides) RNA, non-coding protein synthesis. miRs are known as effective posttranscriptional negative regulators of gene expression with specific binding sites of targeted messenger RNA (mRNA) in the cytoplasm, providing translational repression or degradation of the target miR transcript. In this review we studied the role of miRNAs in the development of a physiological pregnancy and obstetric complications. The placenta is a unique organ which provides modulation of the immune system of the maternal organism during pregnancy including miRs which determine immunological tolerance of the body to the tissues of the fetus. Thus the «placental» miRs in maternal circulation may be the potential biomarker revealed at various obstetric pathology on the early stages before clinical manifestation of the diseases.МикроРНК (мкНК) — класс коротких нуклеотидных последовательностей (21–27 нуклеотидов) РНК, не участвующих в синтезе белка. мкРНК рассматривают как эффективные посттранскрипционные негативные регуляторы экспрессии генов, связывающи- еся со специфическими участками матричных РНК (мРНК) в цитоплазме, обеспечивающие репрессию трансляции или деградацию транскриптов мишеней мРНК. Показана роль мкРНК в развитии нормальной беременности и ее осложнений, уделено внимание профилям мкРНК на разных сроках физиологической беременности, особенно при иммунологической толерантности организма матери к тканям плода во время беременности. Также отмечены мкРНК, ассоциированные с иммуносупрессией, представлен профиль мкРНК, связанных с преэклампсией. Определение плацентарных мкРНК в материнском кровотоке рекомендовано исполь- зовать в качестве раннего прогностического биомаркера значимой акушерской патологии до наступления клинической манифестации заболевания

Biodynamic parameters of micellar diminazene in sheep erythrocytes and blood plasma

In this work, we used a preparation of diminazene, which belongs to the group of aromatic diamidines. This compound acts on the causative agents of blood protozoan diseases produced by both flagellated protozoa (Trypanosoma) and members of the class Piroplasmida (Babesia, Theileria, and Cytauxzoon) in various domestic and wild animals, and it is widely used in veterinary medicine. We examined the behavior of water-disperse diminazene (immobilized in Tween 80 micelles) at the cellular and organismal levels. We assessed the interaction of an aqueous and a water-disperse preparation with cells of the reticuloendothelial system. We compared the kinetic parameters of aqueous and water-disperse diminazene in sheep erythrocytes and plasma. The therapeutic properties of these two preparations were also compared. We found that the surface-active substances improved intracellular penetration of the active substance through interaction with the cell membrane. In sheep blood erythrocytes, micellar diminazene accumulated more than its aqueous analog. This form was also more effective therapeutically than the aqueous analog. Our findings demonstrate that use of micellar diminazene allows the injection dose to be reduced by 30%

Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2

Sustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of microplants of the cultivars Nevsky and Kondor with the strains Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2 separately and in combination. We examined the morphological variables of plant growth in in vitro culture and under ex vitro adaptation conditions; we also investigated the growth and performance of the plants in the greenhouse. The dependence of the inoculation eff icacy on potato genotype, growth stage, and inoculum composition was ascertained throughout the experiment. In vitro, A. baldaniorum Sp245 alone and in combination with O. cytisi IPA7.2 promoted the formation of roots on the microplants of both cultivars and the growth of Nevsky shoots. During plant growth ex vitro, all growth variables of the Nevsky microplants were promoted by O. cytisi IPA7.2 alone and in combination with A. baldaniorum Sp245. In both cultivars grown in the greenhouse, shoot growth was promoted in most inoculation treatments. The survival ability of the Nevsky microplants in the greenhouse increased 1.7-fold under the effect of simultaneous inoculation. Inoculation of microplants with a combination of A. baldaniorum Sp245 and O. cytisi IPA7.2 increased the number of Nevsky minitubers 1.5-fold and the number of Kondor minitubers 3.5-fold. Inoculation with the tested strains can be used to promote the growth of microplants and increase the yield of minitubers in potato seed breeding for the production of healthy planting material

Рефрактерный эпилептический статус после клипирования аневризмы средней мозговой артерии (клиническое наблюдение)

We present the management features for the refractory epileptic status in a patient after surgical treatment of unruptured cerebral aneurysm and no epileptic prehistory. The role of continuous electroencephalographic monitoring in adjusting the rate of drugs administration for general anesthesia in the treatment of this condition is also described.Приведена демонстрация и обсуждение особенностей ведения пациентки с рефрактерным эпилептическим статусом после хирургического лечения неразорвавшейся аневризмы головного мозга без эпилептического анамнеза. Показана роль непрерывного электроэнцефалографического мониторинга для подбора скорости введения препаратов для общей анестезии при лечении данного состояния

Проведение неинвазивной вспомогательной вентиляции легких в ходе санитарно-авиационной эвакуации у пациента с тяжелой внебольничной пневмонией

We report the experience of sanitary aviation evacuation of a patient with severe respiratory failure on the background of community-acquired pneumonia using mask non-invasive ventilation. The use of this method of ventilation of the lungs made it possible to avoid undesirable consequences arising from the transfer of the patient to artificial ventilation of the lungs and to transport him safely to a specialized medical institution in order to continue treatment. The described method of preparing a patient with respiratory failure before aviation transportation has shown its effectiveness during the flight and may be recommended for use by airmobile crews when carrying out long-distance evacuationПредставлен опыт санитарной авиационной эвакуации на дальнее расстояние пациента с тяжелой дыхательной недостаточностью на фоне внебольничной пневмонии с применением масочной неинвазивной вспомогательной вентиляции легких. Использование данного способа вентиляции легких позволило избежать нежелательных последствий, возникающих при переводе пациента на искусственную вентиляцию легких, и безопасно осуществить транспортировку в специализированное медицинское учреждение для продолжения лечения. Описываемый способ подготовки пациента с дыхательной недостаточностью перед авиационной транспортировкой показал свою эффективность во время полета и может быть рекомендован для использования аэромобильными бригадами при осуществлении эвакуации на дальние расстояния

MOLECULAR TAXONOMY AND THE EVOLUTION THEORY IN LIGHT OF EMERGING BIOINFORMATIC AND COSMOLOGICAL DATA

A brief review is given of recent advances in the taxonomic study of organisms and current views on biological evolution and the origin of life. A discussion is presented on how the treelike and net components contribute to the topology of phylogenetic constructs, with account taken of the prevailing role of horizontal gene transfer in prokaryote evolution and life. Approaches are described to the rational selection and practical use of phylogenetic markers (including 16S rRNA gene DNA sequences) in biomedical (including metagenomic) applications with traditional and nontraditional (large) amounts of molecular genetic data. Emerging results from taxonomic studies of the Earth’s biota and the methods of their generation are demonstrated. It is noted that the current developments in particle physics and in cosmology have important implications for solving paradoxes associated with the vanishingly small probability of some fundamental processes of prebiological and biological evolution