Difficulties of succession pipeline in large industrial enterprises of Nizhny Tagil in studies of tendencies of competitiveness of higher technical education

There have been significant changes in the higher education system in Russia recently. The possibility to apply for post-secondary education to several universities according to the results of the Unified State Examination (USE) and the transition to the two-level structure of higher education have significantly changed the landscape of competitiveness not only between individual universities, but also between large cities, which have become centers of mass attraction of applicants and young specialists. The number of students studying federally funded has been decreasing over the years. As a result, regional universities suffer a shortage of applicants, while local industrial enterprises suffer a shortage of young qualified personnel. The described problems are fully demonstrated in the system of higher education and training of young engineering personnel for large industrial enterprises in Nizhny Tagil. The article reviews the results of the study on the motivational field of academic migration of young people from the Nizhny Tagil city, conducted in February-March 2020. © Published under licence by IOP Publishing Ltd

A Theoretical and Experimental Study of Dipole Moments of 3-Aminofurazans

Dipole moments of a series of 3-amino-5-R-furazans (R = H, NH2, OCH3, CH3, N3, COOH, COOCH3, NO2) have been determined experimentally and also calculated by means of HF ab initio (STO-3G, 3-21G, 4-31G, 6-31G, 6-31G**/4-31G, 6-31G** levels) and semiempirical (MNDO, AM1, PM3) quantum chemical methods. It was shown that semiempirical AM1 and PM3 methods provide generally good agreement with the experimental values of dipole moments. On the other hand, a satisfactory description of this aminofurazan property by ab initio method is observed only in the case of calculation levels with the electron correlation and the polarization function included. For these compounds amino-imino tautomeric equilibrium is strongly shifted towards the amino-form. 3-Aminofurazan-4-carboxylic acid and its methyl ester exist in dioxane or benzene solutions at least as a mixture of two different s-cis- and s-trans-conformers stabilized by conjugation and hydrogen bonding

Basic methodical grounds of teaching international students chinese field-specific terms in technical institutes of higher education (in the context of polymer production)

We look at the problem connected with methods of teaching Chinese field-specific terms to international students in technical institutes of higher education. The basis of our research is represented by Chinese scientists’ articles in the sphere of manufacturing polymer. Our research is focused on the solution of problems connected with teaching Chinese field-specific terms to international students in technical institutes of higher education. The task required analysis of teaching and studying issues of field-specific disciplines in Tomsk Polytechnic University, study of terminological processes in Chinese, description of how field-specific terms are adopted from foreign languages into Chinese. Studying the above listed aspects provided a basis for developing a classification of polymer production terms. The way the Chinese language borrows a terminological item is considered when dividing terminological elements into groups for further systematization. We structured our classification of terms and terminological combinations by taking into account the fundamentals of high-molecular composition chemistry for easier understanding of polymer production terminology by teachers and students of technical institutions of higher education

HEAT TRANSMISSION CAPACITY IN THERMAL ISOLATION OF HEAT PIPE SYSTEMS

Экспериментально определены коэффициенты теплопроводности сухой и влажной изоляции трубопроводов тепловых сетей. Рассмотрена задача сушки влажной тепловой изоляции, проведено сопоставление с экспериментом.Heat transmission capacity coefficients for dry and moist isolation layers were experimentally obtained. The mathematical model was proposed to describe drying processes. Comparison has shown a good agreement between experimental data and calculation results

Functional magnetic ferrogels: From biosensors to regenerative medicine

Polyelectrolyte gels and ferrogels (FG) are attracting special interest in biomedicine. Here we describe our experience developing functional magnetic ferrogels for regenerative medicine and magnetoimpedance biosensing for measuring stray fields of nanoparticles in FG with a multilayered sensitive element. We discuss the possibility of developing a new generation of drug delivery systems for magnetic field assisted delivery, positioning and biosensing. © 2020 Author(s).This work was supported by the RSF project 18-19-00090. We thank O.M. Samatov, B. Stadler, V.N. Lepalovskij, M.A. Korch, A.V. Svalov, D.G. Blisnets, A. Larrañaga, I. Orue, and M.N. Volochaev for support. Selected measurements were made at SGIKER UPV/EHU

Protecting Mice from H7 Avian Influenza Virus by Immunisation with a Recombinant Adenovirus Encoding Influenza A Virus Conserved Antigens

Influenza is a highly contagious disease that causes annual epidemics and occasional pandemics. Birds are believed to be the source of newly emerging pandemic strains, including highly pathogenic avian influenza viruses of the subtype H7. The aim of the study: to evaluate the ability of the recombinant human adenovirus, serotype 5, which expresses genes of influenza A highly conserved antigens (ion channel M2 and nucleoprotein NP), to provide protection to laboratory mice against infection with a lethal dose of avian influenza virus, subtype H7. To achieve this goal, it was necessary to adapt influenza A virus, subtype H7 for reproduction in the lungs of mice, to characterise it, and to use it for evaluation of the protective properties of the recombinant adenovirus. Materials and methods: avian influenza virus A/Chicken/NJ/294508-12/2004 (H7N2) was adapted for reproduction in the lungs of mice by repeated passages. The adapted strain was sequenced and assessed using hemagglutination test, EID50 and LD50 for laboratory mice. BALB/c mice were immunised once with Ad5-tet-M2NP adenovirus intranasally, and 21 days after the immunisation they were infected with a lethal dose (5 LD50) of influenza virus A/Chicken/NJ/294508-12/2004 (H7N2) in order to assess the protective properties of the recombinant adenovirus. The level of viral shedding from the lungs of the infected mice was evaluated by titration of the lung homogenates in MDCK cell culture on days 3 and 6 after infection. The level of specific antibodies to H7 avian influenza virus was determined by indirect enzyme immunoassay. Results: the use of Ad5-tet-M2NP adenovirus for immunisation of the mice ensured 100% survival of the animals that had disease symptoms (weight loss) after their infection with the lethal dose (5 LD50) of H7 avian influenza virus. The study demonstrated a high post-vaccination level of humoral immune response to H7 avian influenza virus. The virus titer decreased significantly by day 6 in the lungs of mice that had been immunised with Ad5-tet-M2NP compared to the control group. Conclusion: the Ad5-tetM2NP recombinant adenovirus can be used to create a candidate pandemic influenza vaccine that would protect against avian influenza viruses, subtype H7, in particular

Способ получения рекомбинантных антител, продуцируемых клеточной линией, трансдуцированной рекомбинантными аденовирусами

Objectives. To develop a technology for obtaining recombinant antibodies in a suspension culture of human HEK293 cells using transduction with recombinant adenovirus serotype 5 (rAd5) carrying genes expressing heavy and light chains of antibodies on the example of two broadspectrum anti-influenza antibodies 27F3 and CR9114.Methods. Ad5-27F3-H, Ad5-CR9114-H, and Ad5-27F3-L recombinant adenoviruses carrying the 27F3 antibody heavy chain gene, CR9114 antibody heavy chain gene, and 27F3 light chain gene, respectively, were generated using the AdEasy™ Adenoviral vector system. To accumulate preparative amounts of recombinant r27F3 and rCR9114 antibodies, the HEK293 suspension cell line was transduced with recombinant adenoviruses carrying genes for heavy and light chains of antibodies. The cells were cultured in a wave-type bioreactor. Chromatography was used to purify recombinant antibodies from the culture medium. After analyzing the molecular weights of purified antibodies using protein electrophoresis, their ability to interact with influenza A and B viruses was analyzed using the Western blot technique, while their ability to neutralize influenza A and B viruses was evaluated using the virus neutralization assay.Results. A method for the accumulation and purification of recombinant r27F3 and CR9114 antibodies from the culture medium of a suspension culture of human cells following transduction with its recombinant adenoviruses carrying the genes for heavy and light chains of these antibodies was developed. The ability of the r27F3 antibody to interact with and neutralize influenza A viruses of group 1 (except influenza A virus subtype H2) and group 2 was shown. The ability of the rCR9114 antibody to interact with influenza A viruses of group 1 and influenza B viruses, as well as to neutralize influenza A viruses of group 1, was demonstrated.Conclusions. A technology for obtaining recombinant antibodies in a suspension culture of HEK293 cells using transduction with recombinant adenoviruses carrying genes expressing heavy and light chains of antibodies was developed along with a confirmation of their specificity.Цели. Разработать технологию получения рекомбинантных антител в суспензионной культуре клеток человека HEK293 с помощью трансдукции рекомбинантными аденовирусами человека пятого серотипа, несущими гены, экспрессирующие тяжелые и легкие цепи антител, на примере двух противогриппозных антител широкого спектра действия 27F3 и CR9114.Методы. Рекомбинантные аденовирусы Ad5-27F3-H, Ad5-CR9114-H и Ad5-27F3-L, несущие ген тяжелой цепи антитела 27F3, ген тяжелой цепи антитела CR9114 и ген легкой цепи 27F3, были получены с помощью набора AdEasy™ Adenoviral vector system. Для накопления препаративных количеств рекомбинантных антител r27F3 и rCR9114 суспензионную клеточную линию HEK293 трансдуцировали рекомбинантными аденовирусами, несущими гены тяжелых и легких цепей антител, и культивировали клетки в биореакторе волнового типа. Рекомбинантные антитела очищали из культуральной жидкости хроматографическим методом. Молекулярную массу полученных антител анализировали с помощью белкового электрофореза, их способность взаимодействовать с вирусами гриппа А и В методом вестерн-блот анализа, а способность нейтрализовать вирусы гриппа А и В с помощью реакции вирус-нейтрализации.Результаты. Отработана методика накопления и очистки рекомбинантных антител r27F3 и CR9114 из культуральной жидкости суспензионной культуры клеток человека после трансдукции ее рекомбинантными аденовирусами, несущими гены тяжелых и легких цепей этих антител. Показана способность антитела r27F3 взаимодействовать с вирусами гриппа А подгруппы 1 (кроме вируса грипп А субтипа H2) и подгруппы 2 и нейтрализовать их. Показана способность антитела rCR9114 взаимодействовать с вирусами гриппа А подгруппы 1 и вирусами гриппа В, а также нейтрализовать вирусы гриппа А подгруппы 1.Выводы. Отработана технология получения рекомбинантных антител в суспензионной культуре клеток HEK293 с помощью трансдукции рекомбинантными аденовирусами, несущими гены, экспрессирующие тяжелые и легкие цепи антител, и показана их специфичность

The Conditions for Selective Extraction of Precious Metal Ions by Thiocarbamoylated Polysilsesquioxane

Данная работа направлена на изучение сорбционных свойств впервые синтезированного поли(N-тиокарбамоил-3-(2-аминоэтил) аминопропилсилсесквиоксана) (ТКАЭАППСС) по отношению к ионам благородных металлов в условиях конкурентной сорбции. Исследование проводили в статическом и динамическом вариантах с использованием многокомпонентных растворов с различными значениями pH. Полученные результаты указывают на высокое сродство исследуемого полисилсесквиоксана к ионам Pt4+, Au3+, Ag+ и Pd2+ в многокомпонентных растворах, в отличие от ионов неблагородных металлов. В статических условиях наиболее полное извлечение Au3+ наблюдается при pH=2,5–7, Pt4+ – при pH=4, Pd2+ – при pH<4. Ag+ практически количественно извлекается во всем исследуемом интервале pH, что позволяет селективно извлечь данный ион из многокомпонентных систем. Ионы серебра (I) количественно сорбируются на ТКАЭАППСС в присутствии посторонних ионов при динамическом варианте сорбции, что в дальнейшем позволит разработать методики по селективному извлечению данного типа ионов из систем сложного состава.This work is devoted to the study of the sorption properties of the firstly synthesized the poly(N-thiocarbamoyl-3-(2-aminoethyl) aminopropylsilsesquioxane) with respect to precious metal ions in the conditions of competitive sorption. The study was carried out in static and dynamic versions using the multicomponent solutions with different pH values. The results obtained indicate a high affinity of the studied polysilsesquioxane for Pt4+, Au3+, Ag+, and Pd2+ ions in the multicomponent solutions, in contrast to base metal ions. In static conditions the most complete extraction of Au3+ is observed at pH = 2.5–7, Pt4+ at pH = 4, Pd2+ at pH <4. Ag+ is practically quantitatively extracted in the entire investigated pH range, which makes it possible to selectively separate this ion from the multicomponent systems. Silver (I) ions are quantitatively sorbed on TCAEAPPSS in the presence of foreign ions in the dynamic version of sorption, which in the future will allow the development of methods for the selective extraction of this type of ions from systems of complex composition

Экстренный экстра-интракраниальный микроанастомоз после каротидной эндартерэктомии, осложненной тромбозом внутренней сонной артерии

We report a case of emergency extra- intracranial microanastomosis in a patient with acute thrombosis of the internal carotid artery, formed on the first day after classical carotid endarterectomy using a temporary by-pass under conditions of contralateral occlusion. The causes of the complication are analyzed. A set of instrumental examinations was demonstrated, including multispiral computed tomography with angiography of the brain and extracranial arteries, as well as multispiral computed tomography with the study of parameters of cerebral perfusion after acetazolamide. The main stages of surgical correction, the dynamics of clinical indicators are presented. The conclusion was made about the effectiveness and safety of the chosen revascularization strategy.Представлен случай экстренного экстра-интракраниального микроанастомоза у пациента с острым тромбозом внутренней сонной артерии, сформировавшимся в первые сутки после классической каротидной эндартерэктомии с применением временного шунта в условиях контралатеральной окклюзии. Разобраны причины осложнения. Продемонстрирован комплекс инструментальных обследований, включающих мультиспиральную компьютерную томографию с ангиографией головного мозга и экстракраниальных артерий, а также мультиспиральную компьютерную томографию с изучением показателей перфузии головного мозга после приема ацетазоламида. Представлены основные этапы оперативной коррекции, динамика клинических показателей. Сделано заключение об эффективности и безопасности выбранной стратегии реваскуляризации

Мутации в геноме вирусов гриппа птиц подтипов Н1 и Н5, ответственные за адаптацию к млекопитающим

Avian influenza viruses of H1 and H5 subtypes were involved in the formation of highly pathogenic viruses that caused pandemics and panzootics in the 20th–21st centuries. In order to assess the zoonotic potential of viruses of these subtypes, two viruses of H1N1 and H5N3 have been isolated from wild ducks in Moscow and adapted to growth in mouse lungs. Their phenotypic properties were studied, and the genetic changes that occurred during adaptation were identified. The original A/duck/Moscow/4970/2013 (H1N1) and A/duck/Moscow/4182-C/2010 (H5N3) viruses were apathogenic for mice but became pathogenic after 7–10 passages in mouse lungs. Complete genome sequencing revealed 2 amino acid substitutions in the proteins of the H1N1 mouse-adapted variant (Glu627Lys in PB2 and Asp35Asn in hemagglutinin (HA) – numbering according to H3) and 6 mutations in the proteins of H5N3 virus (Glu627lys in PB2, Val113Ala in PB1, Ser82Pro in PB1-F2, Lys52Arg in HA2, Arg65Lys in NP, and Ser59Ile in NA). The increase in virulence is most likely due to a common substitution in the protein PB2 Glu627Lys as revealed in both viruses. The replacement of Asp35Asn in HA of the mouse-adapted H1N1 virus is associated with an increase in the pH value of the HA transition from 5.0 for 5.5 in comparison to the HA of parent virus. The found mutations in HA, NA, and PB1-F2 proteins of the adapted H5N3 variant are unique. The mutations Glu627Lys in PB2, Arg65Lys in NP, and Val113Ala in PB1 are most likely host adaptive.Вирусы гриппа птиц подтипов Н1 и Н5 участвовали в формировании высокопатогенных вариантов вирусов, вызвавших пандемии и  панзоотии в  XX–XXI  веках. С  целью оценки зоонозного потенциала вирусов этих подтипов, выделенных от диких уток в черте Москвы, была проведена адаптация вирусов к размножению в легких мышей, изучены их фенотипические свойства и идентифицированы генетические изменения, возникшие при адаптации. Изначально апатогенные для мышей вирусы A/duck/Moscow/4970/2013 (H1N1) и A/duck/Moscow/4182‑C/2010 (H5N3) после 7–10 пассажей через легкие мышей изменили фенотип на патогенный. Полногеномное секвенирование выявило в адаптированных к мышам вирусах 2 аминокислотные замены в вирусе гриппа H1N1 (Glu627Lys в белке PB2 и Asp35Asn в гемагглютинине (HA) — нумерация по H3) и 6 мутаций в белках вируса H5N3 (Glu627Lys в PB2, Val113Ala в PB1, Ser82Pro в PB1‑F2, Lys52Arg в HA2, Arg65Lys в NP и Ser59Ile в NA). Возрастание вирулентности для мышей, скорее всего, обусловлено общей для обоих вирусов заменой – Glu627Lys в  белке PB2. Замена Asp35Asn в  HA адаптированного к  мышам вируса гриппа H1N1  ассоциирована с возрастанием значения рН конформационного перехода HA с 5.0 до 5.5 относительно HA дикого вируса. Обнаруженные в адаптированном варианте H5N3 мутации в белках НА, NA и PB1‑F2 — уникальные. Мутации Glu627Lys в PB2, Arg65Lys в NP и Val113Ala в PB1, скорее всего, носят адаптационный характер