Characteristics of 146 treated HIV-infected children in Johannesburg, South Africa, at the time of measurement of cell-associated HIV-1 DNA (current time) and at the time of antiretroviral therapy (ART) initiation.

<p>Characteristics of 146 treated HIV-infected children in Johannesburg, South Africa, at the time of measurement of cell-associated HIV-1 DNA (current time) and at the time of antiretroviral therapy (ART) initiation.</p

Scatterplots showing associations between HIV-1 DNA log₁₀ copies/10⁶ cells and a: Time on ART in years; b: Pre-ART HIV-1 RNA copies/ml and c: Pre-ART CD4 count in cells/mm3.

<p>LOcally WEighted Scatter-plot Smoother (LOWESS) and linear regression prediction lines shown in blue and red, respectively.; grey area shows the 95% prediction elipse.</p

Comparison of <i>reverse transcriptase</i>-based single round qPCR (a and b) and sn-qPCR (c and d) assays designed to quantify subtype C HIV-1 proviral DNA.

<p>Amplification curves (a and c) of ten-fold diluted plasmid (p8MJ4) DNA harboring subtype C HIV-1 gag-pol gene and standard curves generated (b and d) from respective curves. Grey zone shows that the cycle threshold (C_T) region where 10⁵ plasmid DNA copies are amplified in the single round qPCR (a) is equivalent to the C_T region where a single copy (10°) is amplified using the sn-qPCR assay. Eff = PCR efficiency.</p

Number of specimens from EVD suspected cases in Sierra Leone tested daily (total blood and buccal swabs) by SA FEDL during the first weeks of operation, 25 August—30 Sept 2014.

<p>Column = Number of specimens tested daily; Dotted line = Trend line of a number of specimens tested; Solid line = Maximum testing capacity of 58 specimens per day.</p

Layout of the SA FEDL in Freetown-Lakka, Sierra Leone with emergency generator and wiring to allow for rapid switch to generator mode in case of power failure.

<p>(<b>A</b>) Biocontainment negative pressure chamber (IsoArk), (<b>B</b>) Room housing biocontainment negative pressure chamber, (<b>C</b>) Donning room, (<b>D</b>) Doffing room, (<b>E</b>) Laboratory airlock area, (<b>F</b>) PCR amplification room, (<b>G</b>) PCR master mix room, (<b>H</b>) Specimens and reagents storage area, (<b>I</b>) RNA extraction room, (<b>J</b>) Facility entrance, (<b>K</b>) Toilet, (<b>L</b>) Office 1, (<b>M</b>) Office 2, (<b>N</b>) Office 3. Petrol generator (5.5 kVa) placement indicated by the red rectangle, distribution of extension cords are indicated with red lines, and emergency connection points by red stars.</p

Results of EBOV L-gene RT-PCR in sera subjected to RNA manual (black dots) and automated (circles) extraction.

<p>Results of EBOV L-gene RT-PCR in sera subjected to RNA manual (black dots) and automated (circles) extraction.</p

Ebola virus polymerase gene TaqMan real-time RT-PCR results in blood specimens.

<p>Ebola virus polymerase gene TaqMan real-time RT-PCR results in blood specimens.</p

Operators dressed in BSL3 PPE (scrubs, Tyvek suits, surgical gown, double pair surgical gloves, gumboots, overshoes and PAPR with full face hood) entering the IsoArk main chamber through the airlock.

<p>Operators dressed in BSL3 PPE (scrubs, Tyvek suits, surgical gown, double pair surgical gloves, gumboots, overshoes and PAPR with full face hood) entering the IsoArk main chamber through the airlock.</p