Comparison of <i>reverse transcriptase</i>-based single round qPCR (a and b) and sn-qPCR (c and d) assays designed to quantify subtype C HIV-1 proviral DNA.
<p>Amplification curves (a and c) of ten-fold diluted plasmid (p8MJ4) DNA harboring subtype C HIV-1 gag-pol gene and standard curves generated (b and d) from respective curves. Grey zone shows that the cycle threshold (C<sub>T</sub>) region where 10<sup>5</sup> plasmid DNA copies are amplified in the single round qPCR (a) is equivalent to the C<sub>T</sub> region where a single copy (10°) is amplified using the sn-qPCR assay. Eff = PCR efficiency.</p
