Comparative study of antibacterial activity of two different earthworm species, Perionyx excavatus and Pheretima posthuma against pathogenic bacteria

Disease outbreaks are being increasingly recognized as a significant constraint on aquaculture production and trade affecting the economic development of the sector in many countries. Extracting and using biologically active compounds from earthworms has traditionally been practiced by indigenous people throughout the world. The aim of the present study was to shown antimicrobial activity through earthworm extract against fish bacterial pathogens. In total, 8 bacterial strains i.e. 6 gram negative viz. Aeromonas hydrophila, Pseudomonas aeruginosa, P. fluorescens, E.coli, Enterobacter aerogens and Shigella sp. and 2 gram positive viz. Staphylococcus aureus and Micrococcus luteus were identified. The extract of earthworm Perionyx excavatus, Pheretima posthuma were prepared and antimicrobial activity of the extract was determined by antimicrobial well diffusion assay. After 24 hrs of incubation period, it was observed that earthworm extract showed antibacterial activity against isolated bacterial strains. Among earthworm extract of two different species, the maximum zone of inhibition was shown against A. hydrophila by Perionyx excavatus (18.33± 0.66 mm) and P. posthuma (16.66±0.33). P. excavatus showed antibacterial activity against all pathogenic bacteria except Shigella spp. However on the other hand, P.posthuma showed antibacterial activity against A. hydrophila, P. fluorescens, E.coli, and S. aureus. The study has proved that earthworm extract can be effectively used for suppression of bacterial infection in fishes and that it can used as potential antimicrobial drug against commercial antibiotic resistance bacteria

The Rosiglitazone Controversy : The Indian Perspective

No Abstrac

Sensitivity Analysis of the Turning Motion of an Underwater Glider on the Viscous Hydrodynamic Coefficients

Autonomous underwater gliders (AUG) are a class of underwater vehicles that move using a buoyancy engine and forces from wings. Gliders execute turning motion with the help of a rudder or an internal roll control mechanism and the trajectory of the turn is a spiral. This paper analyses the sensitivity of the characteristics of spiral manoeuvre on the hydrodynamic coefficients of the glider. Based on the dynamics model of a gliding fish whose turn is enabled by a rudder, the effect of hydrodynamic coefficients of the hull and the rudder on the spiral motion are quantified. Local sensitivity analysis is undertaken using the indirect method. The order of importance of hydrodynamic coefficients is evaluated. It is observed that the spiral path parameters are most sensitive to the side force created by the rudder and the effect of the drag coefficient is predominant to that of the lift coefficients. This study will aid in quantifying the effect of change of geometry on the manoeuvrability of AUGs

Diarrhoeal disease outbreak in a rural area of Karnataka

Background: Acute diarrhoea is the passage of 3 or more loose or watery stools in the past 24 hours with or without dehydration. Owing to WASH strategy (Water, Sanitation and Hygiene) the burden of diarrheal diseases has seen a tremendous decline over the past 2 decades. Cholera is an acute diarrhoeal infection caused by ingestion of food or water contaminated with the bacterium Vibrio Cholerae. Objectives: 1. To document the factors responsible for the outbreak. 2. To provide recommendations for prevention and control of such outbreaks in future. Methods: After receiving verbal information from district office regarding outbreak of diarrhoeal disease (cholera) in a town of Bijapur district, we independently conducted a cross sectional study in the affected area and collected information regarding no. of people affected since the outbreak, their age and sex distribution. A total of 3802 people were interviewed using a predesigned questionnaire on 28th and 29th July, 2012. We also conducted environmental investigation regarding the source of contamination and collected 2 water samples from drinking water source. Results: All the cases were clustered in the five streets, which were consuming water from contaminated two water tanks. A total of 121 cases of diarrhoea were identified affecting 3.18% of the population. Attack rate of cholera was highest (4.5%) in 25-34 years age group followed by 4.22% in 15-24 years age group. Attack rates was higher among females (3.4%) compared to males (2.9%). Laboratory report stated that water samples were unfit for drinking purpose. V. Cholera (Ogawa serotype) was isolated from water sample. Conclusion: Consumption of contaminated water from a newly dug bore-well had led to the diarrhea outbreak. Lack of sanitation and hygiene had worsened the situation

Establishment of social clubs as a potential tool to educate adolescents living in urban field practice area of Shri BM Patil Medical college, Bijapur

Introduction: Globally half of the new HIV/AIDS cases are among the age group of 15 to 24 years and the greatest hope for changing the course of epidemic lies with the same agegroup. Within this age group, adolescents are more vulnerable to HIV infection as they do not have access to appropriate information or awareness of the dangers of high risk behavior. Objectives: Utilization and Impact of Social Clubs on imparting Knowledge to the Adolescents regarding HIV/ AIDS. Methodology: The study was conducted from Dec 2012 to Nov 2013. Assuming a 30 per cent prevalence of knowledge on HIV/AIDS, 95 per cent confidence interval and 15 per cent allowable error and 10 % dropout rate the calculated sample size was 438 adolescents Results: Out of 438 enrolled adolescents in the social club, 64 per cent participated in vocational training courses (62% girls and 38% boys) and also they participated in recreational activities and AIDS education sessions. The remaining (36%) participated only in recreational activities.88 per cent of adolescents attended more than 75 per cent of educational sessions held while 10 Per cent attended only 50 to 75 per cent of AIDS awareness sessions. Conclusion: The social clubs seem to have potentials to improve HIV/AIDS awareness among adolescents to adopt a healthy lifestyle and can be utilized to address various issues related to adolescents

Antiretroviral therapy does not reduce tuberculosis reactivation in a tuberculosis-HIV coinfection model

While the advent of combination antiretroviral therapy (ART) has significantly improved survival, tuberculosis (TB) remains the leading cause of death in the HIV-infected population. We used Mycobacterium tuberculosis/simian immunodeficiency virus-coinfected (M. tuberculosis/SIV-coinfected) macaques to model M. tuberculosis/HIV coinfection and study the impact of ART on TB reactivation due to HIV infection. Although ART significantly reduced viral loads and increased CD4+ T cell counts in blood and bronchoalveolar lavage (BAL) samples, it did not reduce the relative risk of SIV-induced TB reactivation in ART-treated macaques in the early phase of treatment. CD4+ T cells were poorly restored specifically in the lung interstitium, despite their significant restoration in the alveolar compartment of the lung as well as in the periphery. IDO1 induction in myeloid cells in the inducible bronchus-associated lymphoid tissue (iBALT) likely contributed to dysregulated T cell homing and impaired lung immunity. Thus, although ART was indispensable for controlling viral replication, restoring CD4+ T cells, and preventing opportunistic infection, it appeared inadequate in reversing the clinical signs of TB reactivation during the relatively short duration of ART administered in this study. This finding warrants the modeling of concurrent treatment of TB and HIV to potentially reduce the risk of reactivation of TB due to HIV to inform treatment strategies in patients with M. tuberculosis/HIV coinfection