Increasing Warm-Season Native Grass Biomass Using Fire, Herbicide, and Nitrogen Applications

The North American Great Plains tallgrass prairie was once a system of native cool and warm season grasses, which have been degraded by non-native invasive plants. Native grass restoration is highly desirable to improve ecosystem functions and productivity. In this two-year study, the impact of fire, herbicide, and nitrogen on productivity and the presence of invasive species [primarily the cool season grass, smooth brome (Bromus inermis Leyss.)] and native warm season native grass species [big bluestem (Andropogon gerardii Vitman), sideoats and blue grama (Bouteloua curtipendula (Michx.) Torr.), and B. gracilis (Willd. Ex Kunth) Lag. ex Griffiths] were investigated. Spring fire or a glyphosate application increased warm season grass biomass and decreased cool season grass biomass at peak warm season growth (August) during the treatment year. A second consecutive year of fire or herbicide further increased warm season grass biomass. If left untreated in the second year, cool season grasses tended to increase when sampled in August. Long-term management implementation is needed to suppress the tenacious cool season species and encourage the reestablishment of warm season grass populations

Critical examination of cohesive-zone models in the theory of dynamic fracture

We have examined a class of cohesive-zone models of dynamic mode-I fracture, looking both at steady-state crack propagation and its stability against out-of-plane perturbations. Our work is an extension of that of Ching, Langer, and Nakanishi (CLN) (Phys. Rev. E, vol. 53, no. 3, p. 2864 (1996)), who studied a non-dissipative version of this model and reported strong instability at all non-zero crack speeds. We have reformulated the CLN theory and have discovered, surprisingly, that their model is mathematically ill-posed. In an attempt to correct this difficulty and to construct models that might exhibit realistic behavior, we have extended the CLN analysis to include dissipative mechanisms within the cohesive zone. We have succeeded to some extent in finding mathematically well posed systems; and we even have found a class of models for which a transition from stability to instability may occur at a nonzero crack speed via a Hopf bifurcation at a finite wavelength of the applied perturbation. However, our general conclusion is that these cohesive-zone models are inherently unsatisfactory for use in dynamical studies. They are extremely difficult mathematically, and they seem to be highly sensitive to details that ought to be physically unimportant.Comment: 19 pages, REVTeX 3.1, epsf.sty, also available at http://itp.ucsb.edu/~lobkovs

Mob vs. Rotational Grazing: Impact on Forage Use and Artemisia absinthium

Short duration (≤24 h), high stocking density grazing systems (e.g., mob grazing) mimics historic prairie grazing patterns of American bison (Bison bison), and should minimize selective grazing. We compared mob [125 cow-calf pairs on either 0.65 ha for 12 h; or 1.3 ha for 24 h] vs. rotational [25 cow-calf pairs on 8.1 ha for 20 days starting in mid-May with or without 2,4-D application prior to grazing; and 15 days starting mid-April (no herbicide)] grazing systems based on forage utilization and impact to Artemisia absinthium (absinth wormwood) in a tall grass pasture of Eastern South Dakota. Grass height and density, and Artemisia absinthium patch volume were quantified pre- and post-grazing at sampling points along multiple transects. Mob grazing had >75% forage utilization, whereas rotational grazing averaged 50% (all consumption). Within a grazing season, three grazing systems suppressed Artemisia absinthium patches with rotation/spray (100% decrease) > mob (65 ± 10% decrease) > mid-May rotation (41 ± 16% decrease), whereas Artemisia absinthium patches in the mid-April rotation followed by summer rest dramatically increased in size. Artemisia absinthium patches <19,000 cm3 were browsed, whereas larger patches were trampled in mob-grazed areas, but avoided in rotational grazing. All Artemisia absinthium patches had regrowth the year following any grazing event

The effects of graded levels of calorie restriction : IV. Non-linear change in behavioural phenotype of mice in response to short-term calorie restriction

We would like to acknowledge the BSU staff for their invaluable help with caring for the animals. The work was supported by the UK Biotechnology and Biological Sciences Research Council BBSRC (BB/G009953/1 and BB/J020028/1).Peer reviewedPublisher PD

Soil and Land-Use Change Sustainability in the Northern Great Plains of the USA

In the Northern Great Plains (NGP), the combined impacts of land-use and climate variability have the potential to place many soils on the tipping point of sustainability. The objectives of this study were to assess if the conversion of grassland to croplands occurred on fragile landscapes in the North America Northern Great Plains. South Dakota and Nebraska were selected for this study because they are located in a climate transition zone. We visually classified 43,200 and 38,400 points in South Dakota and Nebraska, respectively, from high-resolution imagery in 2006, 2012, and 2014 into five different categories (cropland, grassland, habitat, NonAg, and water). The sustainability risk of the land-use changes was assessed based on the land capability class (LCC) scores at the selected sites. Sites with LCC scores ≤ 4 are considered sustainable for crop production if appropriate management practices are followed. Scores ≥ 6 are not considered suitable for row crop production. From 2006 to 2014, 910,000 and 360,000 ha of land were converted from grassland to cropland in South Dakota and Nebraska, respectively. Approximately 92 and 80% of the grassland conversion to croplands occurred on land suitable for crop production (land capability class, LCC ≤ 4) in South Dakota and Nebraska, respectively

A pilot study using metagenomic sequencing of the sputum microbiome suggests potential bacterial biomarkers for lung cancer

BBSRC (UK) support (BBS/E/W/10964A01A)Lung cancer (LC) is the most prevalent cancer worldwide, and responsible for over 1.3 million deaths each year. Currently, LC has a low five year survival rates relative to other cancers, and thus, novel methods to screen for and diagnose malignancies are necessary to improve patient outcomes. Here, we report on a pilot-sized study to evaluate the potential of the sputum microbiome as a source of non-invasive bacterial biomarkers for lung cancer status and stage. Spontaneous sputum samples were collected from ten patients referred with possible LC, of which four were eventually diagnosed with LC (LC+), and six had no LC after one year (LC-). Of the seven bacterial species found in all samples, Streptococcus viridans was significantly higher in LC+ samples. Seven further bacterial species were found only in LC-, and 16 were found only in samples from LC+. Additional taxonomic differences were identified in regards to significant fold changes between LC+ and LC-cases, with five species having significantly higher abundances in LC+, with Granulicatella adiacens showing the highest level of abundance change. Functional differences, evident through significant fold changes, included polyamine metabolism and iron siderophore receptors. G. adiacens abundance was correlated with six other bacterial species, namely Enterococcus sp. 130, Streptococcus intermedius, Escherichia coli, S. viridans, Acinetobacter junii, and Streptococcus sp. 6, in LC+ samples only, which could also be related to LC stage. Spontaneous sputum appears to be a viable source of bacterial biomarkers which may have utility as biomarkers for LC status and stagepublishersversionPeer reviewe

Spring Clipping, Fire, and Simulated Increased Atmospheric Nitrogen Deposition Effects on Tallgrass Prairie Vegetation

Defoliation aimed at introduced cool-season grasses, which uses similar resources of native grasses, could substantially reduce their competitiveness and improve the quality of the northern tallgrass prairie. The objective was to evaluate the use of early season clipping and fire in conjunction with simulated increased levels of atmospheric nitrogen deposition on foliar canopy cover of tallgrass prairie vegetation. This study was conducted from 2009 to 2012 at two locations in eastern South Dakota. Small plots arranged in a split-plot treatment design were randomized in four complete blocks on a warm-season grass interseeded and a native prairie site in east-central South Dakota. The whole plot consisted of seven treatments: annual clip, biennial clip, triennial clip, annual fire, biennial fire, triennial fire, and undefoliated control. The clip plots consisted of weekly clipping in May to simulate heavy grazing. Fire was applied in late April or early May. The subplot consisted of nitrogen applied at 0 or 15 kg N · ha−1 in early June. All treatments were initially applied in 2009. Biennial and triennial treatments were reapplied in 2011 and 2012, respectively. Canopy cover of species/major plant functional groups was estimated in late August/early September. Annual clipping was just as effective as annual fire in increasing native warm-season grass and decreasing introduced cool-season grass cover. Annual defoliation resulted in greater native warm-season grass cover, less introduced cool-season grass cover, and less native cool-season grass cover than biennial or triennial defoliation applications. Low levels of nitrogen did not affect native warm-season grass or introduced cool-season cover for any of the defoliation treatments, but it increased introduced cool-season grass cover in the undefoliated control at the native prairie site. This study supports the hypothesis that appropriately applied management results in consistent desired outcomes regardless of increased simulated atmospheric nitrogen depositions

Metagenomic Sequencing of the Chronic Obstructive Pulmonary Disease Upper Bronchial Tract Microbiome Reveals Functional Changes Associated with Disease Severity

Chronic Obstructive Pulmonary Disease (COPD) is a major source of mortality and morbidity worldwide. The microbiome associated with this disease may be an important component of the disease, though studies to date have been based on sequencing of the 16S rRNA gene, and have revealed unequivocal results. Here, we employed metagenomic sequencing of the upper bronchial tract (UBT) microbiome to allow for greater elucidation of its taxonomic composition, and revealing functional changes associated with the disease. The bacterial metagenomes within sputum samples from eight COPD patients and ten 'healthy' smokers (Controls) were sequenced, and suggested significant changes in the abundance of bacterial species, particularly within the Streptococcus genus. The functional capacity of the COPD UBT microbiome indicated an increased capacity for bacterial growth, which could be an important feature in bacterial-associated acute exacerbations. Regression analyses correlated COPD severity (FEV1% of predicted) with differences in the abundance of Streptococcus pneumoniae and functional classifications related to a reduced capacity for bacterial sialic acid metabolism. This study suggests that the COPD UBT microbiome could be used in patient risk stratification and in identifying novel monitoring and treatment methods, but study of a longitudinal cohort will be required to unequivocally relate these features of the microbiome with COPD severity