FROM MOUSTACHES TO MY SPACES

Radical removal of malignant lesions may be improved using tumor-targeted dual-modality probes that contain both a radiotracer and a fluorescent label to allow for enhanced intraoperative delineation of tumor resection margins. Because pretargeting strategies yield high signal-to-background ratios, we evaluated the feasibility of a pretargeting strategy for intraoperative imaging in prostate cancer using an anti-TROP-2 x anti-HSG bispecific antibody (TF12) in conjunction with the dual-labeled diHSG peptide (RDC018) equipped with both a DOTA chelate for radiolabeling purposes and a fluorophore (IRdye800CW) to allow near-infrared optical imaging. Nude mice implanted s.c. with TROP-2-expressing PC3 human prostate tumor cells or with PC3 metastases in the scapular and suprarenal region were injected i.v. with 1 mg of TF12 and, after 16 hours of tumor accumulation and blood clearance, were subsequently injected with 10 MBq, 0.2 nmol/mouse of either (111)In-RDC018 or (111)In-IMP288 as a control. Two hours after injection, both microSPECT/CT and fluorescence images were acquired, both before and after resection of the tumor nodules. After image acquisition, the biodistribution of (111)In-RDC018 and (111)In-IMP288 was determined and tumors were analyzed immunohistochemically. The biodistribution of the dual-label RDC018 showed specific accumulation in the TROP-2-expressing PC3 tumors (12.4 +/- 3.7% ID/g at 2 hours postinjection), comparable with (111)In-IMP288 (9.1 +/- 2.8% ID/g at 2 hours postinjection). MicroSPECT/CT and near-infrared fluorescence (NIRF) imaging confirmed this TROP-2-specific uptake of the dual-label (111)In-RDC018 in both the s.c. and metastatic growing tumor model. In addition, PC3 metastases could be visualized preoperatively with SPECT/CT and could subsequently be resected by image-guided surgery using intraoperative NIRF imaging, showing the preclinical feasibility of pretargeted dual-modality imaging approach in prostate cancer

Phylogeny of Geomydoecus and Thomomydoecus pocket gopher lice (phthiraptera, trichodectidae) inferred from cladistic analysis of adult and first instar morphology

The phylogeny for all 122 species and subspecies of chewing lice of the genera Geomydoecus and Thomomydoecus (Phthiraptera: Trichodectidae) hosted by pocket gophers (Rodentia: Geomyidae) is estimated by a cladistic analysis of fifty-eight morphological characters obtained from adults and first instars. The data set has considerable homoplasy, but still contains phylogenetic information. The phylogeny obtained is moderately resolved and, with some notable exceptions, supports the species complexes proposed by Hellenthal and Price over the the last two decades. The subgenera G. (Thaelerius) and T. (Thomomydoecus) are both shown to be monophyletic, but the monophly of subgenus T. (Jamespattonius) could not be confirmed, perhaps due to the lack of first-instar data for one of its component species. The nominate subgenus of Geomydoecus may be monophyletic, but our cladogram was insufficiently resolved to corroborate this. Mapping the pocket gopher hosts onto the phylogeny reveals a consistent pattern of louse clades being restricted to particular genera or subgenera of gophers, but the history of the host-parasite association appears complex and will require considerable effort to resolve

Al18F: A New Standard for Radiofluorination

Item does not contain fulltex

Placental composition and surface area but not vascularization are altered by maternal protein restriction in the rat

Amino acids are essential nutrients for foetal growth and development, and maternal protein restriction decreases foetal growth despite increasing placental size. To determine if placental structure is altered so as to impair function despite increased size, the effect of maternal protein restriction on the composition and vascularization of the placenta in the rat was determined. Pregnant rats were randomized to receive either a normal protein (20 per cent) or an isocaloric low (8 per cent) protein diet. Placentae were studied at day 18 of gestation. Tissue sections were stained with biotinylatedBandeiraea simplicifolia (BS-1) lectin to allow identification of foetal capillaries, and analysed using stereological techniques. Protein restriction increased total placental volume, but decreased the proportion of the placental volume composed of labyrinth. Consequently the total volumes of labyrinthine tissue and of foetal and maternal blood space were not different between the two groups. Protein restriction increased the surface area density and total surface area of the materno-foetal interface, but did not alter foetal capillary surface area, diameter or length. This study found that maternal protein restriction is associated with an increase in placental size, and that this enlargement is accompanied by increases in some, but not all, structural crelates of function

A study of two different creative processes and the challenge to find the right one for yourself

I mitt examensarbete jag har analyserat två olika skapandeprocesser, en konceptuellt grundad med fokus på hur skulptören Berlinde De Bruyckere arbetar och så den vi designstudenter lär oss under våra studier på Malmstens som har sin grund i Jan Landqvist bok ”Vilda idéer och djupodlande analys, Om designmetodikens grunder”.    Målet med denna analys har varit att hitta ett arbetssätt där jag finner trygghet och självförtroende som formgivare. Genom intervjuer med alumner från Malmstens möbeldesignprogram har jag fått förståelse för hur en som student kan arbeta efter examen. I framtagningen av mina möbler har jag haft min utgångspunkt i den moderna uppfattningen av rumsavdelning och avskärmning eller då kanske mer bristen på den. Då öppen planlösning och sociala ytor blir allt mer populärt i hemmen och där öppna kontorslandskap syns på var och varannat kontor har jag tagit fram två produkter som ställer sig frågande till just detta. Är det verkligen lika trevligt att leva utan väggar i vardagen som det är till fest? Finns det kanske en anledning till att vi ser fler och fler textila möbler och ”rum i rummet”-möbler på möbelmässan riktade mot öppna kontorslandskap för att skapa avskildhet? De möbler jag formgivit är en konceptuell rumsavdelare med utgång i ett konstnärligt arbetssätt. Rumsavdelaren är i form av en tratt som förstärker ljud, både önskade och oönskade. Här har jag jobbat i snabbt tempo och litat på min intuition inom god form. Min andra produkt är vad jag kallar ”En personlig sfär”. Här har jag använt mig av det arbetssätt jag lärt mig på Malmstens med informationsinsamling och skissetapper.  Ett till större det teoretiskt arbete där ens beslut ifrågasätts. Den ”personliga sfären” är tänkt att finnas i kontorsmiljö där man vill vara en del av rummet med inte bli störd av det som händer i ens direkta närhet.  Jag har genom mitt arbete kommit fram till att det arbetssätt som jag känner mig mest trygg med är en kombination av den process som lärs ut vid Malmstens och den mer fria, den konstnärligt grundade. Jag har också kommit fram till att den metod som lärs ut på skolan gett mig en grund utifrån vilken jag kan göra mina egna vägval.In my thesis, I have analyzed two different creative processes, the first one with a conceptual basis with a focus on how the sculptor Berlinde The Bruyckere work. The second one is based in design methods that we design students learn about during our studies at Malmsten that has its basis in Jan Landqvist book “Vilda idéer och djupodlande analys, Om designmetodikens grunder”.  The goal of this analysis was to find a way of working where I find safety and confidence as a designer. Through interviews with alumni from Malmstens furniture design program I have an understanding of how a student can work after graduation. In the development of my furniture, I’ve had my starting point of the modern perception of the room service and the foreclosure or then maybe more the lack of it. When the open-plan living and social spaces are becoming more popular in homes where open plan offices is visible on each every other office, I have developed two products that is concerned about just that. Is it really as nice to live without walls in everyday life as it is to the party? Is there perhaps a reason why we are seeing more and more textile and furniture “room within a room” commercial furniture at the furniture fair targeted to open offices to create privacy? The furniture I have designed is a conceptual room divider with the output of an artistic work. Room divider is in the form of a funnel that amplifies sound, both wanted and unwanted. Here I have been working in the fast paced and trusted my intuition in good shape. My second product is what I call “A personal sphere”. Here I have used the approach I learned at Malmsten with information gathering and sketching stages. One of the major theoretical work which even decisions questioned. The “personal sphere” is supposed to be in the office environment where you want to be a part of the room to not be bothered by what happens in one’s immediate vicinity. I have through my work come to the conclusion that the approach that I feel most comfortable with is a combination of the process that is taught at Malmsten and more free, conceptually founded. I have also come to the conclusion that the method taught at the school provided me with a basis on which I can make my own choices

Pretargeted immuno-PET of CEA-expressing intraperitoneal human colonic tumor xenografts: a new sensitive detection method.

Contains fulltext : 109142.pdf (publisher's version ) (Open Access)ABSTRACT: BACKGROUND: In this study, pretargeted immuno-positron-emission tomography [PET] with a bispecific monoclonal anti-carcinoembryonic antigen [CEA] (CEACAM5) x anti-hapten antibody (bispecific monoclonal antibody [bsmAb]) and a small (1.5 kD) peptide labeled with 68Ga was compared to fludeoxyglucose [18F-FDG]-PET for detecting intraperitoneal [i.p.] CEA-expressing human colonic tumor xenografts in nude mice. METHODS: Two groups of female BALB/c nude mice were inoculated with LS174T human colonic tumor cells i.p. One group received 5 MBq 18F-FDG, and the other received intravenous injections of the bsmAb, followed 16 h later with 5 MBq of 68Ga-labeled peptide. One hour after the radiolabeled peptide or FDG was given, micro-PET/computed tomography images were acquired. Thereafter, the uptake of the 68Ga or 18F in dissected tissue was determined. RESULTS: Within 1 h, high uptake of the 68Ga-labeled peptide in the tumor lesions (23.4 +/- 7.2% ID/g) and low background activity levels were observed (e.g., tumor-to-intestine ratio, 58 +/- 22). This resulted in a clear visualization of all intra-abdominal tumor lesions >/= 10 muL and even some tumors as small as 5 muL (2 mm diameter). 18F-FDG efficiently localized in the tumors (8.7 +/- 3.1% ID/g) but also showed physiological uptake in various normal tissues (e.g., tumor-to-intestine ratio, 3.9 +/- 1.1). CONCLUSIONS: Pretargeted immuno-PET with bsmAb and a 68Ga-labeled peptide could be a very sensitive imaging method for imaging colonic cancer, disclosing occult lesions

A simplified approach for F-18 radiolabeling of biomarker-binding peptides for PET imaging of cancer as part of a theranostic approach

Item does not contain fulltex