TNF-α inhibits glucose-induced insulin secretion in a pancreatic β-cell line (INS-1)

AbstractRecent studies suggest that TNF-α affects various biochemical and physiological processes which may be linked to the etiology of non-insulin-dependent diabetes mellitus (NIDDM). For example, TNF-α interferes with the signaling of the insulin receptor and the metabolism of glucose transporters. The possibility that TNF-α might directly reduce glucose-stimulated insulin secretion in pancreatic β-cells was examined by using an established pancreatic β-cell line (INS-1). TNF-α did not affect glucose-induced acute insulin secretion (30 min). However, over a longer time period (24 h), TNF-α decreased glucose-induced insulin secretion without affecting the total amount of insulin in the cell. In the presence of TNF-α levels of 0, 10, 100 and 1000 U/ml, the respective 20 mM glucose-induced insulin secretion was 1.736 ± 0.166, 1.750 ± 0.302, 1.550 ± 0.200, and 1.400 ± 0.112 mU/ml per 3 × 105 cells in 24 h

Regaining honour and regaining legitimacy: shame, obedience and risk practices amongst Chinese communist officials

As part of its anti-corruption campaigns in China, the Communist Party of China (CCP) provides officials opportunities to redeem themselves and renew their vows of loyalty to the Party and the people they serve. Officials must regain honour through a process of self-confrontation and self-renunciation in compulsory meetings in which they are encouraged to transform their immoral thoughts and behaviours through confessional criticism and self-criticism practices. These meetings facilitate officials’ redemption through a divinized, ritualistic and theatrical process. In the process of confession and penance, officials must expose themselves to a type of ritual martyrdom, which combines elements of shame, a commitment to absolute obedience and exposure to risk. This paper is based on original fieldwork comprising 50 interviews with high-, mid- and low-level officials across China during 2014 and 2015

Nurturing resilient future citizens through value consistency vs. the retreat from multiculturalism and securitisation in the promotion of British values in schools in the UK

The controversial duty to promote British values in Schools and Further Education Colleges in England, Scotland and Wales was introduced in the Counter-Terrorism and Security Act 2015. This duty is described by some as the introduction of securitising mechanism into British Schools, for others it is a symptom of the UK Government’s further retreat from Multiculturalism. In this article, we present the duty as the most recent reconstructive intervention designed to remedy alleged failings in the education system to produce liberal British citizens and to counter the growth of extremism. In the article, we will explore how local institutions such as schools and colleges have implemented the duty in a way that defuses some of the securitising aspects of the Statutory Duty through celebrating the UK as a multi-racial, multi-faith and multi-cultural society and through ensuring that their promotion of British values is consistent with their existing value system and ethos which includes the promotion of pupils’ Spiritual, Moral, Social and Cultural Development

Governing through 'the family' in China: Cultivating ethical political subjects through officials' 'nearest and dearest'

In this article we argue that the families of Communist Party members are increasingly being seen as both part of the problem and part of the solution to eradicating corruption in contemporary China. Our findings reveal how families are being investigated as well as co-opted by the party as a mechanism for encouraging its members to become ethical communist subjects. The current anti-corruption campaign in China is the context that has enabled this indirect governance of communist officials through the co-option of their ‘nearest and dearest’ in the party’s power structures. We argue that ‘the family’ in China is a privileged site for the remoralisation of society and the party through the process of facilitating what we call the ‘ethical subjectivities’ of officials. The contribution we make in this article is to analyse the continuum between the formal agencies of socialisation within the communist system and the informal but equally important institution of socialisation, namely, Communist Party members’ families

Formation stage and controlling factors of the paleo-uplifts in the Tarim Basin: A further discussion

AbstractVarious types of paleo-uplifts with different characteristics are developed in the Tarim Basin. Previously, there were multiple opinions on the pale-uplifts origins and structural evolution, so the oil and gas exploration ideas and deployment in the Tarim Basin were not developed smoothly. In this paper, regional seismic interpretation and structural analysis were carried out on the deep marine carbonate rocks in this basin based on the new seismic and drilling data. Then combined with the structural denudation results, the paleo-structural frameworks were reconstructed. And finally, the formation stage and main controlling factors of paleo-uplifts were discussed. It is shown that the Middle Ordovician is the key period when regional extension was converted to compression in this basin, so stratigraphic, sedimentary and structural differences occurred. Before the deposition of Yijianfang Fm in late Middle Ordovician, three carbonate paleo-uplifts (i.e., the Northern, Central and SW Tarim paleo-uplifts) begun to appear, and they were all broad-folded paleo-uplifts of nearly E–W striking and were formed at the same stage. The distribution and development of the Phanerozoic uplifts in this basin are restricted by the Northern and Southern Tarim basement pale-uplifts of nearly E–W striking which were developed during the Precambrian. It is indicated that all the three paleo-uplifts are compressional paleo-uplifts originated from the convergence of the southern plate margin based on the basement paleo-uplifts and they are all characterized by similar structural characteristics and inherited formation and evolution. The current differences of paleo-uplifts are controlled by multi-stage intense structural reformation since the Silurian. It is concluded that the oil and gas exploration potential is immense in the carbonate reservoirs of well-preserved deep paleo-structural zones in a larger area

Governing for and through harmonious community: The emergence of moral clinics in China

In this article, we advocate the adoption of ‘more temporal and processual characters’ to understand contemporary community governance in China. We show that communities in China are seen both as producing moral problems and as being the solutions to these problems. Furthermore, we argue that the establishment of the moral clinic provides an alternative to neoliberal ways of self-governance. In the article, we present moral clinics as a new form of community self-governance whose aim is to achieve a complex balance between various conflicts in the context of China’s unprecedented urbanisation in the name of governing for and through community harmony. Through examining the establishment of moral clinics, we expose how the relationship between the moral ‘hospitalisation’ of society and the socialisation of individuals can be understood in new ways. We argue that the institutionalisation of this ‘moral work’ is a strategy based on old techniques of Chinese traditional medicine that are being enhanced by modern organisational settings. In addition, we examine the micropolitics of the moral clinic through exposing the power relations behind its structural design, and especially its links with the state

Utilizing the moral nobility of older Chinese women in governance: The uses of humility, empathy, and an ethics of care in moral clinics in Huzhou city

This paper examines the emergence of the role of “moral doctors” who volunteer in what are called “moral clinics” in Huzhou city. In these moral clinics, the characteristics, experiences, and attributes of older women, in particular, are highly valued and viewed as being essential to the role of the moral doctor. These moral doctors act as moral exemplars and conflict mediators in their local communities. Their moral capital and professionalism, combined with their gender, age, familial and neighborhood attributes, contribute to the accumulation of an affective feminized labor which employs the techniques of care, reason, and moral fortitude to govern the self and others. We unpack these ethical virtues exemplified by moral doctors and nurses in order to show how a female-centric “ethic of care” can become a set of techniques in governing others. In this paper, we elaborate on the role that these moral doctors perform to support the aims of the moral clinics in terms of fostering pro-social behavior and moral obligation in local communities. We argue that the performance of this type of “moral work” is both a mechanism of discipline and a process of self-actualization. We contribute to the current literature on “therapeutic governance” in China by showing how the non-expert medicalization of social ills by moral doctors is incorporated into the reproduction of social control

Recovery of oil with unsaturated fatty acids and polyphenols from chaenomelessinensis (Thouin) Koehne: Process optimization of pilot-scale subcritical fluid assisted extraction

The potential effects of three modern extraction technologies (cold-pressing, microwaves and subcritical fluids) on the recovery of oil from Chaenomelessinensis (Thouin) Koehne seeds have been evaluated and compared to those of conventional chemical extraction methods (Soxhlet extraction). This oil contains unsaturated fatty acids and polyphenols. Subcritical fluid extraction (SbFE) provided the highest yield—25.79 g oil/100 g dry seeds—of the three methods. Moreover, the fatty acid composition in the oil samples was analysed using gas chromatography–mass spectrometry. This analysis showed that the percentages of monounsaturated (46.61%), and polyunsaturated fatty acids (42.14%), after applying SbFE were higher than those obtained by Soxhlet, cold-pressing or microwave-assisted extraction. In addition, the oil obtained under optimized SbFE conditions (35 min extraction at 35 °C with four extraction cycles), showed significant polyphenol (527.36 mg GAE/kg oil), and flavonoid (15.32 mg RE/kg oil), content, had a good appearance and was of high quality

Finding of widespread viral and bacterial revolution dsDNA translocation motors distinct from rotation motors by channel chirality and size

BACKGROUND: Double-stranded DNA translocation is ubiquitous in living systems. Cell mitosis, bacterial binary fission, DNA replication or repair, homologous recombination, Holliday junction resolution, viral genome packaging and cell entry all involve biomotor-driven dsDNA translocation. Previously, biomotors have been primarily classified into linear and rotational motors. We recently discovered a third class of dsDNA translocation motors in Phi29 utilizing revolution mechanism without rotation. Analogically, the Earth rotates around its own axis every 24 hours, but revolves around the Sun every 365 days. RESULTS: Single-channel DNA translocation conductance assay combined with structure inspections of motor channels on bacteriophages P22, SPP1, HK97, T7, T4, Phi29, and other dsDNA translocation motors such as bacterial FtsK and eukaryotic mimiviruses or vaccinia viruses showed that revolution motor is widespread. The force generation mechanism for revolution motors is elucidated. Revolution motors can be differentiated from rotation motors by their channel size and chirality. Crystal structure inspection revealed that revolution motors commonly exhibit channel diameters larger than 3 nm, while rotation motors that rotate around one of the two separated DNA strands feature a diameter smaller than 2 nm. Phi29 revolution motor translocated double- and tetra-stranded DNA that occupied 32% and 64% of the narrowest channel cross-section, respectively, evidencing that revolution motors exhibit channel diameters significantly wider than the dsDNA. Left-handed oriented channels found in revolution motors drive the right-handed dsDNA via anti-chiral interaction, while right-handed channels observed in rotation motors drive the right-handed dsDNA via parallel threads. Tethering both the motor and the dsDNA distal-end of the revolution motor does not block DNA packaging, indicating that no rotation is required for motors of dsDNA phages, while a small-angle left-handed twist of dsDNA that is aligned with the channel could occur due to the conformational change of the phage motor channels from a left-handed configuration for DNA entry to a right-handed configuration for DNA ejection for host cell infection. CONCLUSIONS: The revolution motor is widespread among biological systems, and can be distinguished from rotation motors by channel size and chirality. The revolution mechanism renders dsDNA void of coiling and torque during translocation of the lengthy helical chromosome, thus resulting in more efficient motor energy conversion