A virus-like particle of the hepatitis B virus preS antigen elicits robust neutralizing antibodies and T cell responses in mice

The preS antigen of hepatitis B virus (HBV) corresponds to the N-terminal polypeptide in the large (L) antigen in addition to the small (S) antigen. The virus-like particle (VLP) of the S antigen is widely used as a vaccine to protect the population from HBV infection. The presence of the S antigen and its antibodies in patient blood has been used as markers to monitor hepatitis B. However, there is very limited knowledge about the preS antigen. We generated a preS VLP that is formed by a chimeric protein between preS and hemagglutinin (HA), and the matrix protein M1 of influenza virus. The HBV preS antigen is displayed on the surface of preS VLP. Asn112 and Ser98 of preS in VLP were found to be glycosylated and O-glycosylation of Ser98 has not been reported previously. The preS VLP shows a significantly higher immunogenicity than recombinant preS, eliciting robust anti-preS neutralizing antibodies. In addition, preS VLP is also capable of stimulating preS-specific CD8+ and CD4+ T cell responses in Balb/c mice and HBV transgenic mice. Furthermore, preS VLP immunization provided protection against hydrodynamic transfection of HBV DNA in mice. The data clearly suggest that this novel preS VLP could elicit robust immune responses to the HBV antigen, and can be potentially developed into prophylactic and therapeutic vaccines

Mathematical Modeling of the Working Principle of Gravity Gradient Instrument

Gravity field is of great significance in geoscience, national economy and national security, and gravitational gradient measurement has been extensively studied due to its higher accuracy than gravity measurement. Gravity gradient sensor, being one of core devices of the gravity gradient instrument, plays a key role in measuring accuracy. Therefore, this paper starts from analyzing the working principle of the gravity gradient sensor by Newton's law, and then considers the relative motion between inertial and non-inertial systems to build a relatively adequate mathematical model, laying a foundation for the measurement error calibration, measurement accuracy improvement

Long-Term Stability of the SGA-WZ Strapdown Airborne Gravimeter

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Study on the impact of calculation radius on IDW gravity modelling

Gravity modelling is a necessary process of applying gravity data to practice, and a vital research area in resource exploring and geodesy. Inverse distance weighted (IDW) interpolation is a widely used algorithm in gravity modelling and many other griding procedures. Based on the gravity anomaly data acquired from the airborne gravimetry over a region in the Northwest of China, IDW method is analysed and implemented with varying calculation radius in gravity modelling. The results show that over the study area, with a proper calculation radius, the IDW can give a more precise prediction than quadric surfaces fitting (QSF) method. The distances between sampled points and unsampled points are the only information taken into consideration in the IDW interpolation, so the analysis of the influence by changing the calculation radius of the unsampled points in this test provides a new research direction of error eliminating when using IDW

STUDY ON SAFETY OF T PLATE CONTAINING CRACK BASED ON BS7910

Failure assessment diagram（ FAD） is usually used to evaluate the safety of welded structures containing cracks.There are three assessment levels in BS7910. As the assessment level increases,accuracy is improved and more data is required.This standard is widely used in safety assessment of pressure vessels and pipelines. The working level of offshore crane is relatively high and fatigue cracks can be found on it during the routine inspection. However,due to its high cost and long cycle of maintenance,the productivity is seriously affected. Therefore it is vital to commence safety evaluation on the cracked structure. This paper utilized Shao Yongbo’s experimental data to establish the 3C curves of the cracked structure of offshore crane,and compared it with the 2A curves of the BS7910 standard. The result shows that the tendencies of both curves are the same and 2A curve is more conservative. Thus the structure can be evaluated according to BS7910. This paper offers the critical load of T-plate that contains certain dimension of crack according to level 2A evaluation

An SINS/GNSS Ground Vehicle Gravimetry Test Based on SGA-WZ02

In March 2015, a ground vehicle gravimetry test was implemented in eastern Changsha to assess the repeatability and accuracy of ground vehicle SINS/GNSS gravimeter—SGA-WZ02. The gravity system developed by NUDT consisted of a Strapdown Inertial Navigation System (SINS), a Global Navigation Satellite System (GNSS) remote station on test vehicle, a GNSS static master station on the ground, and a data logging subsystem. A south-north profile of 35 km along the highway in eastern Changsha was chosen and four repeated available measure lines were obtained. The average speed of a vehicle is 40 km/h. To assess the external ground gravity disturbances, precise ground gravity data was built by CG-5 precise gravimeter as the reference. Under relative smooth conditions, internal accuracy among repeated lines shows an average agreement at the level of 1.86 mGal for half wavelengths about 1.1 km, and 1.22 mGal for 1.7 km. The root-mean-square (RMS) of difference between calculated gravity data and reference data is about 2.27 mGal/1.1 km, and 1.74 mGal/1.7 km. Not all of the noises caused by vehicle itself and experiments environments were eliminated in the primary results. By means of selecting reasonable filters and improving the GNSS observation conditions, further developments in ground vehicle gravimetry are promising

Compensation of Horizontal Gravity Disturbances for High Precision Inertial Navigation

Horizontal gravity disturbances are an important factor that affects the accuracy of inertial navigation systems in long-duration ship navigation. In this paper, from the perspective of the coordinate system and vector calculation, the effects of horizontal gravity disturbance on the initial alignment and navigation calculation are simultaneously analyzed. Horizontal gravity disturbances cause the navigation coordinate frame built in initial alignment to not be consistent with the navigation coordinate frame in which the navigation calculation is implemented. The mismatching of coordinate frame violates the vector calculation law, which will have an adverse effect on the precision of the inertial navigation system. To address this issue, two compensation methods suitable for two different navigation coordinate frames are proposed, one of the methods implements the compensation in velocity calculation, and the other does the compensation in attitude calculation. Finally, simulations and ship navigation experiments confirm the effectiveness of the proposed methods

Methods for Underwater Gravity Measurement Error Compensations Based on Correlation Analysis

The measurement of Earth’s gravitational field is important in geophysics, geodynamics, geodesy, oceanography, and space science. The ocean covers 71% of the earth’s surface; therefore, measuring the ocean’s gravitational field is crucial. Compared with shipborne gravimetry, underwater gravimetry near the seafloor is closer to gravity sources and can obtain short-wavelength gravity information that is useful for small-scale deposit detection and seawater intrusion monitoring. This article focuses on gravimetric errors caused by the poor dynamics of the carrier; an error compensation method for underwater gravimetry based on correlation analysis is proposed. By analyzing the error sources that affect the dynamics of the carrier, the relationship between the gravimetry error and impact factors related to the dynamics was established, and the model’s parameters were estimated by the least-squares fitting method. The experimental data show that this method can effectively compensate for gravimetric errors caused by carrier dynamics and provide the theoretical basis and algorithm model for underwater gravimetry in the bottom-tracking mode

Experimental Investigations on Airborne Gravimetry Based on Compressed Sensing

Gravity surveys are an important research topic in geophysics and geodynamics. This paper investigates a method for high accuracy large scale gravity anomaly data reconstruction. Based on the airborne gravimetry technology, a flight test was carried out in China with the strap-down airborne gravimeter (SGA-WZ) developed by the Laboratory of Inertial Technology of the National University of Defense Technology. Taking into account the sparsity of airborne gravimetry by the discrete Fourier transform (DFT), this paper proposes a method for gravity anomaly data reconstruction using the theory of compressed sensing (CS). The gravity anomaly data reconstruction is an ill-posed inverse problem, which can be transformed into a sparse optimization problem. This paper uses the zero-norm as the objective function and presents a greedy algorithm called Orthogonal Matching Pursuit (OMP) to solve the corresponding minimization problem. The test results have revealed that the compressed sampling rate is approximately 14%, the standard deviation of the reconstruction error by OMP is 0.03 mGal and the signal-to-noise ratio (SNR) is 56.48 dB. In contrast, the standard deviation of the reconstruction error by the existing nearest-interpolation method (NIPM) is 0.15 mGal and the SNR is 42.29 dB. These results have shown that the OMP algorithm can reconstruct the gravity anomaly data with higher accuracy and fewer measurements