Production and Optimization of Thermostable Alkaline Protease by Vibrio alginolyticus

Vibrio alginolyticus is a gram negative rod shaped bacterium which was enumerated in seawater from Chennai coast, Southeast coast of India. The isolates showed high thermostable alkaline protease activity which exhibited maximum at pH between 7.5 and 8.8 and maintains the stability at pH 8 at 36° C. The maximum activity of protease was found with 200 revolution per minute. The protease content was found to be 89% in V. alginolyticus. V. alginolyticus was resistance (95%) to Gentamycin and susceptible (100%) to Amikacin and Amoxyclav. The protease production did not show significant differences (P > 0.05) among concentrations of 15 µl, 20 µl and 25 µl in semi quantitative assay

Convex Optimization Algorithm for Product Recommendation Using Microblogging Information

The ecommerce and the social media is connected together where the products advertisements can be given by the ecommerce website which can be viewed by the social media users that is facebook users can  view the reviews given by the facebook friends or friends of friends of the users. The user may get an idea about the product features and also can be able to decide whether to buy the product or not. And also the user can give the review when the user knows about the product. In facebook database, all the reviews of the users or friends or friends of friends are stored. Among all the reviews, the overall count of the users who have given the positive comments and the negative comments are displayed .From this, the users can easily decide whether the product is useful or not. In the proposed system, convolutional neural network is used for more advanced learning

Comparison of pre- and post-ischemic treatment of telmisartan and nimodipine combination in experimentally induced cerebral ischemia

560-568Time dependent intervention plays a crucial role in preventing neurodegeneration after ischemic insult. The intensity of excitotoxicity is greater in the secondary reperfusion phase (2-4 h) compared to the primary occlusion phase (2 h), which could be attributed to secondary elevation of excitatory amino acids (EAA) in cerebral ischemia. In the present study, we tried to assess the neuroprotective effects of telmisartan and nimodipine (TM-NM) combination on the secondary reperfusion phase. The drug treatments were made immediately after reperfusion and their effects were compared with pre-treatment. The neuroprotective effect was studied using middle cerebral artery occlusion (MCAo) transient ischemic model in rats. On the 7th day after reperfusion, the rats were subjected to behavioral studies. The brain was dissected out on the 9th day to measure neurobiochemical alterations and for histopathological observations. The results have shown that TM-NM (5 mg/kg) attenuated the EAA release in different brain regions with partial restoration of energy levels in secondary reperfusion phase. Similarly, it normalized the behavioral alteration and the effect was comparable to pre-ischemic treatment (2.5 mg/kg). Pre-ischemic treatment of TM-NM (2.5 mg/kg) protected the neurons from ischemic reperfusion injury by energy dependent EAA regulation. It can be concluded from the study that, even though the pre- and post-treatment of TM-NM show similar results, the post-ischemic treatment of TM-NM combination is beneficial due to better EAA control. Since hypertension is the primary risk factor for stroke, clinical incidents of stroke in hypertensive patients receiving angiotensin receptor blockers (ARBs) can be further investigated to understand the present study in the clinical situation

Disseminated infection with <i>Strongyloides stercoralis</i> in a diabetic patient

A 58-year-old male diabetic who was operated for carcinoma larynx 4 years back was admitted with exertional dyspnoea and bilateral leg swelling for the past 2 years. Over the last 2 months, there was a progressive worsening of symptoms. Echocardiography done 2 years back showed pericardial effusion. Echo done during the current admission also showed pericardial effusion with preserved left ventricular function; cytological examination of the pericardial fluid showed larvae of Strongyloides stercoralis. He was treated with antinematodal drugs. A follow-up echo done at discharge showed no pericardial effusion and the patient was completely asymptomatic. To our knowledge, this is the first reported case of Strongyloides pericardial effusion in a diabetic patient

Elucidation of neuroprotective role of endogenous GABA and energy metabolites in middle cerebral artery occluded model in rats

391-397The excitatory amino acids (EAA) like glutamate, aspartate and inhibitory neurotransmitter GABA (gama amino butyric acid) play an important role in the pathophysiology of cerebral ischemia. The objective of the present study is to elucidate the role of endogenous GABA against EAA release in different regions during ischemia. The transient focal ischemia was induced in rats by using middle cerebral artery occlusion model (MCAo). The results indicate gradual elevation of brain glutamate, aspartate and GABA level at different brain regions and attained peak level at 72 h of ischemic reperfusion (IR). At 168 h of IR the EAA levels declined to base line but GABA level was found to be still elevated. The biochemical analysis shows the depleted brain ATP, Na+K+ATPase content and triphasic response of glutathione activity. It can be concluded that time dependent variation in the EAA and GABA release, endogenous GABA can be neuroprotective and earlier restoration of energy deprivation is essential to prevent further neurodegeneration. To have efficient treatment in ischemic condition, multiple approaches like energy supply, antagonism of EAA, controlling calcium function are essential.</span

Molybdenum oxide (MoO3) thin film based electrochromic cell characterisation in 0.1 M LiClO4.PC electrolyte

Electrochromic thin films of molybdenum oxide (MoO3) were prepared on transparent conducting oxide substrates, i.e. fluorine doped tin oxide coated (FTO or SnO2:F) glass substrates by electron beam evaporation technique using pure MoO3 (99?99%) pellets at various substrate temperatures (i.e. Tsub5room temperature (30uC), 100 and 200uC) under the vacuum of 161025 mbar. The room temperature prepared films were further annealed Tanne at 200 and 300uC for about one hour in the vacuum environment. The electrochemical nature of the films was studied by the cyclic voltammetry technique using a three electrode electrochemical cell in 0?1M LiClO4.PC electrolyte. The performance of the films was also tested by making electrochromic cells. The films produced at higher substrate temperature show lesser modulation in the visible spectrum, compared with the films produced at lower temperatures. A maximum colouration efficiency of 66 cm2 C21 was observed in the infrared region for the films prepared at room temperature