DETECTION OF RABIES VIRAL ANTIGEN IN CATTLE BY RAPID IMMUNOCHROMTOGRAPHIC DIAGNOSTIC TEST

In recent years, improved quality, accuracy and speed for diagnosis of rabies has been adopted for rabies control strategies in developing countries. In field condition, rapid immunochromtographic diagnostic test (RIDT) is a true requirement for rapid epidemiological surveillance of rabies. In the present study, a total of ten numbers of rabies suspected cattle brain sample form different parts of West Bengal, India were examined through RIDT. The results revealed that one sample was found to be positive. The test was established as powerful screening tool for rabies with high sensitivity and specificity. Thus, RIDT can be employed as a reliable and quick approach for diagnosis and control of rabies under field condition

PREVALENCE OF BOVINE HERPES VIRUS - 1 IN ORGANIZED FARMS OF WEST BENGAL, INDIA

Infectious Bovine Rhinotracheitis, caused by Bovine Herpesvirus-1 (BoHV-1) maintains latency in trigeminal nerve ganglia of bovine. The sero-positive bull infected with BoHV-1 secretes the virus through semen intermittently, when the immune system is compromised. Sera from bulls housed at different bull stations were analyzed using gE protein specific IDEXX Kit, which showed 78.69% positivity. Each batch of semen from sero-positive bull was investigated further for presence of virus in semen by Real Time-PCR technique for validation of presence of virus in the frozen semen doses using gB specific primers and probe, which showed 0.968 % semen batches positive. This study showed that despite high sero prevalence in bull, the semen excretes very negligible amount of the virus indicating the subtypes circulating in farms of West Bengal, India is assumed to be respiratory type

PREVALENCE OF BOVINE VIRAL DIARRHOEA VIRUS IN WEST BENGAL, INDIA

Bovine Viral Diarrhea (BVD) is one of the most economically important diseases in cattle. The present study was undertaken to diagnose the persistently infected (PI) animals by AntigenELISA and Reverse Transcriptase PCR using serum samples from organized farms as well as rural areas of West Bengal. The results showed that out of 964 serum samples tested 07 (0.73%) was positive for BVDV by Antigen-ELISA. For further confirmation, RNA was extracted from the positive samples and RT-PCR was performed with 5' UTR specific primers which showed 294 bp amplicons. This finding showed circulation of BVDV in cattle in West Bengal, India

DETECTION OF CLASSICAL SWINE FEVER VIRUS BY RT-PCR IN WEST BENGAL, INDIA

Classical swine fever is a deadly disease of swine, caused by a RNA virus. The present study has identified presence of the classical swine fever virus (CSFV) in pigs of West Bengal by one step reverse transcriptase PCR (RT-PCR) performed using 5’ NTR specific primers. Internal organs from clinically affected pigs were examined from three districts of West Bengal. RT-PCT has identified presence of CSFV in all the tissues examined confirming presence of CSFV in different parts of the state