Exploratory Animal and Medical Research, West Bengal Veterinary Alumni Association
Abstract
Bovine Viral Diarrhea (BVD) is one of the most economically important diseases in
cattle. The present study was undertaken to diagnose the persistently infected (PI) animals by AntigenELISA
and Reverse Transcriptase PCR using serum samples from organized farms as well as rural
areas of West Bengal. The results showed that out of 964 serum samples tested 07 (0.73%) was positive
for BVDV by Antigen-ELISA. For further confirmation, RNA was extracted from the positive samples
and RT-PCR was performed with 5' UTR specific primers which showed 294 bp amplicons. This
finding showed circulation of BVDV in cattle in West Bengal, India