The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice

Mature seeds of four upland rice cultivars namely Kusan, Lamsan, Selasi and Siam were assessed for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators, incorporated into MS (Murashige and Skoog) basal medium. Callus induction frequency was significantly different among the cultivars, as well as among the 2,4-Dichlorophenoxyacetic acid (2,4-D) levels tested. All tested cultivars exhibited highest callus frequency at 2 mgl-1 2,4-D. The incorporation of -naphthaleneacetic acid (NAA) and kinetin (Kin) in the callus induction medium supplemented with 2 mgl-1 2,4-D did not significantly improve the callus induction frequency. After two subcultures, at 24 days interval, the best response to callus induction was from cultivar Selasi, while callus browning became prominent in cultivars Kusan and Siam. Embryogenic callus placed on different regeneration media exhibited the highest regeneration frequency on medium containing 0.5 mgl-1 NAA + 2.0 mgl-1 Kin + 2.0 mgl-1 6-benzylaminopurine (BAP). The maximum regeneration frequency was achieved in cultivar Selasi followed by Lamsan while Siam and Kusan exhibited poor regeneration response. Among the four upland rice cultivars evaluated, Selasi and Lamsan are two promising cultivars in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus

Semantic Constraint and QoS-Aware Large-Scale Web Service Composition

Service-oriented architecture facilitates the running time of interactions by using business integration on the networks. Currently, web services are considered as the best option to provide Internet services. Due to an increasing number of Web users and the complexity of users’ queries, simple and atomic services are not able to meet the needs of users; and to provide complex services, it requires service composition. Web service composition as an effective approach to the integration of business institutions’ plans has taken significant acceleration. Nowadays, web services are created and updated in a moment. Therefore, in the real world, there are many services which may not have composability according to the conditions and constraints of the user's preferred choice. In the proposed method for automatic service composition, the main requirements of users including available inputs, expected outputs, quality of service, and the priority are initially and explicitly specified by the user and service composition is done with this information. In the proposed approach, due to a large number of services with the same functionality, at first, the candidate services are reduced by the quality of service-based Skyline method, and moreover, by using an algorithm based on graph search, all possible solutions will be produced. Finally, the user’s semantic constraints are applied on service composition, and the best composition is offered according to user’s requests. The result of this study shows that the proposed method is more scalable and efficient, and it offers a better solution by considering the user’s semantic constraints

Low molecular weight organic anions (carboxylates) increase microbial activity and alter microbial community composition in uncontaminated and diesel contaminated soil

Petroleum hydrocarbons (PHCs) are among the most prevalent sources of environmental contamination. It has been hypothesized that plant root exudation of low molecular weight organic acid anions (carboxylates) may aid degradation of PHCs by stimulating heterotrophic microbial activity. We, therefore, applied two commonly-exuded carboxylates (citrate and malonate) to uncontaminated and diesel contaminated microcosms (10,000 mg kg–1; aged 40 days) to determine their impact on the microbial community and PHC degradation. Every 48 hours for 18 days, soil received 5 μmol g–1 of i) citrate, ii) malonate, iii) citrate + malonate or iv) water. Microbial activity was measured daily as the flux of CO2. After 18 days, changes in the microbial community were assessed by community level physiological profiles and 16S rRNA bacterial community profiles determined by denaturing gradient gel electrophoresis. Saturated PHCs remaining in the soil were assessed by GCMS. Cumulative soil respiration increased four- to six-fold with the addition of carboxylates, while diesel contamination resulted in a small, but similar, increase across all carboxylate treatments. The addition of carboxylates resulted in distinct changes to the microbial community, but only a small decrease in the n-C17: pristane biomarker. We conclude that carboxylate addition can increase microbial activity and modify the microbial community in both uncontaminated and diesel-contaminated soils. The impact of these changes on PHC biodegradation and rhizosphere processes, more generally, merits further research

Citrate and malonate increase microbial activity and alter microbial community composition in uncontaminated and diesel-contaminated soil microcosms

Petroleum hydrocarbons (PHCs) are among the most prevalent sources of environmental contamination. It has been hypothesized that plant root exudation of low molecular weight organic acid anions (carboxylates) may aid degradation of PHCs by stimulating heterotrophic microbial activity. To test their potential implication for bioremediation, we applied two commonly exuded carboxylates (citrate and malonate) to uncontaminated and diesel-contaminated microcosms (10 000 mg kg−1; aged 40 days) and determined their impact on the microbial community and PHC degradation. Every 48 h for 18 days, soil received 5 µmol g−1 of (i) citrate, (ii) malonate, (iii) citrate + malonate or (iv) water. Microbial activity was measured daily as the flux of CO2. After 18 days, changes in the microbial community were assessed by a community-level physiological profile (CLPP) and 16S rRNA bacterial community profiles determined by denaturing gradient gel electrophoresis (DGGE). Saturated PHCs remaining in the soil were assessed by gas chromatography–mass spectrometry (GC-MS). Cumulative soil respiration increased 4- to 6-fold with the addition of carboxylates, while diesel contamination resulted in a small, but similar, increase across all carboxylate treatments. The addition of carboxylates resulted in distinct changes to the microbial community in both contaminated and uncontaminated soils but only a small increase in the biodegradation of saturated PHCs as measured by the n-C17 : pristane biomarker. We conclude that while the addition of citrate and malonate had little direct effect on the biodegradation of saturated hydrocarbons present in diesel, their effect on the microbial community leads us to suggest further studies using a variety of soils and organic acids, and linked to in situ studies of plants, to investigate the role of carboxylates in microbial community dynamics

Distribution of staphylococcal cassette chromosome mec types among methicillin-resistant coagulase negative staphylococci in central Iran

Background and Objectives: Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are important nosocomial pathogens. They may serve as a reservoir of SCCmec, the genomic island encoding amongst other methicillin resistance. This study was designed to determine the distribution of different SCCmec types from MR-CoNS isolated from clinical specimens in a tertiary hospital in central Iran, having high frequency of nosocomial methicillin-resistant staphylococcal infections. Materials and Methods: We evaluated isolates from patients attending the Vali-Asr Hospital located in the center of Iran, from February to December 2012. Multiplex PCR was performed for SCCmec typing. For isolates in which SCCmec could not be typed directly, additional ccr and mec complex analyses were performed. Results: Totally, 70 MR-CoNS isolates, comprising of 47 S. epidermidis strains (67%), 10 S. saprophyticus (14.3%), 9 S. hemolyticus (13%) and 4 S. lugdunensis (5.7%) were identified. Thirty-nine were characterized as type IVa 19 (27%), type III 11 (16%), type II 7 (10%) and type V 2 (3%). Only 20 isolates (28.6%) carried the ccr complex, while the current methods could not characterize the 11 remaining isolates. Conclusion: A high level of SCCmec genetic diversity was found among MR-CoNS isolates. MR-CoNS may act as a reservoir of SCCmec IV for MRSA. This issue should be taken into consideration seriously

Layer-by-layer functionalized nanotube arrays: A versatile microfluidic platform for biodetection

We demonstrate the layer-by-layer (LbL) assembly of polyelectrolyte multilayers (PEM) on three-dimensional nanofiber scaffolds. High porosity (99%) aligned carbon nanotube (CNT) arrays are photolithographically patterned into elements that act as textured scaffolds for the creation of functionally coated (nano)porous materials. Nanometer-scale bilayers of poly(allylamine hydrochloride)/poly(styrene sulfonate) (PAH/SPS) are formed conformally on the individual nanotubes by repeated deposition from aqueous solution in microfluidic channels. Computational and experimental results show that the LbL deposition is dominated by the diffusive transport of the polymeric constituents, and we use this understanding to demonstrate spatial tailoring on the patterned nanoporous elements. A proof-of-principle application, microfluidic bioparticle capture using N-hydroxysuccinimide-biotin binding for the isolation of prostate-specific antigen (PSA), is demonstrated.National Science Foundation (U.S.) (Award DMR-0819762

Densification of the interlayer spacing governs the nanomechanical properties of calcium-silicate-hydrate

Calciuam-silicate-hydrate (C-S-H) is the principal binding phase in modern concrete. Molecular simulations imply that its nanoscale stiffness is 'defect-driven', i.e., dominated by crystallographic defects such as bridging site vacancies in its silicate chains. However, experimental validation of this result is difficult due to the hierarchically porous nature of C-S-H down to nanometers. Here, we integrate high pressure X-ray diffraction and atomistic simulations to correlate the anisotropic deformation of nanocrystalline C-S-H to its atomic-scale structure, which is changed by varying the Ca-to-Si molar ratio. Contrary to the 'defect-driven' hypothesis, we clearly observe stiffening of C-S-H with increasing Ca/Si in the range 0.8 ≤ Ca/Si ≤ 1.3, despite increasing numbers of vacancies in its silicate chains. The deformation of these chains along the b-axis occurs mainly through tilting of the Si-O-Si dihedral angle rather than shortening of the Si-O bond, and consequently there is no correlation between the incompressibilities of the a- and b-axes and the Ca/Si. On the contrary, the intrinsic stiffness of C-S-H solid is inversely correlated with the thickness of its interlayer space. This work provides direct experimental evidence to conduct more realistic modelling of C-S-H-based cementitious material