Evaluation of efficient method for acclimatization of an important ornamental rhizomatic plant, Calathea crotalifera

Calathea crotalifera is one of the important exotic ornamental plants belonging to Marantaceae family. This rhizomatic species can produce a very attractive inflorescence and have been widely used in the horticulture field including landscape and cut flower industry. It usually propagated through cutting rhizome and showed slow growth rate. This study was conducted to evaluate the effects of different propagation substrate (mix soil medium and organic medium) and shade levels (10%, 60% and 90%) on growth, morphogenesis, and physiological development of micropropagated C. crotalifera plantlets after two months acclimatized in the natural environment. Transplantation survival and growth rate of plantlets is higher (90%) in an organic medium compared to mix soil medium (75%). It is observed that plantlets grown under 60% shade level produced healthy seedlings with higher plants height, larger specific leaf area, leaf diameter, leaf length, leaf thickness and dry mass. The concentration of chlorophyll a and b, and carotenoid is also higher in this treatment. There are no significant effects on the new shoot production among the treatments. Micromorphological of the leaf surface were evaluated using light and scanning electron microscope. The stomatal frequency per unit leaf area in the adaxial leaf surface decreased significantly (p< 0.05) with the reduction of light intensity. However, this stomatal frequency was found higher on the abaxial leaf surface from 60% shade level treatment. Moreover, only leaves from 10% shade level treatments show the formation of trichomes on both abaxial and adaxial leaf surface, and exhibited a visual symptom of severe leaf tip burn. The findings revealed that different light intensities strongly affected the morphology and growth index of C. crotalifera during ex vitro development. The usage of organic medium under 60% shade level demonstrated the most efficient method for acclimatization of micropropagated C. crotalifera plantlets

Micropropagation of an Exotic Ornamental Plant, Calathea crotalifera

A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera

Effects of different quality of soil mixture on growth development of an important medicinal plant, Boesenbergia rotunda

Growth and morphological development of Boesenbergia rotunda grown in different soil mixture were considered to determine the suitable growing media for the species. B. rotunda or fingerroot ginger is a highly important medicinal plant belonging to Zingiberaceae family. The rhizome and fingerroot structure of this species contains several bioactive compounds with various functional pharmaceutical activities. It can be vegetatively propagated through cutting rhizome and shows slow growth rate. This study provided some analysis and informative data on how the three types of typical soil (red soil (RS), black soil (BS) and sand (SS) can give important influences on the morphological and physiological development of the species. Fourteen different types of soil mixture with different mix ratio and quality were used as a growth medium. The effects of these treatments were implied based on the growth rate, evaluation in biomass quality of shoots, rhizomes and fingerroots, and photosynthetic pigment analysis. The highest quality growth of B. rotunda was established in the medium containing high percentage of RS and BS with low of SS. The growth rate of plant and photosynthetic pigment concentration were increased in the medium containing a high percentage of RS (50-100%) and BS (50-100%). The presence of a high percentage of BS in the medium was also significantly increased the biomass production of rhizome and fingerroots. The soil mixture might not make adverse effects on the shoots biomass except in medium containing more than 50% of SS. The physical characteristics (bulk density, porosity, water holding capacity and electrical conductivity) of the soil mixture were studied to determine the quality of an optimum combination of the growing medium. The synthetically evaluation index of the plant showed that the different type of soil mixture has a significant effect on growth development of B. rotunda that necessary in industrialization cultivation study

Sensory Attributes and Phytochemical Composition in Microgreens: Implications for Acceptability Among School Students in Selangor, Malaysia

Abstract. Microgreens, the young seedlings of edible plants, have gained attention for their potential health benefits and culinary applications. This newly emerging functional food crop has the potential to sustainably diversify global food systems, facilitate urbanization and climate change responses, and advance human health. However, their reception among school students remains an understudied area, particularly in the Malaysian context. In this study, five microgreens species including ulam raja, red amaranth, red radish, daikon, and corn were evaluated for their sensory perception and acceptability among secondary school students in Selangor, and their phytochemical compositions. Among the evaluated species, corn exhibited the highest intensity of sweetness, ulam raja demonstrated a strong aroma and bitterness, while red radish was notable for its astringency and heat. Red amaranth had the highest intensity of grassy taste, and all five microgreens had a low score for sourness. Corn had the highest rating on acceptability of flavor and overall eating quality, signifying its favorable impression among students. Conversely, red radish and ulam raja received the lowest acceptability scores in both categories. The highest total phenolic and flavonoid content and antioxidant activity was exhibited in ulam raja. These findings suggest the potential to leverage corn’s favorable acceptability to introduce microgreens into student diets. In general, this study highlights the correlation between sensory attributes and phytochemical composition, both influencing the Malaysian students’ perceptions of microgreens. By understanding their preferences, educators, policymakers, and entrepreneurs can formulate effective strategies for healthier eating habits among the youth and develop successful commercialization strategies. Keywords: flavor, food systems, healthy diet, microgreens, student acceptanc

Enhanced Production of Active Photosynthetic and Biochemical Molecules in L. Using Biotic and Abiotic Elicitors in Hydroponic Culture.

Elicitors are stressors that activate secondary pathways that lead to the increased production of bioactive molecules in plants. Different elicitors including the fungus Aspergillus niger (0.2 g/L), methyl jasmonate (MeJA, 100 µM/L), and silver nanoparticles (1 µg/L) were added, individually and in combination, in a hydroponic medium. The application of these elicitors in hydroponic culture significantly increased the concentration of photosynthetic pigments and total phenolic contents. The treatment with MeJA (methyl jasmonate) (100 µM/L) and the co-treatment of MeJA and AgNPs (silver nanoparticles) (100 µM/L + 1 µg/L) exhibited the highest chlorophyll a (29 µg g-1 FW) and chlorophyll b (33.6 µg g-1 FW) contents, respectively. The elicitor MeJA (100 µM/L) gave a substantial rise in chlorophyll a and b and total chlorophyll contents. Likewise, a significant rise in carotenoid contents (9 µg/g FW) was also observed when subjected to meJA (100 µM/L). For the phenolic content, the treatment with meJA (100 µM/L) proved to be very effective. Nevertheless, the highest production (431 µg/g FW) was observed when treated with AgNPs (1 µg/L). The treatments with various elicitors in this study had a significant effect on flavonoid and lignin content. The highest concentration of flavonoids and lignin was observed when MeJA (100 mM) was used as an elicitor, following a 72-h treatment period. Hence, for different plant metabolites, the treatment with meJA (100 µM/L) and a co-treatment of MeJA and AgNPs (100 µM/L + 1 µg/L) under prolonged exposure times of 120-144 h proved to be the most promising in the accretion of valuable bioactive molecules. The study opens new insights into the use of these elicitors, individually or in combination, by using different concentrations and compositions

Enhanced Production of Active Photosynthetic and Biochemical Molecules in <i>Silybum marianum</i> L. Using Biotic and Abiotic Elicitors in Hydroponic Culture

Elicitors are stressors that activate secondary pathways that lead to the increased production of bioactive molecules in plants. Different elicitors including the fungus Aspergillus niger (0.2 g/L), methyl jasmonate (MeJA, 100 µM/L), and silver nanoparticles (1 µg/L) were added, individually and in combination, in a hydroponic medium. The application of these elicitors in hydroponic culture significantly increased the concentration of photosynthetic pigments and total phenolic contents. The treatment with MeJA (methyl jasmonate) (100 µM/L) and the co-treatment of MeJA and AgNPs (silver nanoparticles) (100 µM/L + 1 µg/L) exhibited the highest chlorophyll a (29 µg g−1 FW) and chlorophyll b (33.6 µg g−1 FW) contents, respectively. The elicitor MeJA (100 µM/L) gave a substantial rise in chlorophyll a and b and total chlorophyll contents. Likewise, a significant rise in carotenoid contents (9 µg/g FW) was also observed when subjected to meJA (100 µM/L). For the phenolic content, the treatment with meJA (100 µM/L) proved to be very effective. Nevertheless, the highest production (431 µg/g FW) was observed when treated with AgNPs (1 µg/L). The treatments with various elicitors in this study had a significant effect on flavonoid and lignin content. The highest concentration of flavonoids and lignin was observed when MeJA (100 mM) was used as an elicitor, following a 72-h treatment period. Hence, for different plant metabolites, the treatment with meJA (100 µM/L) and a co-treatment of MeJA and AgNPs (100 µM/L + 1 µg/L) under prolonged exposure times of 120–144 h proved to be the most promising in the accretion of valuable bioactive molecules. The study opens new insights into the use of these elicitors, individually or in combination, by using different concentrations and compositions

Enhanced Production of Active Photosynthetic and Biochemical Molecules in L. Using Biotic and Abiotic Elicitors in Hydroponic Culture.

Elicitors are stressors that activate secondary pathways that lead to the increased production of bioactive molecules in plants. Different elicitors including the fungus Aspergillus niger (0.2 g/L), methyl jasmonate (MeJA, 100 µM/L), and silver nanoparticles (1 µg/L) were added, individually and in combination, in a hydroponic medium. The application of these elicitors in hydroponic culture significantly increased the concentration of photosynthetic pigments and total phenolic contents. The treatment with MeJA (methyl jasmonate) (100 µM/L) and the co-treatment of MeJA and AgNPs (silver nanoparticles) (100 µM/L + 1 µg/L) exhibited the highest chlorophyll a (29 µg g-1 FW) and chlorophyll b (33.6 µg g-1 FW) contents, respectively. The elicitor MeJA (100 µM/L) gave a substantial rise in chlorophyll a and b and total chlorophyll contents. Likewise, a significant rise in carotenoid contents (9 µg/g FW) was also observed when subjected to meJA (100 µM/L). For the phenolic content, the treatment with meJA (100 µM/L) proved to be very effective. Nevertheless, the highest production (431 µg/g FW) was observed when treated with AgNPs (1 µg/L). The treatments with various elicitors in this study had a significant effect on flavonoid and lignin content. The highest concentration of flavonoids and lignin was observed when MeJA (100 mM) was used as an elicitor, following a 72-h treatment period. Hence, for different plant metabolites, the treatment with meJA (100 µM/L) and a co-treatment of MeJA and AgNPs (100 µM/L + 1 µg/L) under prolonged exposure times of 120-144 h proved to be the most promising in the accretion of valuable bioactive molecules. The study opens new insights into the use of these elicitors, individually or in combination, by using different concentrations and compositions