Married Men's Experiences of Domestic Violence on their Wives: A Qualitative Study

Introduction: Violence against women is a universal phenomenon experienced in public and private areas. Assessing the experiences of domestic violence and determining its causes is essential to identify, prevent, and control it. This study was performed to designate married men's experiences of domestic violence on their wives in Sabzevar City, Iran. Method: In this descriptive qualitative study, participants were selected using purposive sampling, and it continued based on snowball and theoretical sampling. A face-to-face semi-structured interview was used to collect the data. A total of 20 participants (15 men, 2 spouses, 2 consultants, and 1 mother-in-law) were interviewed in 8 months. Interviews were analyzed using Graham and Landman’s content analysis method. To ensure consistency and accuracy of data, credibility, transferability, dependability, and conformability criteria were assessed. Results: Three main categories were conceptualized including “unfriendly interaction”, “lack of complying couple’s emotional needs”, and “lack of complying husband’s sexual needs”. Conclusion: Based on results, designing interventions to establish friendly interaction between couples, helping couples to identify each other's emotional and sexual needs, and couples’ effort to meet each other's emotional and sexual needs can be effective in prevention of violence on women. Keywords: Qualitative research, Domestic violence, Married persons, Men, Life experiences, Ira

RNAi and miRNA in Viral Infections and Cancers

Since the first report of RNA interference (RNAi) less than a decade ago, this type of molecular intervention has been introduced to repress gene expression in vitro and also for in vivo studies in mammals. Understanding the mechanisms of action of synthetic small interfering RNAs (siRNAs) underlies use as therapeutic agents in the areas of cancer and viral infection. Recent studies have also promoted different theories about cell-specific targeting of siRNAs. Design and delivery strategies for successful treatment of human diseases are becomingmore established and relationships between miRNA and RNAi pathways have been revealed as virus-host cell interactions. Although both are well conserved in plants, invertebrates and mammals, there is also variabilityand a more complete understanding of differences will be needed for optimal application. RNA interference (RNAi) is rapid, cheap and selective in complex biological systems and has created new insight sin fields of cancer research, genetic disorders, virology and drug design. Our knowledge about the role of miRNAs and siRNAs pathways in virus-host cell interactions in virus infected cells is incomplete. There are different viral diseases but few antiviral drugs are available. For example, acyclovir for herpes viruses, alpha-interferon for hepatitis C and B viruses and anti-retroviral for HIV are accessible. Also cancer is obviously an important target for siRNA-based therapies, but the main problem in cancer therapy is targeting metastatic cells which spread from the original tumor. There are also other possible reservations and problems that might delay or even hinder siRNA-based therapies for the treatment of certain conditions; however, this remains the most promising approach for a wide range of diseases. Clearly, more studies must be done to allow efficient delivery and better understanding of unwanted side effects of siRNA-based therapies. In this review miRNA and RNAi biology, experimental design, anti-viral and anti-cancer effects are discussed

RNAi and miRNA in viral infections and cancers

Since the first report of RNA interference (RNAi) less than a decade ago, this type of molecular intervention has been introduced to repress gene expression in vitro and also for in vivo studies in mammals. Understanding the mechanisms of action of synthetic small interfering RNAs (siRNAs) underlies use as therapeutic agents in the areas of cancer and viral infection. Recent studies have also promoted different theories about cell-specific targeting of siRNAs. Design and delivery strategies for successful treatment of human diseases are becomingmore established and relationships between miRNA and RNAi pathways have been revealed as virus-host cell interactions. Although both are well conserved in plants, invertebrates and mammals, there is also variabilityand a more complete understanding of differences will be needed for optimal application. RNA interference (RNAi) is rapid, cheap and selective in complex biological systems and has created new insight sin fields of cancer research, genetic disorders, virology and drug design. Our knowledge about the role of miRNAs and siRNAs pathways in virus-host cell interactions in virus infected cells is incomplete. There are different viral diseases but few antiviral drugs are available. For example, acyclovir for herpes viruses, alpha-interferon for hepatitis C and B viruses and anti-retroviral for HIV are accessible. Also cancer is obviously an important target for siRNA-based therapies, but the main problem in cancer therapy is targeting metastatic cells which spread from the original tumor. There are also other possible reservations and problems that might delay or even hinder siRNA-based therapies for the treatment of certain conditions; however, this remains the most promising approach for a wide range of diseases. Clearly, more studies must be done to allow efficient delivery and better understanding of unwanted side effects of siRNA-based therapies. In this review miRNA and RNAi biology, experimental design, anti-viral and anti-cancer effects are discussed

Antiviral Activity of Sirna UL42 against Herpes Simplex Virus Type 1 in HeLa Cell Culture

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. In this study, we applied siRNAs targeting the UL42 gene of human herpes simplex virus type 1 (HSV-1), which encodes a multifunctional polypeptide that is vital for virus DNA replication, binding to DNA, stably associating with the virus DNA polymerase (Pol), and acting to increase the length of DNA chains synthesized by Pol. HeLa cell line was used for HSV 1 infection and SiRNA transfection was done to suppress UL-42 gene in cell culture. The decrease in titer of HSV 1 was observed by rReal Time PCR to detect the drop in HSV 1 DNA synthesis and translation. The inhibition rates of siRNA1 and siRNA2 on HSV-1 plaque formation were reported and Comparing with virus control, siRNA1 and siRNA2 reduced DNA replication HSV-1. The decision whether the decrease in the number of HSV-1 plaques was due to siRNA silencing expression of the UL42 gene, a real-time PCR indicating that UL42-specific siRNAs blocked the expression of the UL42 gene significantly. Comparing with virus control, siRNA1 and siRNA2 reduced the expression of UL42 gene. In this study the synthetic siRNA silenced UL42 mRNA expression effectively and specifically and inhibited HSV-1 replication and also our data offer new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication

Evaluation of Human Papilloma Virus Infection in Patients with Esophageal Squamous Cell Carcinoma from the Caspian Sea Area, North of Iran

Introduction: HPV has been found repeatedly in esophageal squamous cell carcinoma (ESCC) tissues. However, reported detection rates of HPV DNA in these tumors have varied markedly. Differences in detection methods, sample types, and geographic regions of sample origin have been suggested as potential causes of variation. We have reported that infection of HPV DNA in ESCC tumors depends on anatomical sites of esophagus of the patients from Mazandaran, north of Iran. Materials and Methods: HPV DNA was examined in 46 upper, 69 middle and 62 lower third anatomical sites of esophageal squamous cell carcinoma specimens collected from Mazandaran province in north Iran, near the Caspian Littoral as a region with high incidence of ESCC. HPV L1 DNA was detected using Qualitative Real time PCR and MY09/MY11 primers. Results: 28.3% of upper, 29% of middle and 25.8% of lower third of ESCC samples were positive for HPV DNA. 13.6% for males and 14.1% for females were HPV positive in all samples. Conclusions: HPV infection is about one third of ESCC in this area. Findings in this study increase the possibility that HPV is involved in esophageal carcinogenesis. Further investigation with a larger sample size is necessary

High-Risk and Low-Risk Human Papillomavirus in Esophageal Squamous Cell Carcinoma at Mazandaran, Northern Iran

Cancers are the second most common cause of nonaccidental deaths in Iran, following cardiovascular deaths. Mazandaran, near the Caspian Littoral at north of Iran have identified as a several-high incidence area for Esophageal Squamous Cell Carcinoma (ESCC) in the world. Several associated risk factors, such as dietary and cultural habits, infectious agents, nutritional deficiencies, too much use of tobacco and alcohol and infection to certain DNA tumor viruses (HPVs), including environmental and genetic factors are attributed to this disease. To explore this issue, we analyzed HPV DNA prevalence and HPV types together in relation to tumor sites a high-incidence population. Archived tissue blocks from 46, 69 and 62 upper, middle and lower third of esophagus, respectively from ESCC patients were evaluated for the presence of HPV DNA by PCR using the degenerate HPV L1 consensus primer pairs MY09/MY11. The positive specimens were evaluated by Real-time PCR to determine HPV genotypes. From the 49 HPV positive cases, of ESCC patients, 5 (23.1%), 11 (55 %) and 9 (56.3 %) of upper, middle and lower third of ESCC specimens, respectively were positive by at least one high and one low-risk HPV genotypes. In general, HPV45 and HPV11 were the most common high- risk and low-risk HPV genotypes in HPV L1 positive cases, respectively, followed by HPV6, HPV52 and HPV39. Therefore, the high prevalence of HPV DNA in different anatomical sites of ESCC patients from the Mazandaran region in North of Iran provides more evidence for a role of HPV in this cancer

Change processes questionnaire for treatment of drug dependents based on transtheoretical model: Psychometric properties in Iran 2016

Background: Drug abuse is a serious subject that can lead to social, economic, cultural and health problems. The routine approaches in treatment of drug abuse are either medication or non-medication methods. Non-medication approach is focused on mental and social health. Non-drug treatment requires appropriate tools. Objective: To determine the validity, reliability, and structure of the Processes of Change Questionnaire. Methods: In this cross-sectional study, which was conducted in 2016, three hundred sixty-two drug dependents in Sabzevar Substance Abuse Treatment Centers (Iran) were enrolled in the study. Samples were selected through the multi-stage sampling method. They completed the 40-item Processes of Change Questionnaire (drug version). In this study, Backward-Forward method, confirmatory factor analysis (CFA), test-retest, Cronbach's alpha, content validity ratio (CVR) and content validity index (CVI) were used, using AMOS 5.0 and SPSS version 11.5. Results: Findings of the CFA showed the questionnaire has a good fit. The fit index of the CFA was calculated as 0.75. Cronbach’s alpha was 0.93 for the whole questionnaire, and ranged from 0.51 to 0.88 for its different domains. Also, intraclass correlation coefficient (ICC) and content validity were satisfactory. Conclusion: Based on the findings of this study, the processes of change 40-item questionnaire: drug version was a credible, reliable and valid tool to identify effective factors in the treatment drug abuse among the population of the Sabzevar, Iran

Review of the Leak-off Tests with a Focus on Automation and Digitalization

The drilling and research communities are leading the way toward more digitally-controlled operations to ensure that the drilling process takes place as safely and gently as possible with the lowest possible carbon footprint. Today’s cutting-edge operations are run on large high-performance drilling installations where operations are largely run remotely from the driller’s operating station. Digitalization of the drilling process is the goal for performing drilling operations remotely from onshore. Leak-off test (LOT) or extended leak-off test (XLOT) plays a critical role in the petroleum industry. Therefore, recognizing all affecting parameters on LOT/XLOT and Formation integrity test (FIT) performance is vital. Because, in some cases, it is not possible to fully understand what happened during the test, having a deep insight into the LOT procedure is very important. One of the current study's main objectives is to thoroughly explain all stages of these tests and assemble all the significant parameters. Thus, many scientific papers on these tests were deeply reviewed and were classified into four main groups focusing on the application of LOT/XLOT (i) in stress estimation and geomechanical studies, (ii) concerning hydraulic fracturing, (iii) concerning wellbore stability, and (iv) numerical modeling, and then, the corresponding discussions were conducted. It was found that in-situ stress estimation is the most common application of the leak-off test. Moreover, considering the importance of LOT and the desire to digitize operations in the oil and gas industry, it was found that the automatic LOT/XLOT is a fully required approach. The primary purpose of this study, which is hence considered its main contribution, is to prepare a LOT flowchart that would set off the further code development tasks of the field. The fundamental code of the present study was written and checked using a real dataset in a Python environment. The results were satisfying and indicated a successful start, which lays a foundation for future automated LOT/XLOT tests