Non-lethal PCR genotyping of single Drosophila

In Drosophila, genetic techniques relying on stochastic chromosomal rearrangements involve the generation and screening of a large number of fly stocks to isolate a few lines of interest. Here, we describe a PCR-based method allowing non-lethal molecular characterization of single flies. Using this procedure, individual candidate recombinant animals can be genotyped and selected one generation earlier than with extant methodology and, importantly, before stocks are established. This advance should significantly facilitate several of the most fundamental and routine techniques in Drosophila genetics

An extreme rotation measure in the high-redshift radio galaxy PKS B0529-549

We present the results of a radio polarimetric study of the high-redshift radio galaxy PKS B0529-549 (z=2.575), based on high-resolution 12 mm and 3 cm images obtained with the Australia Telescope Compact Array (ATCA). The source is found to have a rest-frame Faraday rotation measure of -9600 rad m^{-2}, the largest seen thus far in the environment of a z > 2 radio galaxy. In addition, the rest-frame Faraday dispersion in the screen responsible for the rotation is calculated to be 5800 rad m^{-2}, implying rotation measures as large as -15400 rad m^{-2}. Using supporting near-IR imaging from the Very Large Telescope (VLT), we suggest that the rotation measure originates in the Ly-alpha halo surrounding the host galaxy, and estimate the magnetic field strength to be ~10 microGauss. We also present a new optical spectrum of PKS B0529-549 obtained with the New Technology Telescope (NTT), and propose that the emission-line ratios are best described by a photoionization model. Furthermore, the host galaxy is found to exhibit both hot dust emission at 8.0 microns and significant internal visual extinction (~1.6 mag), as inferred from Spitzer Space Telescope near/mid-IR imaging.Comment: 12 pages, 6 figures, accepted for publication in MNRA

MILITARY HABEAS CORPUS: I

The mobilization of over twelve million persons into the armed forces in World War II made necessary a vastly expanded resort to court martial proceedings to enforce the criminal law. The trial by military tribunals of civilian employees of the military establishment in overseas areas and of prisoners of war and war crimes defendants added substantially to the number confined by military authority. On January 31, 1950, there remained in federal penal institutions 2508 prisoners serving civilian type felony sentences imposed by military tribunals. Before World War II, legal problems arising from attempts to invoke the remedy of habeas corpus by military prisoners were rare and were primarily of historical and academic interest. In the past five years the quantitative pressure of this military prisoner population has produced a substantial volume of case law in the field of military habeas corpus, has caused the United States Supreme Court to review the subject, and has made it one of practical interest to the private practitioner as well as the military lawyer

MILITARY HABEAS CORPUS: II

The doctrine is well established that habeas corpus is an extraordinary remedy which will not ordinarily lie where the law has provided another remedy. The numerical pressure of habeas corpus petitions by all types of prisoners in recent years has reached such proportions as to constitute a major problem in the administration of justice. It has engaged the administrative consideration of judicial officers and been the subject of legislation both federal and state. The Chief Justice of the United States in an address before the American Bar Association on September 7, 1949 strikingly stated the problem and urged that something should be done to stem the flow

The Predicted Binding Site and Dynamics of Peptide Inhibitors to the Methuselah GPCR from Drosophila melanogaster

Peptide inhibitors of Methuselah (Mth), a G protein-coupled receptor (GPCR), were reported that can extend the life span of Drosophila melanogaster. Mth is a class B GPCR, which is characterized by a large, N-terminal ectodomain that is often involved with ligand recognition. The crystal structure of the Mth ectodomain, which binds to the peptide inhibitors with high affinity, was previously determined. Here we report the predicted structures for RWR motif peptides in complex with the Mth ectodomain. We studied representatives of both Pro-class and Arg-class RWR motif peptides and identified ectodomain residues Asp139, Phe130, Asp127, and Asp78 as critical in ligand binding. To validate these structures, we predicted the effects of various ligand mutations on the structure and binding to Mth. The binding of five mutant peptides to Mth was characterized experimentally by surface plasmon resonance, revealing measured affinities that are consistent with predictions. The electron density map calculated from our MD structure compares well with the experimental map of a previously determined peptide/Mth crystal structure and could be useful in refining the current low-resolution data. The elucidation of the ligand binding site may be useful in analyzing likely binding sites in other class B GPCRs