165 research outputs found
Activity and Process Stability of Purified Green Pepper (Capsicum annuum) Pectin Methylesterase
Pectin methylesterase (PME) from green bell peppers (Capsicum annuum) was extracted and purified by affinity chromatography on a CNBr-Sepharose-PMEI column. A single protein peak with pectin methylesterase activity was observed. For the pepper PME, a biochemical characterization in terms of molar mass (MM), isoelectric points (pI), and kinetic parameters for activity and thermostability was performed. The optimum pH for PME activity at 22 °C was 7.5, and its optimum temperature at neutral pH was between 52.5 and 55.0 °C. The purified pepper PME required the presence of 0.13 M NaCl for optimum activity. Isothermal inactivation of purified pepper PME in 20 mM Tris buffer (pH 7.5) could be described by a fractional conversion model for lower temperatures (55?57 °C) and a biphasic model for higher temperatures (58?70 °C). The enzyme showed a stable behavior toward high-pressure/temperature treatments. Keywords: Capsicum annuum; pepper; pectin methylesterase; purification; characterization; thermal and high-pressure stabilit
Cognitive Effect Driving Game - Experiment 1 - virDRT data
The data contained herein are the results of the virtual detection response task (virDRT) developed during the Cognitive-effect driving game (CEDG
Cognitive Effect Driving Game - Experiment 1 - EEG Data
This Collection is the EEG Performance Measure Data from the Emotiv Epoc X headset and software
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