Effect Of Fibroblast Growth Factor On Oxidative Events In Wound Tıssue

Yara iyileşmesi kompleks bir süreçtir ve oksidatif olaylar yoğun olarak kendisini gösterir. Büyüme faktörlerinden biri olan bazik fibroblast büyüme faktörü (bFGF)'nin yara iyileşmesindeki oksidatif olaylarla olan ilişkisi açık değildir. İyileşmenin özellikle proliferatif fazında etkili olan bFGF'nin 3. ve 7. gününde 10 ng/ml uygulanmasıyla diyabetik (hiperglisemik) deney hayvanlarında eksizyonal olarak oluşturulan yaralarda kontrol gruplarına oranla iyileşmede gelişme göstermesi temel araştırma odağımızdır.Bu amaçla, 30 adet Wistar albino erkek rat streptozosin (STZ) (ip, 60 mg/kg) ile hiperglisemik yapılmış ve deney grupları tedavi edilmeyen ve bFGF ile tedavi edilen alt gruplarına ayrılmıştır. bFGF ile tedavi edilen gruplardaki ratların yaralarına topikal yolla bFGF (10 ng/ml) uygulanmıştır. Uygulamalardan sonra iyileşmenin 3. ve 7. günlerinde ratlar feda edilmiş ve çıkarılan yara dokularında bFGF' nin normal ve geciken yara iyileşmesi üzerine olan oksidatif etkilerini araştırmak amacıyla malondialdehit (MDA), nitrik oksit (NO), askorbik asit(AA), süperoksit dismutaz (SOD) ve glutatyon (GSH) seviyeleri ölçülmüştür. Sonuçlar aritmetik ortalama ±standart hata olarak ifade edilmiş ve Anova Varyans Analizi ile değerlendirilmiştir(p<0,05).Sonuç olarak, bFGF uygulanan yaraların yara kapanma oranları karşılaştırıldığında; bFGF uygulanan grupların yaralarındaki iyileşme oranı tedavisiz gruplara göre artış göstermiştir. Tedavili ve tedavisiz gruplar karşılaştırıldığında (7. günde) ratlarda bFGF uygulamasının yara dokusu MDA düzeylerini tedavisiz gruba kıyasla istatistiksel olarak azalttığı tespit edilmiştir (p<0,05). Tedavili ve tedavisiz grupların 3. günleri karşılaştırıldığında, bFGF uygulamasının yara dokusu NOx düzeylerini tedavisiz gruplara kıyasla istatistiksel olarak arttırdığı (p<0,05), AA düzeylerini ise azalttığı tespit edilmiştir (p<0,05). bFGF'in yara iyileşmesi esnasında meydan gelen oksidatif hasarın ortadan kaldırılmasında ve yaranın inflamasyon ve proliferasyon fazında iyileşmeye katkı sağladığı söylenebilir.Wound healing is a complex process in which oxidative events are intensively manifested. Although it is known that one of the growth factors, basic fibroblast growth factor (bFGF), has positive effects on wound healing, the relationship with oxidative events in wound healing is not clear. Our main goal is that bFGF, which is effective in the proliferative phase of healing, improves excisional wound healing in the experimental animals when compared to the control group, with application of 10 ng / ml on days 3 and 7. It is also our goal to demonstrate the healing effect of bFGF in diabetic wounds.For this purpose, 30 Wistar albino male rats were hyperglycemic with STZ (ip, 60 mg / kg) and the experimental groups were divided into untreated and bFGF-treated subgroups. Healthy and diabetic rat groups are subdivided into untreated and bFGF-treated subgroups. bFGF (10 ng / ml) was administered topically to the injuries of rats in groups treated with bFGF. After the treatments, rats were sacrificed on days 3 and 7 of recovery.Malondialdehyde (MDA), nitric oxide (NO), ascorbic acid (AA), glutathione (GSH) levels and superoxide dismutase (SOD) activity were measured in order to investigate the oxidative effects of bFGF on normal and delayed wound healing in the wound tissues. The results were expressed as mean ± standard error and evaluated by Anova Variance Analysis (p<0,05).As a result, the rate of wound closure in bFGF-treated groups increased compared to the untreated groups. It was determined that bFGF administration was decreased the MDA levels of the wound tissue (p<0,05) compared to untreated groups on day 7 rats. It was determined that bFGF administration was increased statistically the wound tissue NOx levels, while was decreased AA levels on day 3 compared to the untreated group of rats (p<0,05). bFGF may contribute to the elimination of oxidative damage during wound healing and to healing both the inflammation and the proliferation phase of the woun

Conservative management of post-partum hemorrhage secondary to placenta previa-accreta with hypogastric artery ligation and endo-uterine hemostatic suture

WOS: 000394675900003PubMed ID: 27987348AimThe aim of this study was to investigate maternal and neonatal outcomes of conservative management of post-partum hemorrhage due to placenta previa-accreta using hypogastric artery ligation and endo-uterine hemostatic suture to lower uterine segment. MethodsThe records of 38 patients who were managed conservatively with hypogastric artery ligation and endo-uterine hemostatic suture to control post-partum hemorrhage secondary to placenta previa-accreta between April 2014 and January 2016, were reviewed retrospectively. Placenta previa-accreta was diagnosed according to gray-scale, color and 3-D power Doppler ultrasonography in addition to the intraoperative findings based on fragmentary or difficult separation of the placenta. In the case of conservative treatment protocol failure, cesarean hysterectomy was performed. ResultsOf these patients, 55.2% were between 25 and 35 years old; 97.5% were multiparous; 71.2% had two or more previous cesarean section and 68.5% had preterm delivery. Women with placenta accreta had a median estimated blood loss of 450 mL; 57.8% of patients had blood transfusion (mean intraoperative transfusion, 2 units packed red blood cells; range, 0-9 units). Median duration of operation was 112.5 min (range, 45-305 min) and 32 patients (84.3%) with placenta accreta did not undergo cesarean hysterectomy. ConclusionConservative treatment of post-partum hemorrhage secondary to placenta previa-accreta with hypogastric artery ligation and endo-uterine hemostatic sutures to the lower segment of the uterus is associated with lower hysterectomy rate compared with the other conservative methods reported in the literature

The Protective Effects of Glycyrrhetinic Acid and Chrysin against Ischemia-Reperfusion Injury in Rat Ovaries

Objective. To evaluate the protective effects of glycyrrhetinic acid (GA) and chrysin (CH) on experimental ischemia-reperfusion (I/R) injury in rat ovaries using tissue oxidative stress marker levels, hormone levels, and histopathological scores. Methods. Sixty healthy rats were randomly divided into six equal groups: control, I/R, I/R + CH (50 mg/kg/day), I/R + GA (100 mg/kg/day), CH (50 mg/kg/day), and GA (100 mg/kg/day). Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after CH and GA treatment. Results. The antioxidant defense system parameters were significantly higher in the ovarian tissues of the I/R + CH and I/R + GA groups than in those of the I/R group. Serum follicle-stimulating hormone levels were significantly reduced, and serum anti-Müllerian hormone levels were significantly increased in rats treated with CH and GA compared with those in the I/R group. Additionally, the histopathological scores of the I/R + CH and I/R + GA groups were significantly improved compared with those of the I/R group. Conclusions. The significant improvements in tissue oxidative stress parameters, serum hormone levels, and histological scores observed in this study indicate that treatment with CH or GA may be a conservative approach to prevent I/R injury in adnexal torsion cases after the ovarian detorsion procedure

Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model

Abstract Background In recent years, cancer rates have been rising among reproductive-age women. Thus, chemotherapy exposure has become an important cause of premature ovarian failure (POF). There has been growing interest regarding the preservation and restoration of ovarian function before and after oncological treatment because of the reproductive risk of chemotherapeutics and improved long-term survival of cancer patients. In this study, we sought to analyze the effects of curcumin (CRC) and capsaicin (CPS) on cyclophosphamide-induced POF in a rat model. Methods POF in rats was induced by intraperitoneal injection of 200 mg/kg cyclophosphamide on day 1 and then 8 mg/kg/day for the following 14 days. After 14 days of cyclophosphamide administration, rats were randomly divided into three groups as follows (n = 10/group): POF, POF + CRC (100 mg/kg/day), and POF + CPS (0.5 mg/kg/day) to determine the effects of CRC and CPS on the cyclophosphamide-induced POF rat model. Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after the CRC and CPS treatments. Results Malonaldehyde levels were significantly reduced, and glutathione levels and superoxide dismutase activity were significantly increased, in ovarian tissues in the POF + CRC and POF + CPS groups compared with the POF group. In the POF group, we observed hemorrhage and prominent mononuclear cell infiltration beneath the germinative epithelium, vascular congestion in ovarian stroma, hemorrhage around the corpus luteum, and atresia in ovarian follicles. This histopathological damage was significantly improved by treatment with CRC and CPS. There was a significant reduction in serum follicle-stimulating hormone and luteinizing hormone levels in rats treated with CRC and CPS compared with the POF group. Moreover, the levels of estradiol and anti-mullerian hormone in rats treated with CRC and CPS were significantly increased compared with the control group. Conclusions In conclusion, CRC and CPS treatment of rats with cyclophosphamide-induced POF had a beneficial effect on reducing ovarian damage by improving tissue oxidative stress marker levels, ovarian reserve marker levels, and histopathological parameters. The significant improvements in ovarian tissue histopathological damage and hormonal levels detected in this study indicate that treatment with CRC or CPS might be a conservative treatment approach for cyclophosphamide-induced POF