The importance of p16 and CD117 expression in melanocytic lesions

Aim: The present study aims to determine the p16 and CD117 expression profiles of melanocytic lesions to investigate immune profiles that may facilitate differential diagnosis of melanoma from benign or potential precursor melanocytic lesions.Materials and Methods: Immunohistochemistry for p16 and CD117 was applied in a total of 81 cases with melanocytic lesions.Results: A significant loss of p16 expression was found in melanoma cases compared to benign and precursor melanocytic lesions (p<0.05). Moreover, a significant loss of p16 expression was also noted in cases of dysplastic nevus compared to those with intradermal nevus (p<0.01). While no CD117 expression was observed in intradermal nevi, high-level expression was seen in cases with Spitz nevus, blue nevus, invasive melanoma and dysplastic nevus (p<0.01).Conclusion: We believe using p16 and CD117 together may provide an important marker combination to aid in distinguishing melanoma from benign lesions and benign lesions from potential precursor melanocytic lesions

Are fungi and EBV effective in cholesteatoma etiology?

Aim: Cholesteatoma is a commonly seen disease whose pathogenesis remains unknown. Although not a neoplastic process, it may progress to a fatal condition with local bone destruction. In this study, we aimed to present new insights concerning the etiology of cholesteatoma triggered by an inflammatory process.Materials and Methods: The study included 34 patients diagnosed with cholesteatoma upon mastoidectomy performed between 2011-2019. Due to a provisional diagnosis of cholesteatoma. The cases were investigated for the latent membrane protein (LMP-1) encoded by the Epstein-Barr Virus (EBV) using the immunohistochemical method and for the presence of fungi using Grocott’s methenamine silver (GMSII) stain.Results: No fungi was detected in any of the 34 patients by GMSII staining. Thirty-two of the 34 patients were negative with but a suspicious result was seen in 2 patients with the immunohistochemical EBV antibody. EBV-encoded RNA (EBER) analysis was applied to these 2 cases with the silver in situ hybridization method and no reaction was observed.Conclusion: In our study, we investigated the presence of fungi and EBV, which can trigger the inflammatory process. However, no EBV or fungi was detected in the tissues. Our study is the first to investigate the presence of EBV and fungi in formalin-fixed tissue in cases of aggressive cholesteatoma

The Effect of EGFR, P16 and Ki67 Expression on Prognosis in Head and Neck Squamous Cell Carcinoma

Objective: HPV and EGFR expression status may be utilized as an independent prognostic factor owing to the different clinical and demographic characteristics head and neck cancers. In the study, it was aimed to investigate the association between EGFR, p16 and ki67 expression and survival in patients with head and neck squamous cell carcinoma (SCC).Materials and Methods: A total of 43 patients with SCC of the head and neck region were included in the study. EGFR, p16 and Ki67 were examined by means of immunohistochemistry. The association between these markers and survival was investigated.Results: EGFR expression was detected in 14 cases (32.5%), Staining with p16 was positive in 20 cases (46.5%). Mean duration of follow up was 32 months. There was a statistically significant difference between ki67 proliferation indices of patients who survived and those who died (p=0.037). Survival was significantly shorter in EGFR positive patients compared to those negative for EGFR expressions (p=0.037). Mean survival was 30 months in the 20 p16 positive patients and 33.5 months in p16 negative patients (p=0.847).Conclusion: This study supports that EGFR and Ki67 may be important markers to predict prognosis and survival in patients with head and neck SCC.

Renal Cell Carcinoma Metastasis with Nasal Cavity

Renal cell carcinoma is an aggressive tumor that accounts for 3% of adult neoplasia. The most common sites of metastasis are the lung, adrenal, large intestine, brain and intraabdominal organs. In addition, cases of metastasis in the heart, bladder, breast, tonsil and thyroid have been reported in the literature. We present a case of renal cell carcinoma metastasis located in a nasal cavity in order to emphasize the possibility of metastatic lesions being responsible for nasal obstruction and epistaxis

Renal Cell Carcinoma Metastasis with Nasal Cavity

Renal hücreli karsinom, erişkin neoplazilerinin %3’ünü oluşturan agresif bir tümördür. En sık metastaz yaptığı yerler akciğer, adrenal, barsak, beyin ve intraabdominal organlardır. Bunun yanı sıra literatürde kalp, mesane, meme, tonsil ve tiroide yerleşen metastaz olguları bildirilmiştir. Bu yazıda bir nazal kavite yerleşimli renal hücreli karsinom metastazı olgusunu, nazal obstrüksiyon ve epistaksis görülen ve renal hücreli karsinomu olan hastalarda metastaz olasılığının akılda tutulmasını vurgulamak amacıylasunduk.Renal cell carcinoma is an aggressive tumor that accounts for 3% of adult neoplasia. The most common sites of metastasis are the lung, adrenal, large intestine, brain and intraabdominal organs. In addition, cases of metastasis in the heart, bladder, breast, tonsil and thyroid have been reported in the literature. We present a case of renal cell carcinoma metastasis located in a nasal cavity in order to emphasize the possibility of metastatic lesions being responsible for nasal obstruction and epistaxis

Immunohistochemical expression of E-Cadherin and ?-catenin in prostate adenocarcinoma and benign prostate hyperplasia

Disruption of the E cadherin mediated complex due to loss or depletion of E cadherin results in epithelial abnormalities and serious developmental impairment in varioustissues and organs. The present study aims to determine E cadherin and ? catenin expression in patients diagnosed with benign prostatic hyperplasia (BPH) and prostatecarcinoma (PCa) based on Gleason scores and investigate the association of these proteins with PSA levels and Gleason scoring. Immunohistochemical staining for Ecadherin and ? catenin was performed in 59 patients diagnosed with PCa and 30 patients with BPH. Mean E cadherin expression was 3.00 in patients diagnosed with BPHand 2.38±0.5 in patients with PCa, with a statistically significant difference between these values (p<0.001). Comparison of PCa cases with PSA <10 versus those withPSA ?10 revealed significantly reduced ? catenin expression in the group with PSA levels ?10 (p<0.001). Loss of E cadherin and ? catenin is known to contribute to thepathogenesis of PCa. We believe that future molecular studies on this subject may further elucidate the association between carcinoma development and the expression ofthese molecules, leading to new therapeutic targets in the treatment of PCa

Effects Of The Slab Discontinuties On Structural Systems Behavior In Reinforcement Buildings

Tez (Yüksek Lisans) -- İstanbul Teknik Üniversitesi, Fen Bilimleri Enstitüsü, 2007Thesis (M.Sc.) -- İstanbul Technical University, Institute of Science and Technology, 2007Bu çalışmada, belirli oranlarda döşeme boşluğu bulunduran betonarme yapıların deprem yükü altındaki davranışları incelenmiştir. Afet Bölgelerinde Yapılacak Yapılar Hakkında Yönetmelik’te, kat brüt alanının 1/3’ünden büyük döşeme boşluklarından kaçınılması tavsiye edilmektedir. Aksi takdirde döşeme süreksizliklerinden dolayı planda A2 türü düzensizlik oluşacağı ve döşemelerin yatay düzlemde rijit diyafram olarak çalışmadığının esas alınması ifade edilmektedir. Bu tür düzensizlik içeren binalarda birinci ve ikinci derece deprem bölgelerinde kat döşemelerinin kendi düzlemleri içinde deprem kuvvetlerini düşey taşıyıcı elemanları arasında güvenle aktarabildiğinin tahkikini istemektedir. Çalışmada; deprem ve depremin yapılar üzerindeki etkisi hakkında bilgi verilmiş, depreme dayanıklı yapı tasarımından bahsedilmiştir. ABYYHY’2007 de adı geçen döşeme düzensizliği oluşturan durumlar, bu düzensizliği içeren binalarda kullanılması gereken hesap yöntemleri ve yaptırımları anlatılmıştır. Ayrıca çeşitli ülkelerin deprem yönetmelikleri incelenmiş ve döşeme düzensizlikleri ile ilgili getirdiği şartlara değinilmiştir. Yapı düzensizlikleri ve özellikle döşeme süreksizliklerine yer verilmiştir. Oluşturulan modellerde boşlukların değişik yer ve oranları için incelemeler yapılmış, kat adedinin, kiriş sürekliliğinin, deprem bölgesinin ve zemin cinsinin değişmesi de dikkate alınmıştır. Yapıların diyafram çalışması durumuna bakılarak yönetmeliğin belirlediği sınır değerin güvenilirliği incelenmiştir. Bu incelemelere ait değerler ve grafikler sunulmuştur. Bu tez çalışmasından elde edilen sonuçlar verilmiştir.In this study, the behavior of structures with hollow concrete slabs subjected to earthquakes was investigated. In Turkish Earthquake Resistant Design Code, it is suggested to avoid hollow slabs more than 1/3 of floor gross area. Otherwise it is expressed that irregularities would occur because of the slab discontinuities and the slabs could not work as a rigid diaphragm. Moreover, the structures which contain slab discontinuities must be investigated if they transfer earthquake forces to perpendicular carriers in first and second earthquake religions safely. In this study, information about background of earthquake motion and its effects to structures were given includes irregularities in structures especially floor discontinuities. The conditions about slab discontinuities which are included in Turkish Earthquake Resistant Design Code, formulation and methodology were explained in models with hollow parts at different locations and at different hollow ratios slab are analyzed and the results are compared. The findings and conclusions are presented. Besides, the Earthquake ordinances of other countries are inspected and mentioned that obligations of them. The structure irregularity and especially slab discontinuities are placed in this study. Slab discontinuities ratios and places are inspected in generating models also beam continuous and ground variations are considered. Integrity of limit value that shown at ordinance has checked by rigid diaphragm theory. Graphs and values that obtained this study are shown. Results of this thesis have given.Yüksek LisansM.Sc

The Impact of Interventions Based on Mishel’s Uncertainty in Illness Theory on Caregivers of Children Diagnosed with Cancer: Randomized Controlled Trial

Aim: To investigate the effects of nursing interventions based on Mishel’s Uncertainty in Illness Theory (MUIT) on uncertainty, hopelessness, coping and adaptation for care givers of children diagnosed with cancer. Materials and Methods: The study was randomized controlled experimental design study, which had repeated measures (pretest-posttest-follow up) and parallel group (intervention-control), it included measures of uncertainty (PPUS), hopelessness (BHS), coping and adaptation (CAPS). The study was conducted in January-July 2019 with the caregivers of 46 children diagnosed with cancer who followed in paediatric oncology-haematology clinics. Intervention Group (IG) was given 6 modules education and consultancy according to protocol that was developed based on theory. The CONSORT guideline was performed in the study. Results: PPUS post-test mean score of IG was lower than CG (

A survey and design study of a protective cycling top wear

© 2021 Slovak University of Technology in Bratislava. All rights reserved.Cycling is a popular sport which is preferred by all age groups all around the world. As it is an active sport, it requires special types of clothing to provide comfort to the wearer. In spite being a healthy activity, cycling can result with acute or chronic injuries. In this study, an extensive survey was conducted to the cyclists in order to reveal their preferences of cycling clothes, their requirements, their accidental experiences and safety needs. By considering the survey results, optional designs of a more protective and functional cycling top wear were outlined. In order to avoid from acute injuries which were stated to be in the shoulder and arm areas the most, a para-aramid and spacer/silicon foam supported design was proposed. Also, the visibility was enhanced by the implementation of active lightening on the front and back sides of the clothing