Comparative Genomics Analysis of a New Exiguobacterium Strain from Salar de Huasco Reveals a Repertoire of Stress-Related Genes and Arsenic Resistance

Indexación: Web of Science; Scopus.The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00456/ful

In the dedicated pursuit of dedicated capital: restoring an indigenous investment ethic to British capitalism

Tony Blair’s landslide electoral victory on May 1 (New Labour Day?) presents the party in power with a rare, perhaps even unprecedented, opportunity to revitalise and modernise Britain’s ailing and antiquated manufacturing economy.* If it is to do so, it must remain true to its long-standing (indeed, historic) commitment to restore an indigenous investment ethic to British capitalism. In this paper we argue that this in turn requires that the party reject the very neo-liberal orthodoxies which it offered to the electorate as evidence of its competence, moderation and ‘modernisation’, which is has internalised, and which it apparently now views as circumscribing the parameters of the politically and economically possible

Identification of the Neogenin-Binding Site on the Repulsive Guidance Molecule A

Repulsive guidance molecule (RGM) is a membrane-bound protein that was originally identified as an axon guidance molecule in the chick retinotectal system. RGMa, one of the 3 isoforms found in mammals, is involved in laminar patterning, cephalic neural tube closure, axon guidance, and inhibition of axonal regeneration. In addition to its roles in the nervous system, RGMa plays a role in enhancing helper T-cell activation. Binding of RGM to its receptor, neogenin, is considered necessary to transduce these signals; however, information on the binding of RGM to neogenin is limited. Using co-immunoprecipitation studies, we have identified that the RGMa region required for binding to neogenin contains amino acids (aa) 259–295. Synthesized peptide consisting of aa 284–293 directly binds to the extracellular domain (ECD) of recombinant neogenin, and addition of this peptide inhibits RGMa-induced growth cone collapse in mouse cortical neurons. Thus, we propose that this peptide is a promising lead in finding reagents capable of inhibiting RGMa signaling

The ArcAB two-component regulatory system promotes resistance to reactive oxygen species and systemic infection by Salmonella Typhimurium

Indexación: Scopus.Funding:Thisworkwassupportedby"Fondo NacionaldeCienciayTecnologia"FONDECYT Grants#1120384and#1160315(toCPS),Grant PFB-16(toRP)from"Comisio ´nNacionalde Investigacio ´ n Cientı ´ fica y Tecnolo ´ gica de Chile" (CONICYT)andUniversidadAndresBelloNucleo GrantDI-3-17/N(toCPS),andDoctoralSalmonella enterica Serovar Typhimurium (S. Typhimurium) is an intracellular bacterium that overcomes host immune system barriers for successful infection. The bacterium colonizes the proximal small intestine, penetrates the epithelial layer, and is engulfed by macrophages and neutrophils. Intracellularly, S. Typhimurium encounters highly toxic reactive oxygen species including hydrogen peroxide and hypochlorous acid. The molecular mechanisms of Salmonella resistance to intracellular oxidative stress is not completely understood. The ArcAB two-component system is a global regulatory system that responds to oxygen. In this work, we show that the ArcA response regulator participates in Salmonella adaptation to changing oxygen levels and is also involved in promoting intracellular survival in macrophages and neutrophils, enabling S. Typhimurium to successfully establish a systemic infection. © 2018 Pardo-Esté et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.https://journals.plos.org/plosone/article?id=10.1371/journal.pone.020349

Lawson criterion for ignition exceeded in an inertial fusion experiment

For more than half a century, researchers around the world have been engaged in attempts to achieve fusion ignition as a proof of principle of various fusion concepts. Following the Lawson criterion, an ignited plasma is one where the fusion heating power is high enough to overcome all the physical processes that cool the fusion plasma, creating a positive thermodynamic feedback loop with rapidly increasing temperature. In inertially confined fusion, ignition is a state where the fusion plasma can begin "burn propagation" into surrounding cold fuel, enabling the possibility of high energy gain. While "scientific breakeven" (i.e., unity target gain) has not yet been achieved (here target gain is 0.72, 1.37 MJ of fusion for 1.92 MJ of laser energy), this Letter reports the first controlled fusion experiment, using laser indirect drive, on the National Ignition Facility to produce capsule gain (here 5.8) and reach ignition by nine different formulations of the Lawson criterion

навчальний посібник

Законодавче забезпечення правоохоронної діяльності : навч. посіб. / за заг. ред. В. В. Сокуренка. – Харків : Стильна типографія, 2017. – 1164 с. – (до 100-річчя підготовки охоронців правопорядку у Харкові).У навчальному посібнику представлено методичні рекомендації, пропозиції і зауваження до проектів нормативно-правових актів та наукові роз’яснення окремих положень чинного законодавства, розроблені кафедрами факультету № 1 Харківського національного університету внутрішніх справ. Наукові праці, що включено до навчального посібника, безпосередньо стосуються питань удосконалення форм та методів діяльності слідчих і інших підрозділів органів Національної поліції України. Для працівників органів Національної поліції України, науковців, викладачів, ад’юнктів, аспірантів, докторантів, курсантів, студентів та слухачів навчальних закладів юридичного профілю, а також усіх, хто цікавиться питаннями удосконалення діяльності органів Національної поліції України і інших органів правопорядку.The training manual presents methodological recommendations, suggestions and comments on draft normative legal acts and scientific explanations of certain provisions of the current legislation developed by the departments of the faculty No. 1 of the Kharkov National University of Internal Affairs. Scientific works, included in the textbook, directly relate to issues of improving the forms and methods of activity of investigative and other units of the National Police of Ukraine. For employees of the National Police of Ukraine, scientists, teachers, adjuncts, graduate students, doctoral students, cadets, students and students of legal educational institutions, as well as anyone interested in improving the activities of the National Police of Ukraine and other law enforcement agencies.В учебном пособии представлены методические рекомендации, предложения и замечания к проектам нормативно-правовых актов и научные разъяснения отдельных положений действующего законодательства, разработанные кафедрами факультета № 1 Харьковского национального университета внутренних дел. Научные труды, включен в учебного пособия, непосредственно касаются вопросов совершенствования форм и методов деятельности следственных и других подразделений органов Национальной полиции Украины. Для работников органов Национальной полиции Украины, научных работников, преподавателей, адъюнктов, аспирантов, докторантов, курсантов, студентов и слушателей учебных заведений юридического профиля, а также всех, кто интересуется вопросами совершенствования деятельности органов Национальной полиции Украины и других органов правопорядка