KRONİK OLARAK ATIK ANESTEZİK GAZLARA MARUZ KALAN ANESTEZİ PERSONELİNDE GLUTATİON, TOTAL ANTİOKSİDAN DÜZEYLERİNİN DNA HASARI İLE İLİŞKİSİNİN ARAŞTIRILMASI

rsonelinde Glutation, Total Antioksidan Düzeylerinin DNA Hasarı ile İlişkisinin Araştırılması İnhalasyon anesteziklerinin mutajenik ve genotoksik etkilerinin tartışıldığı araştırma sayısı artmaktadır. IARC inhalasyon anesteziklerini, insanlarda karsinojenitesi sınıflandırılmamış bileşikler (grup III) grubunda değerlendirmektedir. Çalışmamız; DNA hasarının kantitatif olarak ölçüldüğü; kuyruk uzunluğu (TL), kuyruk yoğunluğu (TI), kuyruk momenti (TM), total antioksidan kapasite ve redükte glutation farklılıklarını belirlemek amacıyla ile benzer ameliyathane koşullarında, genellikle nitröz oksit, izofluran, sevofluran ve desfluran gibi inhalasyon anesteziklerine maruz kalan 31 kadın ve 9 erkek anestezi personeli ile yaşları çalışma grubumuzla uyumlu olarak seçilmiş, inhalasyon anesteziklerine maruz kalmayan 40 sağlık çalışanının gönüllü olarak katıldığı kontrol grubundan oluşmuştur. İnhalasyon anesteziklerine maruz kalan grubun periferal kan lenfositlerinde, comet görüntü analiz tekniği ile belirlenen TL, TI, TM parametreleri, kontrol grubu ile kıyaslandığında istatistiksel olarak anlamlı derecede yüksek bulunmuş (p<0,001), sigara içme alışkanlığının bu farklılığı değiştirmediği belirlenmiştir. Ayrıca, maruz kalan grubun total antioksidan kapasitesinin (1,16 ± 0,45), kontrol grubununkinden (0,66 ± 0,31) istatistiksel olarak anlamlı düşük olduğu saptanmıştır (p<0,001). Redükte glutation düzeyleri incelendiğinde ise, kontrol grubunun 1,29 ± 0,29 redükte glutation düzeylerine karşın, inhalasyon anesteziklerine maruz kalan grubun 1,08 ± 0,33 redükte glutation düzeyleri olduğu, kontrol grubununkinden istatistiksel olarak anlamlı olarak düşük olduğu saptanmıştır (p<0,05). Total antioksidan kapasitenin ve redükte glutation düzeyinin sigara içme alışkanlığından etkilenmediği belirlenmiştir. Bu çalışmadan elde edilen sonuçlar, inhalasyon anesteziklerinin mesleki olarak maruz kalan personelde sağlık riski yaratabileceğini göstermektedir.Assessment of association between glutathione levels, total antioxidant capacity and DNA damage in a group of anaesthesia personnel chronically exposed to waste anaesthetic gases There is an increase in the number of studies which discusses the mutagenic and genotoxic effects of inhalation anaesthetics. IARC has considered the carcinogenicity classification of inhalation anaesthetics in the group of 3 (not classifiable as to carcinogenicity to humans). In this study the quantitative measurement of DNA damage; tail length(TL), intensity(TI), and moment(TM), total antioxidant capacity(TAC), and reduced glutathione levels(GSH) of 31 women and 9 men anaesthesia personnel working in similar operating theatre conditions generally exposed to inhalation anaesthetics such as nitrous oxide, isoflurane, sevoflurane, desflurane were measured as well as in an age-matched control group comprised of 40 volunteer healthy workers without occupational exposure to inhalation anaesthetics . &#8220;TL,TI,TM&#8221; parameters determined by the comet image analysing technique in the peripheral blood lymphocytes of the exposed group showed a statistically significant increase comparing to those of the control group(p<0,001), identifying that this disparity did not change in accordance with smoking habits. It was demonstrated that TAC (1,16±0,45) of exposed group was statistical significantly lower than that (0,66±0,31) of the control group. As far as their GSH was concerned, despite the 1,29±0,29 GSH levels of the control group, it was established that the group exposed to inhalation anaesthetics had 1,08±0,33 GSH levels(p<0,05), and was statistical significantly lower than that of control group. It was observed that smoking habit did not have an effect on the TAC and GSH levels. The results achieved in this study reveal that occupational exposure to inhalation anaesthetics could have an impact on health risk

Acute Myopericarditis Mimicking Acute Myocardial Infarction

Acute coronary syndromes among young adults are relatively low when compared with older population in the intensive care unit. Electrocardiographic abnormalities mimicking acute coronary syndromes may be caused by non-coronary syndromes and the differential diagnosis requires a detailed evaluation. We are reporting a case of myopericarditis presenting with acute ST elevation and elevated cardiac enzymes simulating acute coronary syndrome. In this case report, the literature is reviewed to discuss the approach to distinguish an acute coronary syndrome from myopericarditis. (Journal of the Turkish Society Intensive Care 2011; 9:68-70

Can Sevoflurane Induce Micronuclei Formation in Nasal Epithelial Cells of Adult Patients?

Volatile anaesthetics can inhibit the bronchociliary clearence in a dose- and time-dependend way. Moreover, they can have potential mutagenic/carcinogenic effects under chronic exposure. A genotoxicity test -micronuclei assay- was carried out in nasal epithelial cells to analyze the genotoxic effect of sevoflurane in adult patients undergoing general anesthesia

Oxidative DNA damage and total antioxidant status in rats during experimental gram-negative sepsis

Sepsis and septic shock remains as leading cause of death in adult intensive care units. It is widely accepted that gram-negative bacteria and their endotoxins cause sepsis and septic shock, predominantly. Enhanced generation of reactive oxygen species may be responsible for tissue injury in septic shock and endotoxemia. The aim of this study was to assess oxidative DNA damage and the total antioxidant status (TAS) in peripheral lymphocytes of rats during different intraperitoneal gram-negative sepsis stages. Adult male Sprague-Dawley rats were divided randomly into four groups. Control group was intraperitoneally inoculated with 2 ml, of pyrogene-free saline (Group I, n = 6), and the other rats received an intraperitoneal inoculum with 2 ml, of saline containing 2 x 10(8) CFU of Escherichia coli. The animals were killed at time zero (Group 1, n = 6), at 6th (Group 11, n = 7), 12th (Group M, n = 7), and 24th (Group IV, n = 7) hour after the E. coli inoculation. Oxidative DNA damage in peripheral lymphocytes of rats was evaluated by modified comet assay (single-cell gel electrophoresis). Formamido-pyrimidine DNA glycosylase (Fpg) and Endonuclease III (Endo III) were used to detect oxidized purines and pyrimidines, respectively. Total antioxidant quantification was carried out using ABTS+ (2,2'-Azino-di-[3 ethyl-benzthiazoline sulphonate]) radical formation kinetics (Randox kit) in serum samples. Significant elevations of basal levels of strand breaks (SB) in Group IV were observed as compared with Group I, II, and III. There was a significant increase in Fpg sites in Group III as compared with Group I and II. However, there was no significant difference in terms of Endo III sites in any of the groups. Although the TAS was decreased with the stages of sepsis, this moderate decrease was significant in only Group IV as compared with Group I. There was no statistically significant correlation between DNA damage and TAS for any of the groups