Are Induced Pluripotent Stem Cells a Step towards Modeling Pediatric Leukemias?

Despite enormous improvements in pre-clinical and clinical research, acute leukemia still represents an open challenge for pediatric hematologists; both for a significant relapse rate and for long term therapy-related sequelae. In this context, the use of an innovative technology, such as induced pluripotent stem cells (iPSCs), allows to finely reproduce the primary features of the malignancy and can be exploited as a model to study the onset and development of leukemia in vitro. The aim of this review is to explore the recent literature describing iPSCs as a key tool to study different types of hematological malignancies, comprising acute myeloid leukemia, non-down syndrome acute megakaryoblastic leukemia, B cell acute lymphoblastic leukemia, and juvenile myelomonocytic leukemia. This model demonstrates a positive impact on pediatric hematological diseases, especially in those affecting infants whose onsets is found in fetal hematopoiesis. This evidence highlights the importance of achieving an in vitro representation of the human embryonic hematopoietic development and timing-specific modifications, either genetic or epigenetic. Moreover, further insights into clonal evolution studies shed light in the way of a new precision medicine era, where patient-oriented decisions and therapies could further improve the outcome of pediatric cases. Nonetheless, we will also discuss here the difficulties and limitations of this model

Cross-mapping the Gothic

Cross-mapping the Gothi

La gestione degli acquisti. Strategia, implementazione, controllo

Il manuale ha l'obiettivo di fornire al manager degli acquisti una guida completa su tutti gli aspetti che caratterizzano la sua funzione, ai differenti livelli di responsabilit\ue0, dalla base al vertice e in contesti aziendali articolati. Un'attenzione particolare \ue8 rivolta al processo di acquisto, nonch\ue9 alle procedure che sottendono alle diverse attivit\ue0, con esempi applicabili all'interno dei diversi tipi di azienda, a seconda che venga esercitato un controllo pi\uf9 o meno centralizzato. Si spazia dal vendor rating all'analisi del valore; dal redesign to cost agli strumenti informatici; da come affrontare in maniera idonea una negoziazione a come misurare la performance in termini di KPI: tutti temi che devono far parte del bagaglio culturale di ogni buyer, insieme alla valutazione della qualit\ue0 dei fornitori e agli aspetti legali del rapporto di fornitura. Il taglio fortemente pratico del testo permette di trattare con il dovuto approfondimento anche gli aspetti giuridici legati a questa attivit\ue0, che se da una parte costituiscono un vincolo dall'altra sono spesso uno strumento fondamentale nella relazione con il fornitore. Inoltre anche i temi pi\uf9 teorici legati alla sfera economica finanziaria e al TCO o quelli inerenti la strategia e la stesura del piano degli acquisti, sono sempre affrontati in un'ottica molto pragmatica e finalizzata all'applicazione. La preparazione degli autori e la completezza della trattazione fanno di quest'opera un manuale essenziale per tutte le esigenze di una professione sempre pi\uf9 complessa e in cui l'orizzonte delle competenze necessarie si amplia continuamente in ragione di un mercato dinamico, globale e imprevedibile

The effect of irrigation on fruit development of olive cultivars 'Frantoio' and 'Leccino'

A field study was conducted on mature trees of two olive cultivars (`Frantoio' and `Leccino') in Tuscany (Italy) to assess differences between irrigated and rainfed trees in fruit growth parameters and the cellular processes determining mesocarp growth. Reference evapotranspiration, calculated by the Penman-FAO equation, was multiplied by a crop coefficient of 0.55 or 0.65 to determine the amount of irrigation water. The fresh weight and dry weight of fruits and their tissues were monitored at approximate two-week intervals. At 9 (end of massive endocarp sclerification) and 20 weeks after full bloom (AFB) fruit anatomical parameters were measured in transverse equatorial sections using image analysis. Fruits from irrigated trees showed greater equatorial diameter and mesocarp transverse area than those from rainfed trees of both cultivars at 9 and 20 weeks AFB. Irrigation significantly increased the size of mesocarp cells of both cultivars at 9 and 20 weeks AFB, and cell number in `Leccino' at 9 weeks AFB

The effect of irrigation on fruit development of olive cultivars 'frantoio' and 'leccino'

Trabajo presentado en el IV International Symposium on Irrigation of Horticultural Crops, celebrado en Davis (Estados Unidos) el 01 de septiembre de 2003.A field study was conducted on mature trees of two olive cultivars (‘Frantoio’ and ‘Leccino’) in Tuscany (Italy) to assess differences between irrigated and rainfed trees in fruit growth parameters and the cellular processes determining mesocarp growth. Reference evapotranspiration, calculated by the Penman-FAO equation, was multiplied by a crop coefficient of 0.55 or 0.65 to determine the amount of irrigation water. The fresh weight and dry weight of fruits and their tissues were monitored at approximate two-week intervals. At 9 (end of massive endocarp sclerification) and 20 weeks after full bloom (AFB) fruit anatomical parameters were measured in transverse equatorial sections using image analysis. Fruits from irrigated trees showed greater equatorial diameter and mesocarp transverse area than those from rainfed trees of both cultivars at 9 and 20 weeks AFB. Irrigation significantly increased the size of mesocarp cells of both cultivars at 9 and 20 weeks AFB, and cell number in ‘Leccino’ at 9 weeks AFB.Peer reviewe

Trisomy 11 with MLL-PTD in a case of infant AML M0

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