Fiscally responsible mafia clans

To work as an accountability mechanism, fiscal decentralization requires the democratic mechanism to function well. This is generally not the case where the quality of the institutional environment is weak, and local governments might be captured by local oligarchs. We explore this issue by studying how Italian municipalities reacted to an unexpected tax reform reducing tax autonomy at the local level. Focusing on three Southern provinces, where some municipal governments are captured by Camorra clans, our estimates suggest that captured municipalities are less responsive to incentives stemming from recentralization. We also observe a different recomposition of spending between the two groups, which we interpret – according to our theoretical model – considering the different degrees with which clans can extract rents from public expenditure

Taxonomic notes on Lyroglossa and Pteroglossa (Orchidaceae: Spiranthinae) : two new generic records for the flora of Rio Grande do Sul

Lyroglossa and Pteroglossa (Orchidaceae: Spiranthinae) are first recorded for Rio Grande do Sul. Descriptions, illustrations, taxonomic notes and distribution are presented. We also provide an artificial key to distinguish the genera of the “Stenorrhynchos Clade” reported so far in Rio Grande do Sul

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY A N I N T E R N A T I O N A L J O U R N A L Late Holocene Vegetation History and Early Evidence of Araucaria angustifolia in Caçapava do Sul in the Lowland Region of Rio Grande do Sul State, Southern Brazil

ABSTRACT Little is known about the southernmost occurrence of small areas with Araucaria angustifolia populations in Caçapava do Sul in low elevated areas of Rio Grande d

Performance of 'Emerald' and 'Jewel' blueberry cultivars under no-chill incidence

Climate is a limiting factor for cultivating blueberries in new areas in Brazil, being the commercial production restricted only to the colder and high-altitude regions of the southern states. This study aimed to evaluate the performance of low-chill requirement 'Emerald' and 'Jewel' blueberry cultivars. For that, the stages of vegetative growth, sprouting, flowering fruiting and yield were assessed. The 'Emerald' cultivar has two peaks for both flowering and fruiting, with the main harvest period occurring between August and October, while the 'Jewel' cultivar shows two peaks for budding, but only one flowering peak and only one harvest, which extends from October through January. For both cultivars, the production occurs during the off-season of the internal and external markets, resulting in higher fruit prices. Growing both cultivars is feasible in a subtropical region with no-chill incidence, thus demonstrating that these cultivars have much lower chill requirements than those referred to in the literature, and favoring, this way, the fresh fruit production during the off-season of the main producing countries in the northern hemisphere

Estudo da atividade antiviral do óleo essencial de Glechon spathulata Benth sobre o herpes simples virus tipo I (HSV-1)

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130 purification of large scale mrna encoding zfn nucleases by dhplc technology

A novel strategy of targeted gene correction of the interleukin-2 receptor common gamma chain (IL2RG) gene for the treatment of X-linked Severe Combined Immunodeficiency (SCID-X1) is achieved by the combination of a pair of IL2RG-specific Zinc Finger Nucleases (ZFN) and the correct-gene template DNA delivered by integration-defective lentiviral vector (IDLV).The transient expression of the ZFN pair targeting the disease-causing gene is obtained by the electroporation of the two corresponding mRNAs, produced by in vitro transcription starting from plasmid DNA template. A major limitation of the mRNA transcribed in vitro is the presence of residual contaminants such as short RNAs and double stranded (ds)RNAs that may affect the function and spectrophotometric quantification of the product hampering therefore the delivery of high quality and precise amount of mRNA to target cells. Moreover, dsRNA contaminants represent a possible risk in terms of immunogenicity of the product, leading to activation of unwanted innate immune response with consequent reduction/abrogation of mRNA translation as well as potential alteration of the properties of the transfected cells. To improve nuclease expression while decreasing cellular innate response to mRNA transfection we combined different strategies: (i) inclusion of UTRs and polyA tails in the DNA template used for mRNA production; (ii) use of modified nucleotides during mRNA production and (iii) purification of the mRNAs by dHPLC with a reverse phase column made of non-porous matrix consisting of polystyrene-divinylbenzene copolymer beads alkylated with C-18 chains (Transgenomic, LTD.). In particular, the purification of in vitro transcribed mRNAs by means of dHPLC has been shown to strongly improve the translation of mRNA and significantly reduce the contaminant presence thus preventing innate immunity and eventually increasing modified cells persistence in vivo. We have developed feasible and reproducible, small and large scale mRNA production and downstream purification processes of the ZFN pairs obtaining accurate RNA quantification and reduced risk of immunogenicity. The full process achieved a 60% yield, loading with a 500µg RNA for each run with a single clean chromatographic peak. Furthermore, the level of residual organic solvent (i.e. Acetonitrile) used in the purification process is compatible with that applicable into clinic. The highly translatable non-immunogenic dHPLC-purified mRNA can be delivered without toxicity and represents a powerful and safe tool for the application of gene therapy protocols

Análise química de Hypericum carinatum (guttiferae)

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Correction of metachromatic leukodystrophy in the mouse model by transplantation of genetically modified hematopoietic stem cells

Gene-based delivery can establish a sustained supply of therapeutic proteins within the nervous system. For diseases characterized by extensive CNS and peripheral nervous system (PNS) involvement, widespread distribution of the exogenous gene may be required, a challenge to in vivo gene transfer strategies. Here, using lentiviral vectors (LVs), we efficiently transduced hematopoietic stem cells (HSCs) ex vivo and evaluated the potential of their progeny to target therapeutic genes to the CNS and PNS of transplanted mice and correct a neurodegenerative disorder, metachromatic leukodystrophy (MLD). We proved extensive repopulation of CNS microglia and PNS endoneurial macrophages by transgene-expressing cells. Intriguingly, recruitment of these HSC-derived cells was faster and more robust in MLD mice. By transplanting HSCs transduced with the arylsulfatase A gene, we fully reconstituted enzyme activity in the hematopoietic system of MLD mice and prevented the development of motor conduction impairment, learning and coordination deficits, and neuropathological abnormalities typical of the disease. Remarkably, ex vivo gene therapy had a significantly higher therapeutic impact than WT HSC transplantation, indicating a critical role for enzyme overexpression in the HSC progeny. These results indicate that transplantation of LV-transduced autologous HSCs represents a potentially efficacious therapeutic strategy for MLD and possibly other neurodegenerative disorders