3 research outputs found

    Déterminisme génétique de la tolérance de Lagenaria siceraria (Molina) Standley (Cucurbitaceae) aux insectes ravageurs et recherche de marqueurs microsatellites SSR associés aux gènes

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    La gourde olĂ©agineuse, Lagenaria siceraria (Molina) Standley, se caractĂ©rise par un haut potentiel nutritionnel et une forte valeur marchande dont l’intensification de la production pourrait profiter aux agriculteurs, en particulier, les femmes rurales qui en sont les principales productrices. MalgrĂ© l’importance socio-Ă©conomique de la gourde olĂ©agineuse, la production est caractĂ©risĂ©e par de faibles rendements, dus essentiellement aux dĂ©gâts occasionnĂ©s par les insectes ravageurs. La prĂ©sente Ă©tude vise Ă  connaitre le dĂ©terminisme gĂ©nĂ©tique de la tolĂ©rance de Lagenaria siceraria aux insectes ravageurs et Ă  identifier les marqueurs molĂ©culaires proches des QTLs contrĂ´lant cette tolĂ©rance. Cette identification serait une Ă©tape importante pour accĂ©lĂ©rer le processus de la tolĂ©rance de L. siceraria aux insectes ravageurs. Des marqueurs molĂ©culaires de type SSR ont Ă©tĂ© Ă©valuĂ©s sur 100 individus de la population F2 (issue de l’autofĂ©condation de la F1 provenant du croisement entre les accessions parentales NI431 et NI227). Un seul QTL dĂ©nommĂ© qTDco a Ă©tĂ© identifiĂ© et le marqueur C1.2.23 a Ă©tĂ© associĂ© Ă  ce QTL. Ce marqueur, utilisĂ©, dans un programme de sĂ©lection assistĂ©e par marqueur, serait un atout considĂ©rable dans le processus de la recherche de la tolĂ©rance de L. siceraria aux insectes ravageurs.   Lagenaria siceraria (Molina) Standley is characterized by high nutritional potential and high market value. The intensification of L. siceraria could benefit farmers, especially rural women who are the main farmers. Despite the socio-economic importance of this oil gourd, its production is characterized by low yields, mainly due to damage caused by insect pests. The present study aims to identify molecular markers SSR associated to QTLs controlling the tolerance of insect damage. Thoses molecular markers would be an important step in accelerating the process of tolerance of L. siceraria to insect pests. Molecular markers microsatellites SSR were evaluated on 100 individuals of the F2 population (resulting from the self-fertilization of F1 from the crossing between parental accessions NI431 and NI227). A single QTL called qTDco was identified and the marker C1.2.23 was associated with this QTL. This marker used in a marker-assisted selection program would be a considerable asset in the process of researching the tolerance of L. siceraria to insect pests

    The mitochondrial genome of an important edible insect species, the African palm weevil (Rhynchophorus phoenicis)

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    The African palm weevil (Rhynchophorus phoenicis) is a species of high economic importance in sub-Saharan Africa, both as a culturally traditional edible insect and as an agricultural pest. Here we provide a de novo assembly and annotation for the mitochondrial genome of this species from whole-genome sequence data. The mitogenome was AT-rich and 17,161bp in length, containing 13 protein-coding, 22 transfer RNA, and two ribosomal RNA genes. Phylogenetic reconstruction showed the African palm weevil to cluster within the genus Rhynchophorus and the weevil tribe Rhynchophorini. This mitogenome will be important for future genetic research into this emerging edible insect species
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