Insights into the molecular phylogeny and morphology of three novel Dothiora species, along with a worldwide checklist of Dothiora

Most species of Dothiora are known from the dead parts of various host plants as saprobic fungi in terrestrial habitats occurring in tropical and temperate regions. In the present study, samples of Dothiora were collected from dead twigs and branches of Capparis spinosa, Rhaponticum repens, and an unknown angiosperm plant from the Tashkent and Jizzakh regions of Uzbekistan. Multi-gene phylogenetic analyses based on a combined ITS, LSU, SSU, TEF1, and TUB2 sequence data revealed their taxonomic positions within the Dothideaceae. Three new species of Dothiora, namely, Dothiora capparis, Dothiora rhapontici, and Dothiora uzbekistanica were proposed by molecular and morphological data. Likewise, the phylogenetic relationship and morphology of Dothiora are discussed. In addition, we provide a list of accepted Dothiora species, including host information, distribution, morphology descriptions, and availability of sequence data, to enhance the current knowledge of the diversity within Dothiora

Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi

This article is the ninth in the series of Fungal Diversity Notes, where 107 taxa distributed in three phyla, nine classes, 31 orders and 57 families are described and illustrated. Taxa described in the present study include 12 new genera, 74 new species, three new combinations, two reference specimens, a re-circumscription of the epitype, and 15 records of sexualasexual morph connections, new hosts and new geographical distributions. Twelve new genera comprise Brunneofusispora, Brunneomurispora, Liua, Lonicericola, Neoeutypella, Paratrimmatostroma, Parazalerion, Proliferophorum, Pseudoastrosphaeriellopsis, Septomelanconiella, Velebitea and Vicosamyces. Seventy-four new species are Agaricus memnonius, A. langensis, Aleurodiscus patagonicus, Amanita flavoalba, A. subtropicana, Amphisphaeria mangrovei, Baorangia major, Bartalinia kunmingensis, Brunneofusispora sinensis, Brunneomurispora lonicerae, Capronia camelliaeyunnanensis, Clavulina thindii, Coniochaeta simbalensis, Conlarium thailandense, Coprinus trigonosporus, Liua muriformis, Cyphellophora filicis, Cytospora ulmicola, Dacrymyces invisibilis, Dictyocheirospora metroxylonis, Distoseptispora thysanolaenae, Emericellopsis koreana, Galiicola baoshanensis, Hygrocybe lucida, Hypoxylon teeravasati, Hyweljonesia indica, Keissleriella caraganae, Lactarius olivaceopallidus, Lactifluus midnapurensis, Lembosia brigadeirensis, Leptosphaeria urticae, Lonicericola hyaloseptispora, Lophiotrema mucilaginosis, Marasmiellus bicoloripes, Marasmius indojasminodorus, Micropeltis phetchaburiensis, Mucor orantomantidis, Murilentithecium lonicerae, Neobambusicola brunnea, Neoeutypella baoshanensis, Neoroussoella heveae, Neosetophoma lonicerae, Ophiobolus malleolus, Parabambusicola thysanolaenae, Paratrimmatostroma kunmingensis, Parazalerion indica, Penicillium dokdoense, Peroneutypa mangrovei, Phaeosphaeria cycadis, Phanerochaete australosanguinea, Plectosphaerella kunmingensis, Plenodomus artemisiae, P. lijiangensis, Proliferophorum thailandicum, Pseudoastrosphaeriellopsis kaveriana, Pseudohelicomyces menglunicus, Pseudoplagiostoma mangiferae, Robillarda mangiferae, Roussoella elaeicola, Russula choptae, R. uttarakhandia, Septomelanconiella thailandica, Spencermartinsia acericola, Sphaerellopsis isthmospora, Thozetella lithocarpi, Trechispora echinospora, Tremellochaete atlantica, Trichoderma koreanum, T. pinicola, T. rugulosum, Velebitea chrysotexta, Vicosamyces venturisporus, Wojnowiciella kunmingensis and Zopfiella indica. Three new combinations are Baorangia rufomaculata, Lanmaoa pallidorosea and Wojnowiciella rosicola. The reference specimens of Canalisporium kenyense and Tamsiniella labiosa are designated. The epitype of Sarcopeziza sicula is re-circumscribed based on cyto- and histochemical analyses. The sexual-asexual morph connection of Plenodomus sinensis is reported from ferns and Cirsium for the first time. In addition, the new host records and country records are Amanita altipes, A. melleialba, Amarenomyces dactylidis, Chaetosphaeria panamensis, Coniella vitis, Coprinopsis kubickae, Dothiorella sarmentorum, Leptobacillium leptobactrum var. calidus, Muyocopron lithocarpi, Neoroussoella solani, Periconia cortaderiae, Phragmocamarosporium hederae, Sphaerellopsis paraphysata and Sphaeropsis eucalypticola

Readerielliopsidaceae Abdollahz. & Crous 2020

Key to genera of Readerielliopsidaceae 1. Produce a thin superficial network of dense, dark hyphae on leaves.................................................................................................2 - Produce hyphal mass, black, web-like colonies on leaves.................................................................................................................5 2. Pycnidia flask shaped or sub globose to pyriform, brown.................................................................................................................3 - Pycnidia irregularly cylindrical, straight or flexuous, short or long........................................................................... Scolecoxyphium 3. Conidia globose to clavate, non-septate..................................................................................................................... Readerielliopsis - Conidia with septate...........................................................................................................................................................................4 4. Conidia filiform, fusoid-ellipsoidal, with up to 15-septate......................................................................................... Phaeoxyphiella - Conidia oblong or ovoid, with 3–4 transverse septa..................................................................................................... Fumagospora 5. Ascostromata with stalks, ascospore oblong to saccate, 3-septate, hyaline............................................................................. Scorias - Ascostromata without stalks, ascospore fusoid, 3-septate with a mucilaginous sheath, hyaline...................................... AlloscoriasPublished as part of Haituk, Sukanya, Suwannarach, Nakarin, Hongsanan, Sinang, Senwanna, Chanokned & Cheewangkoon, Ratchadawan, 2021, New genus of epiphytic sooty mold: Alloscorias syngonii (Readerielliopsidaceae) from Thailand, pp. 271-282 in Phytotaxa 507 (4) on page 276, DOI: 10.11646/phytotaxa.507.4.1, http://zenodo.org/record/542569

Coryneum heveanum sp. nov. (Coryneaceae, Diaporthales) on twigs of Para rubber in Thailand

During studies of microfungi on para rubber in Thailand, we collected a new Coryneum species on twigs which we introduce herein as C. heveanum with support from phylogenetic analyses of LSU, ITS and TEF1 sequence data and morphological characters. Coryneum heveanum is distinct from other known taxa by its conidial measurements, number of pseudosepta and lack of a hyaline tip to the apical cell

Anteaglonium saxicola N. Suwannarach & J. Kumla 2023, sp. nov.

<i>Anteaglonium saxicola</i> N. Suwannarach & J. Kumla <i>sp. nov.</i> FIGURE 2 <p>MycoBank number: MB850037</p> <p> Etymology:— ‘ <i>saxicola</i> ’ refers to a stone inhabitant.</p> <p>Type:— THAILAND, Lamphun Province, Mueang Lamphun District, Sribuaban Subdistrict, Chiang Mai University Hariphunchai Campus, 18°32′11′′N, 99°07′30′′ E, isolated from a rock of a natural forest, 15 November 2022, N. Suwannarach, SDBR-CMU481, a dried cultured on PDA, culture ex-type SDBR-CMU481, preserved in a metabolically inactive state.</p> <p> Description:—Isolated from rock in terrestrial habitat. Sporulation on PDA after incubation at 25°C for two months. <i>Conidiomata</i> were observed under a stereo microscope, 70–175 µm high, 60–145 µm diam., pycnidial, globose to subglobose, scattered or aggregated into clusters, immersed to erumpent, dark brown to black, ostiolate, covered with white mycelia. <i>Conidiomata wall</i> 8–44 µm wide, composed of thick-walled cells of <i>textura angularis</i>, brown to dark brown. <i>Conidiophores</i> reduced to conidiogenous cells. <i>Conidiogenous cells</i> 8–19 × 2–6 µm, hyaline, smooth, ampulliform to subcylindrical, arising from the inner cavity of the conidioma wall. <i>Conidia</i> 3–4 × 2–3 µm, hyaline, solitary, subglobose to broadly ellipsoid, aseptate, smooth-walled.</p> <p>Culture characteristics:—Colonies on PDA reaching 32–35 mm diam. after 4 weeks at 25°C, flattened, wrinkled, with entire edges; colony from above, greyish-white at the center, greyish red at the margin, produced a soluble red pigment; from below: greyish ruby at the center, dark ruby at the margin. Colonies on MEA reaching 23–26 mm diam. after 4 weeks at 25°C, flattened, with entire edges, slightly concentric ring in the middle; colony from above, greyish-white; from below: brown, with white at the margin, dark grey in the middle, radiated with concentric sectors, producing light brown pigmentation around the colony. Colonies on CMD reaching 32–35 mm diam. after 4 weeks at 25°C, flattened, with entire edges, concentric ring; colony from above, light brown in the center, yellowish-white at the margin; from below: light brown in the middle, with white to cream at the margin, strongly radiated with concentric sectors, producing light brown pigmentation around the colony.</p> <p>Additional specimen examined:— THAILAND, Lamphun Province, Mueang Lamphun District, Sribuaban Subdistrict, Chiang Mai University Hariphunchai Campus, 18°32′11′′N, 99°07′30′′ E, isolated from a rock of a natural forest, 15 November 2022, N. Suwannarach, SDBR-CMU482, a dried cultured on PDA, living culture SDBR-CMU482.</p> <p> Notes:—Morphologically, the new species fitted well with the asexual morph of the genus <i>Anteaglonium</i>. The growth of <i>A. saxicola</i> at 25°C on MEA was slower than <i>A. parvulum</i> and <i>A. thailandicum</i> (13–16 and 23 mm, respectively, after incubation for 7 d) (Jayasiri <i>et al.</i> 2016), while it displayed faster growth than <i>A. rubescens</i> (11–13 mm after incubation for 2 months) (Jaklitsch <i>et al.</i> 2018). Remarkedly, <i>A. saxicola</i> displayed faster growth than <i>A. rubescens</i> on PDA and CMD (10 and 5–6 mm, respectively) after incubation at 25°C for four weeks. Based on the asexual morph characteristics, <i>A. saxicola</i> differs from <i>A. parvulum</i>, <i>A. rubescens</i>, and <i>A. thailandicum</i> by its longer conidiogenous cells (TABLE 2) (Jayasiri <i>et al.</i> 2016, Jaklitsch <i>et al.</i> 2018). Moreover, <i>A. saxicola</i> can be distinguished from <i>A. rubescens</i> by its wider conidiomata wall (Jaklitsch <i>et al.</i> 2018). However, due to the indeterminate morphological characteristics of the asexual morphs of <i>A. abbreviatum</i>, <i>A. brasiliense</i>, <i>A. globosum</i>, <i>A. gordoniae</i>, <i>A. latirostrum</i>, <i>A. lusitanicum</i>, and <i>A. queenslandicum</i>, we cannot compare their microscopic structures (Mugambi & Huhndorf 2009, Almeida <i>et al.</i> 2014, Jayasiri <i>et al.</i> 2019, Tan <i>et al.</i> 2022, Tan & Shivas 2023).</p> <p> Phylogenetically, <i>Anteaglonium saxicola</i> is closely related to <i>A</i>. <i>lusitanicum</i> and <i>A. parvulum</i>; however, the genetic distance of the nrLSU and ITS sequences of <i>A. saxicola</i> to <i>A. lusitanicum</i> was 0.93 and 5.5% (8/867 and 27/491 bp including gaps), respectively. Furthermore, the genetic distance of nrLSU and ITS sequences also indicated that <i>A. saxicola</i> differs from <i>A. parvulum</i> MFLUCC 10-0928, MFLUCC 11-0511, MFLUCC 11-0374, and MFLUCC 11-0380 by having 1.35% and 6.97% (11/817 and 34/488 bp including gaps), 1.24% and 5.92% (10/805 and 29/490 bp including gaps), 1.24% and 6.31% (10/804 and 31/491 bp include gaps), and 1.49% and 6.12% (12/804 and 30/490 bp including gaps), respectively.</p>Published as part of <i>Suwannarach, Nakarin, Kumla, Jaturong, Khuna, Surapong, Thitla, Tanapol, Senwanna, Chanokned, Hongsanan, Sinang & Lumyong, Saisamorn, 2023, Anteaglonium saxicola (Anteagloniaceae, Pleosporales), a new species isolated from rocks in northern Thailand, pp. 75-84 in Phytotaxa 629 (1)</i> on pages 79-81, DOI: 10.11646/phytotaxa.629.1.6, <a href="http://zenodo.org/record/10254797">http://zenodo.org/record/10254797</a&gt

FIGURE 1 in Anteaglonium saxicola (Anteagloniaceae, Pleosporales), a new species isolated from rocks in northern Thailand

FIGURE 1. Phylogram derived from maximum likelihood analysis of a combined nrLSU, nrSSU, ITS, and tef1-α genes of 26 sequences. Bootstrap values ≥ 75% ML (left) and Bayesian posterior probabilities ≥ 0.90 (right) are shown above nodes. The scale bar represents the expected number of nucleotide substitutions per site. Sequence data obtained from this study are in red. Ex-type strains are in bold.Published as part of <i>Suwannarach, Nakarin, Kumla, Jaturong, Khuna, Surapong, Thitla, Tanapol, Senwanna, Chanokned, Hongsanan, Sinang & Lumyong, Saisamorn, 2023, Anteaglonium saxicola (Anteagloniaceae, Pleosporales), a new species isolated from rocks in northern Thailand, pp. 75-84 in Phytotaxa 629 (1)</i> on page 79, DOI: 10.11646/phytotaxa.629.1.6, <a href="http://zenodo.org/record/10254797">http://zenodo.org/record/10254797</a&gt

Multi-Gene Phylogeny and Morphology Reveal Haplohelminthosporium gen. nov. and Helminthosporiella gen. nov. Associated with Palms in Thailand and A Checklist for Helminthosporium Reported Worldwide

Palms (Arecaceae) are substrates for a highly diverse range of fungi. Many species are known as saprobes and many are important plant pathogens. Over the course of our studies of micro-fungi from palms in Thailand, two new taxa were discovered. Morphological characteristics and phylogenetic analyses of combined ITS, LSU, SSU, and tef1-α sequence data revealed their taxonomic positions within Massarinaceae. There are currently ten genera identified and accepted in Massarinaceae, with the addition of the two new genera of Haplohelminthosporium and Helminthosporiella, that are introduced in this paper. Each new genus is provided with a full description and notes, and each new taxon is provided with an illustration for the holotype. A list of identified and accepted species of Helminthosporium with morphology, host information, locality, sequence data, and related references of Helminthosporium reported worldwide is provided based on records in Species Fungorum 2021. This work provides a micro-fungi database of Haplohelminthosporium, Helminthosporiella, and Helminthosporium which can be modified and validated as new data come to light

Identification and Pathogenicity of Paramyrothecium Species Associated with Leaf Spot Disease in Northern Thailand

Species of Paramyrothecium that are reported as plant pathogens and cause leaf spot or leaf blight have been reported on many commercial crops worldwide. In 2019, during a survey of fungi causing leaf spots on plants in Chiang Mai and Mae Hong Son provinces, northern Thailand, 16 isolates from 14 host species across nine plant families were collected. A new species Paramyrothecium vignicola sp. nov. was identified based on morphology and concatenated (ITS, cmdA, rpb2, and tub2) phylogeny. Further, P. breviseta and P. foliicola represented novel geographic records to Thailand, while P. eichhorniae represented a novel host record (Psophocarpus sp., Centrosema sp., Aristolochia sp.). These species were confirmed to be the causal agents of the leaf spot disease through pathogenicity assay. Furthermore, cross pathogenicity tests on Coffea arabica L., Commelina benghalensis L., Glycine max (L.) Merr., and Dieffenbachia seguine (Jacq.) Schott revealed multiple host ranges for these pathogens. Further research is required into the host–pathogen relationship of Paramyrothecium species that cause leaf spot and their management. Biotic and abiotic stresses caused by climate change may affect plant health and disease susceptibility. Hence, proper identification and monitoring of fungal communities in the environment are important to understand emerging diseases and for implementation of disease management strategies

One stop shop IV: taxonomic update with molecular phylogeny for important phytopathogenic genera: 76–100 (2020)

This is a continuation of a series focused on providing a stable platform for the taxonomy of phytopathogenic fungi and fungus-like organisms. This paper focuses on one family: Erysiphaceae and 24 phytopathogenic genera: Armillaria, Barriopsis, Cercospora, Cladosporium, Clinoconidium, Colletotrichum, Cylindrocladiella, Dothidotthia,, Fomitopsis, Ganoderma, Golovinomyces, Heterobasidium, Meliola, Mucor, Neoerysiphe, Nothophoma, Phellinus, Phytophthora, Pseudoseptoria, Pythium, Rhizopus, Stemphylium, Thyrostroma and Wojnowiciella. Each genus is provided with a taxonomic background, distribution, hosts, disease symptoms, and updated backbone trees. Species confirmed with pathogenicity studies are denoted when data are available. Six of the genera are updated from previous entries as many new species have been described.National Natural Science Foundation of Chin