Ultra Structural Analysis and Lipid Staining of Biodiesel Producing Microalgae - Chlorella vulgaris Collected from Various Ponds in Tamil Nadu, India

The present research paper describes to evaluate 10 different Microalgal lipid contents by Nile red and Bodipy staining. The ultra structural studies carried out by Scanning Electron Microscope (SEM). The micro algal samples were collected from province of Tamil Nadu, located in Southern India.  Compare to Nile Red stain Bodipy is a specific stain for to detect lipid content present in the living algal cells. In this study clearly describe Chlorella vulgaris stained by Bodipy have shown more lipid content than other microalgae collected from various location

A NEW VALIDATED CHIRAL RP-HPLC METHOD FOR THE DETERMINATION OF STABILITY OF MEBEVERINE ENANTIOMERS IN THE PRESENCE OF ITS DEGRADATION PRODUCTS

Objective: An enantioselective reverse phase high performance liquid chromatographic method was developed and validated for the analysis of mebeverine enantiomers. This method was used to investigate the stability and degradation behaviour of mebeverine under different stress conditions recommended by International Conference on Harmonization (ICH).Methods: An Isocratic chiral RP-HPLC method for the resolution of mebeverine and its degradation products was successfully achieved on a Phenomenex® lux cellulose 1 column, using UV detector at wavelength of 219 nm, with a mobile phase consisting of 0.1% diethyl amine in methanol, 20 mM ammonium bicarbonate (pH: 4.6) adjusted with trifluroacetic acid, 0.1% diethyl amine in isopropyl alcohol (55: 15: 30 v/v/v), and a flow rate of 1.2 ml/min. The drug was subjected to alkaline, acidic, neutral, oxidative, and photolytic conditions in order to mimic stress conditions. The stressed samples were also analysed by this method.Results: The method developed provided the linear correlation (R2 =0.999) for the drug between a range of 56–84 µg/ml for (-) mebeverine and 52–78 µg/ml (+) mebeverine respectively. The limit of detection and limit of quantification of (-) mebeverine was found to be 0.30 µg/ml, 0.90 µg/ml and (+) mebeverine was found to be 0.32 µg/ml, 0.97 µg/ml respectively.Conclusion: The method developed was found to provides good sensitivity and excellent precision and reproducibility and can be applied in the quality control of drug products.Â

Birth weight and term of the gestation in pregnancies complicated by isolated oligo and isolated polyhydramnios

Background: To access the birth weight of the newborn and term of the gestation in pregnancies complicated by isolated oligo and polyhydramnios.Methods: A Hospital based study has been conducted in the Department of Obstetrics and Gynaecology, Sri Ramachandra Medical College and Research Institute, Chennai.  All the singleton pregnancies diagnosed with Isolated Oligo and Polyhydramnios were enrolled in the study. All the subjects were followed up to their delivery. Birth weight and Term of the Gestation were recorded immediately after delivery.Results: Out of 3,567 patients 32 (0.9%) and 5 (0.15%) were diagnosed as Isolated Oligo and Polyhydramnios respectively.Among 32 (0.9%) newborns of Isolated Oligohydramnios, 5 (15.62%) were Very Low Birth Weight ( 3800gms). On the other hand Preterm and Term gestations were 17 (53.12%) and 15(46.8%) respectively in this group.Among the 5 (0.15%) cases of Isolated Polyhydramnios group, Very Low Birth Weight and Low Birth Weight newborns were 1(20%) each and newborns with normal Birth weight were 3 (60%). Similarly, Preterm and Term gestations were 2(40%) and 3(60%) respectively.Conclusion:  Isolated Oligo and Polyhydramnios are associated with increased rate of Low Birth Weight (Very Low Birth Weight and Low Birth Weight) neonates and Preterm deliveries.

Design and implementation of FPGA-based high power LED lighting system for ships

724-729Light emitting drive system used in ships induce less input side power factor with highly distorted line (AC) current which brings down the quality of AC power supply system. To suppress these limitations, futuristic ANFIS tuned PI controller operated modified SEPIC rectifier is designed and the effective analysis and results are presented in this paper. More desirable power quality is achieved with ANFIS tuned PI controller when compared with PI/fuzzy tuned PI controller in ship’s LED light module. LED driver module of 48 V, 100 W power rating is built and its upgraded operating performance is accomplished by the execution of controller in FPGA Spartan–6 platform. The satisfactory result of 2.927%-line current harmonic distortion and supply side 0.9991 power factor (within IEEE-516 standard) is attained. Steady state study involving 0.09 s, rise time with zero overshoot is acquired

ENHANCED PRODUCTION OF PSORALEN THROUGH ELICITORS TREATMENT IN ADVENTITIOUS ROOT CULTURE OF PSORALEA CORYLIFOLIA L.

Objective: The present study aimed to determine the effect of two elicitors namely Methyl Jasmonate (MeJ) and Salicylic acid (SA) on adventitious root production of Psoralea corylifolia L. as the elicitors and at different concentrations.Methods: Adventitious roots of P. corylifolia L. were treated with different concentration of elicitors such as MeJ (10, 20, 30 and 40 µM/l) and SA (50, 100, 150 and 200 µM/l) to enhance the psoralen contents. After the treatment, psoralen content was analyzed through the analytical HPLC experiments (Methanol: Water (50:50) at 0.8 ml/min-1 flow rate and the injection volume as 20 µl).Results: MeJ and SA treatments at the concentrations of 30 µM/l and 150 µM/l respectively were found to increase the net wet weight of adventitious root production to 8 fold at 8 hours of elicitor treatment periods. Further, the quantity of psoralen was determined from the elicitors (MeJ and SA) treated roots and mother plant through the analytical HPLC experiments in order to estimate the psoralen content and it was found as 3.73 mg/ml, 0.015 mg/ml and 0.56 mg/ml respectively.Conclusion: In the present study we achieved enhanced production of psoralen through abiotic elicitors (MeJ and SA) treatment and concluded that the MeJ at 30 µM/l concentration shows significant enhancement of psoralen production.Â

Mitigation of Attacks via Improved Network Security in IoT Network using Machine Learning

In this paper, we develop a support vector machine (SVM) based attack mitigation technique from the IoT network. The SVM aims to classify the features related to the attacks based on pre-processed and feature extracted information. The simulation is conducted in terms of accuracy, precision, recall and f-measure over KDD datasets. The results show that the proposed SVM classifier obtains high grade of classification accuracy in both training and testing datasets

Susceptibility baselines for the invasive mealybugs Phenacoccus manihoti and Paracoccus marginatus (Hemiptera: Pseudococcidae) in cassava ecosystem against selected neonicotinoid insecticides

In recent years, an invasive cassava mealybug Phenacoccus manihoti has been threatening cassava cultivation alongside another invasive papaya mealybug Paracoccus marginatus which invaded the country more than a decade ago. In order to evaluate their responses against the commonly used neonicotinoid insecticides: thiamethoxam 25 WG and imidacloprid 17.8 SL,  acute toxicity experiments to determine the susceptibility baselines in populations of two invasive mealybugs in the cassava agro-ecosystem, namely, cassava mealybug P. manihoti and papaya mealybug P. marginatus were performed upto 15 generations. A systemic uptake method was used for the bioassay. The LC50 values of thiamethoxam for F1 generation were 3.298 ppm whereas it was 1.066 ppm for F15 in cassava mealybug. The LC50 values of F1 generation were 2.014 ppm and that of F15 generation was 1.384 ppm when tested with imidacloprid. In the case of papaya mealybug, the LC50 values ranged from 6.138 ppm (F1) to 2.503 ppm (F15) for thiamethoxam and 7.457 ppm (F1) to 3.231 ppm (F15) for imidacloprid. All the susceptibility indices calculated were less than threefold. The rate of resistance development was negative in all cases showing that none of the tested populations harboured any resistance without insecticidal selection pressure. Tentative discriminating doses were fixed for both chemicals with the help of LC95 values obtained from the bioassay experiments, namely five ppm for both thiamethoxam and imidacloprid in the case of cassava mealybug and 10 ppm and 15 ppm, respectively, for thiamethoxam and imidacloprid in the case of papaya mealybug.          

Cloud Service Selection System Approach based on QoS Model: A Systematic Review

The Internet of Things (IoT) has received a lot of interest from researchers recently. IoT is seen as a component of the Internet of Things, which will include billions of intelligent, talkative "things" in the coming decades. IoT is a diverse, multi-layer, wide-area network composed of a number of network links. The detection of services and on-demand supply are difficult in such networks, which are comprised of a variety of resource-limited devices. The growth of service computing-related fields will be aided by the development of new IoT services. Therefore, Cloud service composition provides significant services by integrating the single services. Because of the fast spread of cloud services and their different Quality of Service (QoS), identifying necessary tasks and putting together a service model that includes specific performance assurances has become a major technological problem that has caused widespread concern. Various strategies are used in the composition of services i.e., Clustering, Fuzzy, Deep Learning, Particle Swarm Optimization, Cuckoo Search Algorithm and so on. Researchers have made significant efforts in this field, and computational intelligence approaches are thought to be useful in tackling such challenges. Even though, no systematic research on this topic has been done with specific attention to computational intelligence. Therefore, this publication provides a thorough overview of QoS-aware web service composition, with QoS models and approaches to finding future aspects