Effects of local simvastatin on periosteal distractiono steogenesis in rabbits

PubMedID: 25640700Our aim was to evaluate the effect of local simvastatin on the formation of new bone using a new design of periosteal distractor. The distractorswere placed between the periosteum and bone at the inferior border of the mandible of 20 New Zealand rabbits. In the first group (n = 10)simvastatin was applied locally to the distraction zone. The other 10 rabbits served as controls. The formation of new bone was evaluated withdigital direct radiography, computed tomography (CT), and histomorphometric analyses. New bone formed in all rabbits, but more formedin the experimental group according to histomorphometric variables. However, other measurements did not differ significantly between thegroups. The new design of the periosteal distraction device was successful in causing new bone to form. Local simvastatin made no significantcontribution to the procedure. © 2015 The British Association of Oral and Maxillofacial Surgeons.Firat University Scientific Research Projects Management Unit: DHF2012D08The study was approved by the Ethics Review Committee of Cukurova University, conducted in accordance with the guidelines of the Cukurova University Animal Research Centre, and supported by Cukurova University Scientific Research Projects Grant, Adana, Turkey ( DHF2012D08 )

Relationships between microRNA-20a and microRNA-125b expression and apoptosis and inflammation in experimental spinal cord injury

PubMedID: 31397222Objectives: The aim of the study was to determine the relationships between microRNA-20a and microRNA-125b expression and apoptosis and inflammation in a rat model of spinal cord injury (SCI) using microscopy, immunohistochemistry, and molecular biology. Methods: Sixty-one rats were divided into three groups: a control group that was not subjected to any operation; a sham-operated group; and an experimental group that was subjected to spinal cord compression. The experimental group was further subdivided into two subgroups: the experimental control group, which did not receive any drug treatment; and the methylprednisolone treatment group, which received 30 mg/kg methylprednisolone on day 0 followed by 10 mg/kg/day methylprednisolone from days 1–14. Results: Tumor necrosis factor-alpha (TNF-?) and interleukin (IL)-6 levels increased in the experimental control group on days 1 and 3, and decreased in the experimental control group and methylprednisolone treatment group on days 7 and 14. Caspase-3 levels increased in the experimental control group on day 1, and decreased in the experimental control group and methylprednisolone treatment group on days 3, 7, and 14. MicroRNA-20a expression was upregulated in the experimental control group on days 1 and 3, and microRNA-125b expression was downregulated on days 3 and 7. Conclusions: After SCI, upregulated microRNA-20a expression and increased proinflammatory cytokines may lead to an increase in inflammation. MicroRNA-125b may be associated with caspase-3, and microRNA-125b downregulation may inhibit apoptosis. Although the results of this study suggest potential relationships between microRNA-20a and microRNA-125b expression and apoptosis and inflammation in SCI, further studies are needed to confirm microRNA-20a and microRNA-125b as biomarkers in SCI and to develop new strategies for the treatment of SCI. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group

Effects of local simvastatin on periosteal distraction osteogenesis in rabbits

7th ACBID International Oral and Maxillofacial Surgery Congress -- 2013 -- Antalya, TURKEYWOS: 000352083800002PubMed ID: 25640700Our aim was to evaluate the effect of local simvastatin on the formation of new bone using a new design of periosteal distractor. The distractors were placed between the periosteum and bone at the inferior border of the mandible of 20 New Zealand rabbits. In the first group (n = 10) simvastatin was applied locally to the distraction zone. The other 10 rabbits served as controls. The formation of new bone was evaluated with digital direct radiography, computed tomography (CT), and histomorphometric analyses. New bone formed in all rabbits, but more formed in the experimental group according to histomorphometric variables. However, other measurements did not differ significantly between the groups. The new design of the periosteal distraction device was successful in causing new bone to form. Local simvastatin made no significant contribution to the procedure. (C) 2015 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.ACBI

FAST VIDEO SEGMENTATION USING ENCODING COST DATA

This paper presents a simple and effective pre-processing method developed for the segmentation of MPEG compressed video sequences. The proposed method for scene-cut detection only involves computing the number of bits spent for each frame (encoding cost data), thus avoiding decoding the bitstream. The information is separated into I-, P-, B-frames, thus forming 3 vectors which are independently processed by a new peak detection algorithm based on overcomplete lter banks and on joint thresholding using a confidence number. Each processed vector yields a set of candidate frame numbers, i.e. "hints" of positions where scene-cuts may have occurred. The "hints" for all frame types are recombined into one frame sequence and clustered into scene cuts. The algorithm was not designed to distintuish among types of cuts but rather to indicate its position and duration. Experimental results show that the proposed algorithm is effective indetecting abrupt scene changes as well as gradual transitions. For precision demanding applications, the algorithm can be used with alow confidence factor just to select the frames that are worth being investigated by a more complex algorithm. The algorithm is not particularly tailored to MPEG and can be applied to most video compression techniques

The effects of N-methyl-d-aspartate receptor blockade during the early neurodevelopmental period on emotional behaviors and cognitive functions of adolescent wistar rats

PubMedID: 23462878The N-Methyl-d-Aspartate (NMDA) receptor is expressed abundantly in the brain and plays an important role in neuronal development, learning and memory, neurodegenerative diseases, and neurogenesis. In this study, we evaluated the effects of NMDA receptor blockade during the early neurodevelopmental period on exploratory locomotion, anxiety-like behaviors and cognitive functions of adolescent Wistar rats. NMDA receptor hypofunction was induced 7-10 days after birth using MK-801 in rats (0.25 mg/kg twice a day for 4 days via intraperitoneal injection). The open-field (OF), elevated plus maze (EPM) and passive avoidance (PA) tests were used to evaluate exploratory locomotion, anxiety-like behaviors and cognitive functions. In the OF test, MK-801 caused an increase in locomotion behavior (p 0.05). Chronic NMDA receptor blockade during the critical period of maturation for the glutamatergic brain system (postnatal days 7-10) produces locomotor hyperactivity and decreased anxiety levels, but has no significant main effect on cognitive function during adolescence. © 2013 Springer Science+Business Media New York.SYOBAP 2010/0002Acknowledgments This research was supported by Adiyaman University Research Fund (SYOBAP 2010/0002)

The effect of vitamin E and L-carnitine against methotrexate-induced injury in rat testis

PubMedID: 26281314Background/aim: Methotrexate (MTX), used commonly as an antimetabolite drug in cancer therapy, leads to acute toxic side effects in tissues or organs containing rapidly dividing cells, such as bone marrow, gastrointestinal mucosa, and seminiferous tubules. In this study, we investigated the protective effects of vitamin E and L-carnitine against MTX-induced injury in rat testis. Materials and methods: Rats were divided into 4 groups, including the control group. The study took 17 days and the animals received daily doses of 0.5 mL/kg saline, 250 mg/kg vitamin E, or 500 mg/kg L-carnitine intraperitoneally. The experimental groups received 20 mg/kg methotrexate intraperitoneally on days 3 and 10. Results: Weight loss, testicular weight loss and marked histological injuries, increased malondialdehyde levels, and decreased superoxide dismutase levels were only observed in the MTX-treated groups. Vitamin E and L-carnitine treatments did not affect MTX-induced weight loss or testicular weight loss, but they inhibited MTX-induced testicular histological injuries. Vitamin E and L-carnitine treatments suppressed the increases in malondialdehyde levels and the decreases in superoxide dismutase levels. Conclusion: Vitamin E and L-carnitine treatments decreased MTX-induced testicular histological injuries, and these results were supported by biochemical measurements. © TÜBİTAK

Effects of sodium selenite and amiloride on calvarial calcification in closing small cranial defects: Laboratory investigation

PubMedID: 20672896Object. Fresh autogenous bone graft is the most preferred osteoplastic material, whether the purpose is cosmetic, psychological, or for the protection of the brain. These grafts are not rejected and do not react immunologically. The aim of this study was to evaluate the efficacy of autogenous fat rolled with bone dust derived from the bur hole in closing small cranial defects. Additionally, the authors examined the morphological and biochemical effects of Na selenite and amiloride on calvarial calcification. Methods. The study group consisted of 20 domestic pigs. These animals were randomly divided into 4 groups. A bur hole with a diameter of 10 mm was made at the right parietal region in all animals, and then the periosteum around the bur hole was cauterized following exposure of the dura mater. The dura was coagulated with bipolar cautery. Group 1 (controls): only a bur hole was opened, and it was then closed with a mixture of the bone dust that had been created during the opening of the bur hole and fat tissue that was taken from the animal's neck. Group 2 (amiloride): 1 nmol/g body weight of amiloride was applied subcutaneously within 15 minutes after closure of the bur hole with bone dust and fat, and then amiloride was applied once a day for 4 weeks. Group 3 (Na selenite): 30 nmol/g body weight of Na selenite was applied subcutaneously within 30 minutes after closure of the bur hole with bone dust and fat, and then Na selenite was applied once a day for 4 weeks. Group 4 (amiloride and Na selenite): 1 nmol/g body weight of amiloride was applied subcutaneously at 15 minutes, and 30 nmol/g body weight of Na selenite was applied subcutaneously at 30 minutes after closure of the bur hole with bone dust and fat, and these 2 injections were repeated once a day for 4 weeks. At the end of 4 weeks, the animals were anesthetized to evaluate the closure of the bur hole. Tissue samples were obtained for ultrastructural and biochemical examination. Results. The defect was covered with diffuse connective tissue in the control group. Although multiple capillary vessels were present, the authors did not observe osteogenic differentiation. Histological examination of the second group revealed osteogenic changes. Although new matrix was formed, calcification was not detected. The authors observed fibroblast, collagen fibers, and dense connective tissue filled with capillary in the third group of pigs, which had undergone Na selenite application. Calcification was not detected in this group. Both connective and osteogenic tissue were observed in specimens obtained in the fourth group, which had undergone amiloride and Na selenite application. Conclusions. The authors experimentally evaluated the supplementary osteogenic effects of Na selenite and amiloride by using them separately and together. The findings seem promising as a lead-in to new studies in restoring cranial defects

Effects of agmatine sulphate on facial nerve injuries

PubMedID: 28122652Objective: To evaluate the effect of agmatine sulphate on facial nerve regeneration after facial nerve injury using electron and light microscopy. Methods: The study was performed on 30 male Wistar albino rats split into: a control group, a sham-treated group, a study control group, an anastomosis group, and an anastomosis plus agmatine sulphate treatment group. The mandibular branch of the facial nerve was dissected, and a piece was removed for histological and electron microscopic examination. Results: Regeneration was better in the anastomosis group than in the study control group. However, the best regeneration findings were seen in the agmatine sulphate treatment group. There was a significant difference between the agmatine group and the others in terms of median axon numbers (p < 0.004) and diameters (p < 0.004). Conclusion: Agmatine sulphate treatment with anastomosis in traumatic facial paralysis may enhance nerve regeneration. Copyright © JLO (1984) Limited 2017

Nonvascularized Cartilage Grafts Versus Vascularized Cartilage Flaps: Comparison of Cartilage Quality 6 Months After Transfer

PubMedID: 29033290Purpose: Subchondral perfusion of osteochondral grafts has been shown to be important in preventing long-term cartilage degeneration. In carpal reconstruction, subchondral perfusion from the graft bed is limited. This study's purpose was to compare the histological characteristics of cartilage in osteochondral grafts supported by synovial imbibition alone to cartilage of vascularized osteochondral flaps that have both synovial and vascular pedicle perfusion. Methods: Two adjacent osteochondral segments were harvested on the medial femoral trochlea in domestic 6- to 8-month-old pigs. Each segment measured approximately 12 mm × 15 mm × 17 mm. One segment was maintained on the descending geniculate artery vascular pedicle. The adjacent segment was separated from the pedicle to serve as a nonvascularized graft. A thin layer of methylmethacrylate cement was used to line the harvest site defect to prevent vascular ingrowth to the subsequently replaced specimens. The pigs were maintained on a high-calorie feed and returned to ambulation and full weight-bearing on the surgical legs. The animals were sacrificed after 6 months and the specimens were reharvested, sectioned, and examined. The cartilage was graded by 2 pathologists blinded to the origin of specimens as vascularized flaps or nonvascularized grafts. Results: All specimens were assigned scores utilizing the International Cartilage Repair Society grading system. Scoring for chondrocyte viability, cartilage surface morphology, and cell and matrix appearance was significantly higher in the vascularized osteochondral group than in the graft group. Conclusions: When deprived of subchondral perfusion from underlying bone, osteochondral vascularized flaps in an intrasynovial environment demonstrate superior cartilage quality and survival compared with nonvascularized grafts. Clinical relevance: In locations in which perfusion from surrounding bone may be limited (ie, proximal scaphoid or proximal lunate reconstruction), articular reconstruction using vascularized osteochondral flaps will yield superior cartilage organization and architecture than nonvascularized osteochondral grafts. The clinical and functional relevance of this finding requires further study. © 2018 American Society for Surgery of the Han

Therapeutic Potential of Pterostilbene and Resveratrol on Biomechanic, Biochemical, and Histological Parameters in Streptozotocin-Induced Diabetic Rats

Aims. The aim of this study was to investigate the effects of pterostilbene (PTS) (trans-3,5-dimethoxy-4'-hydroxystilbene) and resveratrol (RSV) (trans-3,5,4' trihydroxystilbene) applied at different doses for the treatment of streptozotocin- (STZ-) induced diabetic rats. Materials and Methods. At the end of the 5-week experimental period, the right gastrocnemius muscles of the rats were examined biomechanically, while the left ones were examined histologically. In addition, blood glucose, serum insulin, and malondialdehyde (MDA) levels were analyzed in blood samples taken from the rats. Results. The skeletal muscle isometric contraction forces, which showed a decrease with diabetes, were observed to increase with antioxidant applications. Blood glucose, serum insulin, and MDA levels in diabetic rats approached normal levels after applying PTS. When the electron microscopic images of the rat skeletal muscle were examined, those in the combination treatment group were observed to show a better enhancement in the skeletal muscle morphological structure compared to the other diabetic and treatment groups. Conclusion. According to the findings, we suggest that these antioxidant treatments might have good therapeutic nutraceutical potential for some muscle diseases that coexist with diabetes. These treatments should be comprehensively investigated in the future. © 2018 Bora Tastekin et al.The research was supported by the C¸ ukurova University Scientific Research Foundation (Project no. TSA-2016-4958). Thanks are due to the Sabinsa Corporation for the supply of antioxidants