An Evaluation Of Jordanian In-service EFL Teacher's Training Programs

Tujuan penyelidikan ini untuk menilai keberkesanan dan pengaruh kursus latihan perkhidmatan-dalaman guru-guru EFL terhadap kebolehan dan prestasi guru-guru EFL di sekolah-sekolah di sekitar Jordan. Skop penyelidikan ini adalah tertumpu kepada guru-guru EFL yang pemah menyertai atau yang sedang menjalani kursus latihan perkhidmatan-dalaman di Jordan. The purpose of conducting this study was to evaluate the effectiveness of EFL teachers’ in-service training courses based on the perceptions of teachers towards these courses and to examine the extent to which these courses influence EFL teachers’ performance

Plasma metabolomics profiling identifies new predictive biomarkers for disease severity in COVID-19 patients

Recently, numerous studies have reported on different predictive models of disease severity in COVID-19 patients. Herein, we propose a highly predictive model of disease severity by integrating routine laboratory findings and plasma metabolites including cytosine as a potential biomarker of COVID-19 disease severity. One model was developed and internally validated on the basis of ROC-AUC values. The predictive accuracy of the model was 0.996 (95% CI: 0.989 to 1.000) with an optimal cut-off risk score of 3 from among 6 biomarkers including five lab findings (D-dimer, ferritin, neutrophil counts, Hp, and sTfR) and one metabolite (cytosine). The model is of high predictive power, needs a small number of variables that can be acquired at minimal cost and effort, and can be applied independent of non-empirical clinical data. The metabolomics profiling data and the modeling work stemming from it, as presented here, could further explain the cause of COVID-19 disease prognosis and patient management.</p

Assessment of elemental chemistry, spatial distribution, and potential risks of road-deposited dusts in Sharjah, United Arab Emirates

Abstract Road dust is a major source of pollution in the environment, carrying different pollutants, including heavy metals and metalloids, from one location to another. This study assesses the concentrations of eight heavy metals and one metalloid (Zn, Pb, Mn, Fe, Cr, Cu, Cd, Ni, and As) in dust samples collected from sixty-eight streets of Sharjah, United Arab Emirates using ICP-OES, as well as investigates their effects on both the environment and humans. Mean concentrations of the elements in μg/g across the sites were 392 ± 46 (Zn), 68.28 ± 11.3 (Pb), 1437 ± 67 (Mn), 39,481 ± 4611 (Fe), 460 ± 31 (Cr), 150 ± 44 (Cu), 1.25 ± 0.65 (Cd), 856 ± 72 (Ni), and 0.97 ± 0.28 (As). The Cdeg and ERI calculated from the study were 54.79 and 573, respectively, suggesting varying pollution levels. The highest contributions were from Ni, Cd, Zn, Cu, Cr, and Pb, especially in areas with heavy traffic. The non-carcinogenic risk assessments were generally low for the three routes of exposure, except HQoral that was slightly higher for children. Similarly, none of the elements exhibited any carcinogenic risk except chromium. Overall, the cancer risk is considered low. In view of the limited studies from UAE in relation to the metal content of road-deposited dusts, the current study serves as novel knowledge, especially in the context of geographical areas with a higher occurrence of sandstorms and the presence of particulate matter. The study also adds to the global understanding of the contribution of street dust to environmental pollution and its implications for human health.Abstract Road dust is a major source of pollution in the environment, carrying different pollutants, including heavy metals and metalloids, from one location to another. This study assesses the concentrations of eight heavy metals and one metalloid (Zn, Pb, Mn, Fe, Cr, Cu, Cd, Ni, and As) in dust samples collected from sixty-eight streets of Sharjah, United Arab Emirates using ICP-OES, as well as investigates their effects on both the environment and humans. Mean concentrations of the elements in μg/g across the sites were 392 ± 46 (Zn), 68.28 ± 11.3 (Pb), 1437 ± 67 (Mn), 39,481 ± 4611 (Fe), 460 ± 31 (Cr), 150 ± 44 (Cu), 1.25 ± 0.65 (Cd), 856 ± 72 (Ni), and 0.97 ± 0.28 (As). The Cdeg and ERI calculated from the study were 54.79 and 573, respectively, suggesting varying pollution levels. The highest contributions were from Ni, Cd, Zn, Cu, Cr, and Pb, especially in areas with heavy traffic. The non-carcinogenic risk assessments were generally low for the three routes of exposure, except HQoral that was slightly higher for children. Similarly, none of the elements exhibited any carcinogenic risk except chromium. Overall, the cancer risk is considered low. In view of the limited studies from UAE in relation to the metal content of road-deposited dusts, the current study serves as novel knowledge, especially in the context of geographical areas with a higher occurrence of sandstorms and the presence of particulate matter. The study also adds to the global understanding of the contribution of street dust to environmental pollution and its implications for human health

Multi-Omics Profiling of Candida albicans Grown on Solid Versus Liquid Media

Funding Information: Special thanks to the University of Sharjah for the financial support. Competitive grant No: 2201110155 MHS; Seed grant No: 2001110138. This research is part of the Human Disease Biomarkers Discovery Research Group study. Publisher Copyright: © 2023 by the authors.Candida albicans is a common pathogenic fungus that presents a challenge to healthcare facilities. It can switch between a yeast cell form that diffuses through the bloodstream to colonize internal organs and a filamentous form that penetrates host mucosa. Understanding the pathogen’s strategies for environmental adaptation and, ultimately, survival, is crucial. As a complementary study, herein, a multi-omics analysis was performed using high-resolution timsTOF MS to compare the proteomes and metabolomes of Wild Type (WT) Candida albicans (strain DK318) grown on agar plates versus liquid media. Proteomic analysis revealed a total of 1793 proteins and 15,013 peptides. Out of the 1403 identified proteins, 313 proteins were significantly differentially abundant with a p-value < 0.05. Of these, 156 and 157 proteins were significantly increased in liquid and solid media, respectively. Metabolomics analysis identified 192 metabolites in total. The majority (42/48) of the significantly altered metabolites (p-value 0.05 FDR, FC 1.5), mainly amino acids, were significantly higher in solid media, while only 2 metabolites were significantly higher in liquid media. The combined multi-omics analysis provides insight into adaptative morphological changes supporting Candida albicans’ life cycle and identifies crucial virulence factors during biofilm formation and bloodstream infection.publishersversionpublishe

N6-Acetyl-L-Lysine and p-Cresol as Key Metabolites in the Pathogenesis of COVID-19 in Obese Patients

Despite the growing number of the vaccinated population, COVID-19, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains a global health burden. Obesity, a metabolic syndrome affecting one-third of the population, has proven to be a major risk factor for COVID-19 severe complications. Several studies have identified metabolic signatures and disrupted metabolic pathways associated with COVID-19, however there are no reports evaluating the role of obesity in the COVID-19 metabolic regulation. In this study we highlight the involvement of obesity metabolically in affecting SARS-CoV-2 infection and the consequent health complications, mainly cardiovascular disease. We measured one hundred and forty-four (144) metabolites using ultra high-performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS) to identify metabolic changes in response to SARS-CoV-2 infection, in lean and obese COVID-19 positive (n=82) and COVID-19 negative (n=24) patients. The identified metabolites are found to be mainly correlating with glucose, energy and steroid metabolisms. Further data analysis indicated twelve (12) significantly yet differentially abundant metabolites associated with viral infection and health complications, in COVID-19 obese patients. Two of the detected metabolites, n6-acetyl-l-lysine and p-cresol, are detected only among the COVID-19 cohort, exhibiting significantly higher levels in COVID-19 obese patients when compared to COVID-19 lean patients. These metabolites have important roles in viral entry and could explain the increased susceptibility of obese patients. On the same note, a set of six metabolites associated with antiviral and anti-inflammatory functions displayed significantly lower abundance in COVID-19 obese patients. In conclusion, this report highlights the plasma metabolome of COVID-19 obese patients as a metabolic feature and signature to help improve clinical outcomes. We propose n6-acetyl-l-lysine and p-cresol as potential metabolic markers which warrant further investigations to better understand their involvement in different metabolic pathways in COVID-19.publishedVersio

Design, synthesis and mechanistic anticancer activity of new acetylated 5-aminosalicylate-thiazolinone hybrid derivatives

The development of hybrid compounds has been widely considered as a promising strategy to circumvent the difficulties that emerge in cancer treatment. The well-established strategy of adding acetyl groups to certain drugs has been demonstrated to enhance their therapeutic efficacy. Based on our previous work, an approach of accommodating two chemical entities into a single structure was implemented to synthesize new acetylated hybrids (HH32 and HH33) from 5-aminosalicylic acid and 4-thiazolinone derivatives. These acetylated hybrids showed potential anticancer activities and distinct metabolomic profile with antiproliferative properties. The in-silico molecular docking predicts a strong binding of HH32 and HH33 to cell cycle regulators, and transcriptomic analysis revealed DNA repair and cell cycle as the main targets of HH33 compounds. These findings were validated using in vitro models. In conclusion, the pleiotropic biological effects of HH32 and HH33 compounds on cancer cells demonstrated a new avenue to develop more potent cancer therapies

Exploring the effect of estrogen on Candida albicans hyphal cell wall glycans and ergosterol synthesis

Increased levels of 17-β estradiol (E2) due to pregnancy in young women or to hormonal replacement therapy in postmenopausal women have long been associated with an increased risk of yeast infections. Nevertheless, the effect underlying the role of E2 in Candida albicans infections is not well understood. To address this issue, functional, transcriptomic, and metabolomic analyses were performed on C. albicans cells subjected to temperature and serum induction in the presence or absence of E2. Increased filament formation was observed in E2 treated cells. Surprisingly, cells treated with a combination of E2 and serum showed decreased filament formation. Furthermore, the transcriptomic analysis revealed that serum and E2 treatment is associated with downregulated expression of genes involved in filamentation, including HWP1, ECE1, IHD1, MEP1, SOD5, and ALS3, in comparison with cells treated with serum or estrogen alone. Moreover, glucose transporter genes HGT20 and GCV2 were downregulated in cells receiving both serum and E2. Functional pathway enrichment analysis of the differentially expressed genes (DEGs) suggested major involvement of E2 signaling in several metabolic pathways and the biosynthesis of secondary metabolites. The metabolomic analysis determined differential secretion of 36 metabolites based on the different treatments’ conditions, including structural carbohydrates and fatty acids important for hyphal cell wall formation such as arabinonic acid, organicsugar acids, oleic acid, octadecanoic acid, 2-keto-D-gluconic acid, palmitic acid, and steriacstearic acid with an intriguing negative correlation between D-turanose and ergosterol under E2 treatment. In conclusion, these findings suggest that E2 signaling impacts the expression of several genes and the secretion of several metabolites that help regulate C. albicans morphogenesis and virulence

Potential of CDC25 phosphatases in cancer research and treatment: key to precision medicine

The global burden of cancer continues to rise, underscoring the urgency of developing more effective and precisely targeted therapies. This comprehensive review explores the confluence of precision medicine and CDC25 phosphatases in the context of cancer research. Precision medicine, alternatively referred to as customized medicine, aims to customize medical interventions by taking into account the genetic, genomic, and epigenetic characteristics of individual patients. The identification of particular genetic and molecular drivers driving cancer helps both diagnostic accuracy and treatment selection. Precision medicine utilizes sophisticated technology such as genome sequencing and bioinformatics to elucidate genetic differences that underlie the proliferation of cancer cells, hence facilitating the development of customized therapeutic interventions. CDC25 phosphatases, which play a crucial role in governing the progression of the cell cycle, have garnered significant attention as potential targets for cancer treatment. The dysregulation of CDC25 is a characteristic feature observed in various types of malignancies, hence classifying them as proto-oncogenes. The proteins in question, which operate as phosphatases, play a role in the activation of Cyclin-dependent kinases (CDKs), so promoting the advancement of the cell cycle. CDC25 inhibitors demonstrate potential as therapeutic drugs for cancer treatment by specifically blocking the activity of CDKs and modulating the cell cycle in malignant cells. In brief, precision medicine presents a potentially fruitful option for augmenting cancer research, diagnosis, and treatment, with an emphasis on individualized care predicated upon patients’ genetic and molecular profiles. The review highlights the significance of CDC25 phosphatases in the advancement of cancer and identifies them as promising candidates for therapeutic intervention. This statement underscores the significance of doing thorough molecular profiling in order to uncover the complex molecular characteristics of cancer cells

Design, synthesis and mechanistic anticancer activity of new acetylated 5-aminosalicylate-thiazolinone hybrid derivatives

The development of hybrid compounds has been widely considered as a promising strategy to circumvent the difficulties that emerge in cancer treatment. The well-established strategy of adding acetyl groups to certain drugs has been demonstrated to enhance their therapeutic efficacy. Based on our previous work, an approach of accommodating two chemical entities into a single structure was implemented to synthesize new acetylated hybrids (HH32 and HH33) from 5-aminosalicylic acid and 4-thiazolinone derivatives. These acetylated hybrids showed potential anticancer activities and distinct metabolomic profile with antiproliferative properties. The in-silico molecular docking predicts a strong binding of HH32 and HH33 to cell cycle regulators, and transcriptomic analysis revealed DNA repair and cell cycle as the main targets of HH33 compounds. These findings were validated using in vitro models. In conclusion, the pleiotropic biological effects of HH32 and HH33 compounds on cancer cells demonstrated a new avenue to develop more potent cancer therapies