In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions

Aim: This study aimed to show the effects of glutathione, recognized by its antioxidant specialties, on the potential DNA damage (8-hydroxy-2- deoxyguanosine) and the antioxidant system changes upon its implementation in BHK-21 cells cultured with high glucose. Materials and Methods: BHK-21 cell line was regularly surpassed in vitro conditions (5% FBS, 10% horse serum, 1% L-Glutamine, 1% penicillin/ streptomycin in RPMI 1640 medium, and 5% CO2 and 95% humidity and 37ºC) incubated. The control group determined glucose's IC50 value based on the viability tests executed on MTT cells. Cells were seeded in plates as each would have 2x106 cells. The control, the test, and the crossbreed test (glucose; (285 mM), glutathione (250 &micro ,M)) groups were prepared. After 24 hours of incubation, trypsinized cells were designed for analysis through vitrification. In the lysate of the cell culture that was procured, Oxidative DNA damage, TAS, TSO, and OSI were measured by the spectrophotometric system with ELISA. Results: It was observed that 8-OHdG levels increased significantly with glucose application. Moreover, the increase in the HG+GSH group was more significant when compared to the control group (p≤0.05). No difference with the control group was found only in the group where GSH was applied. As for TAS, whereas any difference was observed in GSH used groups, the increase in the HG+GSH group was significant compared to the control group (p≤0.05). that were the same as the control group. TOS and OSI considerably increased in HG + GSH implemented groups as to the control group (p≤0.05). Conclusion: According to the results, no protective impacts of glutathione at the cellular level in the doses mentioned above were observed on high-dose glucose implemented cells. On the other hand, it was revealed that the applied amounts of glutathione in the process did not cause any toxic effects

Investigation of the effect of vitamin c and vitamin e on serum protein fractions in rats with experimental fluorosis

Bu çalışma, deneysel olarak kronik florozis oluşturulan ratlarda vitamin C (vit C) ve E (vit E)'nin serum protein fraksiyonları üzerindeki etkilerini araştırmak amacıyla planlandı. Materyal olarak Wistar-Albino ratlar kullanıldı (Her biri 8 rat içeren, 8 grup). Deneme grupları; kontrol grubu, deneysel florozis grubu, koruma grupları (16 hafta boyunca, içme suyu içeren günlük 150 ppm NaF içeren içme suyu/ad-libitum ile birlikte vit C (100 mg / kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) ve tedavi grupları (16 hafta boyunca 150 ppm NaF içeren içme suyu/ad-libitum verildikten sonra tedavi amacıyla 4 hafta vit C (100 mg/kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) olarak oluşturuldu. Deneme sonunda kan örnekleri toplandı ve serumlar alındı. Bu örneklerde serum protein fraksiyonları selüloz-asetat elektroforezi ile belirlendi. Deneysel florozis grubunda total protein yüzde gram açısından, alfa-1’in önemli oranda azaldığı ve beta globülinlerin arttığı tespit edildi. Korunma ve tedavi amacıyla vitamin C, vitamin E ve vitamin C+vitamin E birlikte verildiği gruplarda, değişen bu değerlerin kontrol grubuna yaklaştığı görüldü.The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) on the serum protein fractions in rats with experimentally-induced chronic fluorosis. Wistar-Albino rats (male) were used as material (8 groups, each containing 8 rats). The experimental groups were control, protection groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, and, on alternate Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C+ Vit E (100 mg/kg + 300 mg/kg) for 16 weeks) therapy groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, then normal drinking water as ad-libitum, Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C + Vit E (100 mg/kg + 300 mg/kg) on alternate for 4 weeks). The end of experimental period, blood samples were collected and serums were obtained. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Alpha-1 was significantly decreased and beta globulins increased in the experimental fluorosis group. In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group

Structural, spectroscopic and quantum chemical studies on copper(II) complex of 4-ethoxy-2-methyl-5-(4-morpholiny1)-3(2H)-pyridazinone

Dede, Bulent/0000-0003-1416-7373WOS: 000442193700052The Cu(II) complex compound (Cu(C11H17N3O3)(2)CL2(H2O)(2)) was synthesized from reaction between the copper(II) chloride dihydrate and the 4-ethoxy-2-methyl-5-(4-morpholinyl)-3(2H)-pyridazinone. The Cu(II) complex compound in octahedral geometry was characterized using elementel analysis, FT-IR and UV-Vis. spectroscopic techniques. The molar conductance and magnetic susceptibility of the Cu(II) complex were experimentally investigated to confirm octahedral geometry. The quantum chemical computations for molecular geometric parameters, vibrational wavenumber, UV Vis. parameters, HOMO-LUMO investigations and NBO analysis of the complex compound were performed by using UHSEh1PBE functional in DFT method at the LanL2DZ basis set. The vibrational analysis was performed to determine metal-ligand bond and ligand vibrations. The HOMO and LUMO analyses were investigated to understand charge transfers and electronic transitions in the complex. The electronic configuration, natural charge and coordination environment of the Cu(II) metal ion was investigated via NBO analysis. The experimental results were compared with computed data. (C) 2018 Published by Elsevier B.V

Study on the 4-ethoxy-2-methyl-5-(4-morpholinyl)-3(2H)pyridazinone using FT-IR, H-1 and C-13 NMR, UV-vis spectroscopy, and DFT/HSEH1PBE method

In this work, the 4-ethoxy-2-methyl-5-(4-morpholinyl)-3(2H)-pyridazinone (or emarfazone, C11H17N3O3) compound, which has many biological functions, has been investigated using FT-IR, H-1 and C-13 NMR (in CDCl3 solvent), and UV-vis (in ethanol solvent) spectroscopic techniques. Furthermore, the optimized molecular structure, conformational analysis, vibrational frequencies and their assignments, H-1 and C-13 NMR chemical shift values (in gas phase and CHCl3 solvent), HOMO-LUMO, MEP (molecular electrostatic potential), NBO (natural bond orbital) analyses, and nonlinear optical (NLO) parameters of the title compound in the ground state have been explored by using DFT/HSEH1PBE method with the 6-311++ G(d, p) basis set. The electronic absorption maximum wavelengths and oscillator strengths (in gas phase and ethanol solvent) were also obtained at TD-DFT/HSEH1PBE level. A comparison among the experimental and calculated results at the mentioned level indicates that the vibrational frequencies and maximum electronic absorption wavelengths are in good agreement with each other

Spectroscopic and quantum chemical calculation study on 2-ethoxythiazole molecule

In this study, the 2-ethoxythiazole molecule (C5H7NSO) which is a member of the five-membered heterocyles with one nitrogen atom group has been investigated by using the experimental UV-vis (in three different solvents) and FT-IR spectral results as well as some magnetic properties. Furthermore, the calculated molecular geometric parameters, vibrational wavenumbers, HOMO-LUMO energies, H-1 and C-13 NMR chemical shift values and natural bond orbitals (NBO) of the title molecule have been calculated at the B3LYP and HSEH1PBE levels of theory with the 6-311++G(d,p) basis set. The spectral results obtained from the quantum chemical calculations are in good agreement with the experimental results. (C) 2017 Elsevier B.V. All rights reserved

Florozisli Koyunlarda ACE Aktivitesi

Bu çalışma, florozis tespit edilen koyunlarda proinflamatuvar bir enzim olan ACE (Anjiyotensin Dönüştürücü Enzim) aktivitesinin belirlenmesi amacıyla planlandı. Hayvanların verimlerindeki kayıplar nedeniyle de ekonomik bir sorun oluşturan doğal florozis, endemik olarak Türkiye’de birçok bölgede insan ve hayvan sağlığını tehdit etmektedir. Endemik florozisin gözlendiği Van ili ve çevresinde yetiştirilen florozis tespit edilen 15 koyun ve klinik olarak sağlıklı tespit edilen 10 koyun olmak üzere toplam 25 koyundan alınan kan örneklerinde serum ACE aktivitesi ölçüldü. Florozisli koyunlarda ACE aktivitesi çok az düşük olarak ölçülse de, istatistiki olarak değerlendirildiğinde florozisli koyunlar ile sağlıklı koyunlarda ACE aktivitesinde önemli bir fark tespit edilemediThis study has been planned to detect ACE (Angiotensin-Converting Enzyme) activity which is a proinflammatory enzyme in fluorosis-diagnosed sheep. Natural fluorosis constitutes an economic problem by causing efficiency losses in animals and it threatens human and animal health in several regions of Turkey endemically. Serum ACE activity was seen in blood samples taken from 15 fluorosis-diagnosed sheep bred in Van province and its neighboring areas where endemic fluorosis are seen and with 10 sheep clinically diagnosed as healthy, 25 sheep in total. Although ACE activity was detected low in sheep with fluorosis, no significant difference was found between fluorosis-diagnosed and healthy sheep statistically

ACE activity in sheep with fluorosis

Bu çalışma, florozis tespit edilen koyunlarda proinflamatuvar bir enzim olan ACE (Anjiyotensin Dönüştürücü Enzim) aktivitesinin belirlenmesi amacıyla planlandı. Hayvanların verimlerindeki kayıplar nedeniyle de ekonomik bir sorun oluşturan doğal florozis, endemik olarak Türkiye’de birçok bölgede insan ve hayvan sağlığını tehdit etmektedir. Endemik florozisin gözlendiği Van ili ve çevresinde yetiştirilen florozis tespit edilen 15 koyun ve klinik olarak sağlıklı tespit edilen 10 koyun olmak üzere toplam 25 koyundan alınan kan örneklerinde serum ACE aktivitesi ölçüldü. Florozisli koyunlarda ACE aktivitesi çok az düşük olarak ölçülse de, istatistiki olarak değerlendirildiğinde florozisli koyunlar ile sağlıklı koyunlarda ACE aktivitesinde önemli bir fark tespit edilemedi.This study has been planned to detect ACE (Angiotensin-Converting Enzyme) activity which is a pro- inflammatory enzyme in fluorosis-diagnosed sheep. Natural fluorosis constitutes an economic problem by causing efficiency losses in animals and it threatens human and animal health in several regions of Turkey endemically. Serum ACE activity was seen in blood samples taken from 15 fluorosis-diagnosed sheep bred in Van province and its neighboring areas where endemic fluorosis are seen and with 10 sheep clinically diagnosed as healthy, 25 sheep in total. Although ACE activity was detected low in sheep with fluorosis, no significant difference was found between fluorosis-diagnosed and healthy sheep statistically

Effects of zinc supplementation on DNA damage in rats with experimental kidney deficiency

dede, semiha/0000-0001-5744-6327WOS: 000396260000014PubMed: 27612456This study was carried out to determine the effect of zinc on oxidative DNA damage in rats with experimental acute and chronic kidney deficiency. Six groups of five Wistar-Albino rats each were assigned as controls (C), acute kidney deficiency (AKD), zinc-supplemented (+Zn), acute kidney deficiency, zinc-supplemented (AKD + Zn), chronic kidney deficiency (CKD) and zinc-supplemented chronic kidney deficiency (CKD + Zn). The levels of 8-Oxo-2'-deoxyguanosine (8-OHdG) were determined, being the lowest in the CKD group (p < 0.05), higher in the C group than those of rats with CKD but lower than that of all the other groups (p < 0.05). There were no significant differences between the controls and the CKD + Zn group, or between the AKD and the +Zn groups. Among all groups, the highest 8-OHdG level was found in the AKD + Zn group (p < 0.05). DNA damage was greater in acute renal failure than in rats with chronic renal failure. The DNA damage in the zinc group was significantly higher than in the controls