33 research outputs found
TGF-Ξ²1 expression by glioma C6 cells in vitro
The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor Ξ²1 (TGF-Ξ²1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-Ξ²1 monoclonal antibody. Immunocytochemical staining for TGF-Ξ²1 and p53 was performed. Results: The proportion of TGF-Ξ²1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-Ξ²1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-Ξ²1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-Ξ²1 seems to be responsible for decrease in TGF-Ξ²1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS
Summer temperature increase has distinct effects on the ectomycorrhizal fungal communities of moist tussock and dry tundra in Arctic Alaska
Arctic regions are experiencing the greatest rates of climate warming on the planet and marked changes have already been observed in terrestrial arctic ecosystems. While most studies have focused on the effects of warming on arctic vegetation and nutrient cycling, little is known about how belowground communities, such as fungi rootβassociated, respond to warming. Here, we investigate how longβterm summer warming affects ectomycorrhizal (ECM) fungal communities. We used Ion Torrent sequencing of the rDNA internal transcribed spacer 2 (ITS2) region to compare ECM fungal communities in plots with and without longβterm experimental warming in both dry and moist tussock tundra.Β CortinariusΒ was the most OTUβrich genus in the moist tundra, while the most diverse genus in the dry tundra wasΒ Tomentella. On the diversity level, in the moist tundra we found significant differences in community composition, and a sharp decrease in the richness of ECM fungi due to warming. On the functional level, our results indicate that warming induces shifts in the extramatrical properties of the communities, where the species with mediumβdistance exploration type seem to be favored with potential implications for the mobilization of different nutrient pools in the soil. In the dry tundra, neither community richness nor community composition was significantly altered by warming, similar to what had been observed in ECM host plants. There was, however, a marginally significant increase in OTUs identified as ECM fungi with the mediumβdistance exploration type in the warmed plots. Linking our findings of decreasing richness with previous results of increasing ECM fungal biomass suggests that certain ECM species are favored by warming and may become more abundant, while many other species may go locally extinct due to direct or indirect effects of warming. Such compositional shifts in the community might affect nutrient cycling and soil organic C storage.Plant science
Long-term experimental warming alters community composition of ascomycetes in Alaskan moist and dry arctic tundra
Arctic tundra regions have been responding to global warming with visible changes in plant community composition, including expansion of shrubs and declines in lichens and bryophytes. Even though it is well known that the majority of arctic plants are associated with their symbiotic fungi, how fungal community composition will be different with climate warming remains largely unknown. In this study, we addressed the effects of longβterm (18Β years) experimental warming on the community composition and taxonomic richness of soil ascomycetes in dry and moist tundra types. Using deep Ion Torrent sequencing, we quantified how OTU assemblage and richness of different orders of Ascomycota changed in response to summer warming. Experimental warming significantly altered ascomycete communities with stronger responses observed in the moist tundra compared with dry tundra. The proportion of several lichenized and mossβassociated fungi decreased with warming, while the proportion of several plant and insect pathogens and saprotrophic species was higher in the warming treatment. The observed alterations in both taxonomic and ecological groups of ascomycetes are discussed in relation to previously reported warmingβinduced shifts in arctic plant communities, including decline in lichens and bryophytes and increase in coverage and biomass of shrubs.Plant science
Fungi of the greening Arctic : compositional and functional shifts in response to climatic changes
Β
The rate of climate warming in the Arctic nearly doubles warming in the temperate regions. In the arctic tundra, this warming has already altered vegetation, with strong declines in lichens and mosses and expansion of shrubs. This process called βthe greening of the Arcticβ has important consequences for the global nutrient cycling and emission of green-house gases to the atmosphere. Even though, plant community dynamics has been monitored in tundra, the effect of climate warming on belowground fungi remained largely unknown, despite the known key roles that fungi play providing the plants with water and nutrients in nutrient-poor arctic soils. This thesis addresses the effect of climate warming on arctic soil fungal communities by DNA-metabarcoding. Climate warming had a strong effect on fungal community composition leading to a strong decline in diversity of lichenized, moss-associated, and ectomycorrhizal fungi. Instead, many species of saprotrophic and parasitic fungi took over. These changes are expected to alter nutrient turnover in tundra soils (including decomposition and CO2 flux) and affect populations of plants and animals (for example, caribous that are feeding on lichenized fungi in winter).
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Long-term warming alters richness and composition of taxonomic and functional groups of arctic fungi
Plant science
The Software Package OPTHICK Is Designed for Air Plasma Electrophysical Parameters Calculation in a Microwave Range.
Abstract:
The package is oriented to fast qualitative research in a dialogue mode of the plasma characteristics in a wide interval of parameter values. The package programs permit to solve transfer equation for microwave radiation, to calculate plasma particles concentration, its conductivity, microwave radiation, absorption factors, plasma optical thickness along observation line and plasma body brightness temperature. It enables to diagnose a plasma by a microwave method, to find out and to classify sources of ionizing radiation. The programs are written in C for DOS environment.Note:
Research direction:Programming, parallel computing, multimedi
(n,Ξ±) reactions cross section research at IPPE
An experimental set-up based on an ionization chamber with a Frisch grid and wave form digitizer was used for (n,Ξ±) cross section measurements. Use of digital signal processing allowed us to select a gaseous cell inside the sensitive area of the ionization chamber and determine the target atoms in it with high accuracy. This kind of approach provided us with a powerful method to suppress background arising from the detector structure and parasitic reactions on the working gas components. This method is especially interesting to study neutron reactions with elements for which solid target preparation is difficult (noble gases for example). In the present experiments we used a set of working gases which contained admixtures of nitrogen, oxygen, neon, argon and boron. Fission of 238U was used as neutron flux monitor. The cross section of the (n,Ξ±) reaction for 16O, 14N, 20Ne, 36Ar, 40Ar and the yield ratio Ξ±0/Ξ±1 of 10B(n,Ξ±0) to 10B(n,Ξ±1) reactions was measured for neutron energies between 1.5 and 7 MeV. Additionally a measurement of the 50Cr(n,Ξ±) cross section using a solid chromium target is also reported
TGF-Ξ²1 EXPRESSION BY GLIOMA C6 CELLS IN VITRO
The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor Ξ²1 (TGF-Ξ²1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-Ξ²1 monoclonal antibody. Immunocytochemical staining for TGF-Ξ²1 and p53 was performed. Results: The proportion of TGF-Ξ²1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-Ξ²1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-Ξ²1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-Ξ²1 seems to be responsible for decrease in TGF-Ξ²1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS
ΠΡΠΎΡΠΎΠ½Π½Π°Ρ ΠΌΠ°Π³Π½ΠΈΡΠ½ΠΎ-ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ½Π°Ρ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΡ ΠΊΠ°ΠΊ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Π½ΡΠΉ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΏΡΠΎΡΠ½ΠΎΡΡΠΈ ΠΊΠΎΡΡΠ΅ΠΉ
Aims: 1) To evaluate an association between the fat fraction (FF) and bone mineral density (BMD) measured by localized proton magnetic resonance spectroscopy (1H-MRS) and quantitative computed tomography (QCT) densitometry, respectively, in healthy vertebrae of children after a compression fracture; 2) To compare the FF and BMD values with the severity of the compression vertebrae fractures.Materials and methods: Twenty (20) patients (aged 11.1Β±2.1 years) with a trauma-induced compression vertebral fractures participated in the study. The BMD of L3, L4 vertebrae (mg/cm3) was measured in by QCT (Philips Brilliance 16). FF in the same area was measured from 1H-MR-spectra (STEAM, echo time (TE)=12.8 ms, repetition time (TR)=3000 ms, voxel size=20Γ15Γ10 mm) using Philips Achieva TX 3.0T MRI scanner.Results: Correlation analysis revealed aΒ significant inverse linear correlation (r=-0.55, p=0.0004) between FF and BMD of L3 ΠΈ L4 vertebrae. In addition, in the patients with severe compression vertebral fracture (more than 2 fractured vertebrae) there was aΒ significant increase in FF values and aΒ BMD decrease, compared to the values in the patients with mild fractures (1β2 fractured vertebrae).Conclusion: The correlation suggests that the increase of FF in the bone marrow and the decrease of BMD in children go in parallel. Therefore, 1H-MRS could be an alternative to QCT and dual-energy X-ray absorptiometry. The absence of radiation load allows for recommendation to use 1Π-MRS for screening and follow-up, as well as for the control of BMD.Π¦Π΅Π»ΠΈ: 1)Β ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΠΆΠΈΡΠ° ΠΈΒ ΠΌΠΈΠ½Π΅ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΡΡ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ, ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΠΌΠΈ ΡΒ ΠΏΠΎΠΌΠΎΡΡΡ Π»ΠΎΠΊΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΏΡΠΎΡΠΎΠ½Π½ΠΎΠΉ ΠΌΠ°Π³Π½ΠΈΡΠ½ΠΎ-ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ½ΠΎΠΉ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΠΈ (1Π-ΠΠ Π‘) ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎ-ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄Π΅Π½ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠΈ (ΠΠΠ’Π) ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ, Π² Π½Π΅ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½Π½ΡΡ
ΠΏΠΎΠ·Π²ΠΎΠ½ΠΊΠ°Ρ
ΡΒ Π΄Π΅ΡΠ΅ΠΉ ΠΏΠΎΡΠ»Π΅ ΠΊΠΎΠΌΠΏΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΠ΅Π»ΠΎΠΌΠ°; 2)Β ΡΡΠ°Π²Π½ΠΈΡΡ Π·Π½Π°ΡΠ΅Π½ΠΈΡ ΡΡΠ°ΠΊΡΠΈΠΈ ΠΆΠΈΡΠ° ΠΈΒ ΠΌΠΈΠ½Π΅ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ (ΠΠΠΠ’) ΡΠΎ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ ΡΡΠΆΠ΅ΡΡΠΈ ΠΊΠΎΠΌΠΏΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΠ΅ΡΠ΅Π»ΠΎΠΌΠ° ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΠΈΠΊΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈΒ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΒ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΈΠ½ΡΠ»ΠΈ ΡΡΠ°ΡΡΠΈΠ΅ 20Β ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² (ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ 11,1Β±2,1Β Π³ΠΎΠ΄Π°) ΡΒ ΡΡΠ°Π²ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΊΠΎΠΌΠΏΡΠ΅ΡΡΠΈΠΎΠ½Π½ΡΠΌ ΠΏΠ΅ΡΠ΅Π»ΠΎΠΌΠΎΠΌ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΊΠ°. ΠΠΠΠ’ (ΠΌΠ³/ΡΠΌ3 ) ΠΈΠ·ΠΌΠ΅ΡΡΠ»Π°ΡΡ Π² ΠΏΠΎΠ·Π²ΠΎΠ½ΠΊΠ°Ρ
L3, L4 ΠΏΠΎ Π΄Π°Π½Π½ΡΠΌ ΠΠΠ’Π Π½Π° Π°ΠΏΠΏΠ°ΡΠ°ΡΠ΅ Philips BrillianceΒ 16. ΠΠ½Π°ΡΠ΅Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΆΠΈΡΠ° (Π‘Π) Π² ΡΠΎΠΉ ΠΆΠ΅ ΠΎΠ±Π»Π°ΡΡΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΏΠΎ 1H-ΠΠ -ΡΠΏΠ΅ΠΊΡΡΠ°ΠΌ (ΠΈΠΌΠΏΡΠ»ΡΡΠ½Π°Ρ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ STEAM: Π²ΡΠ΅ΠΌΡ ΡΡ
ΠΎ=12,8 ΠΌΡ, Π²ΡΠ΅ΠΌΡ ΠΏΠΎΠ²ΡΠΎΡΠ΅Π½ΠΈΡ=3000 ΠΌΡ, ΡΠ°Π·ΠΌΠ΅Ρ Π²ΠΎΠΊΡΠ΅Π»Ρ=20Γ15Γ10Β ΠΌΠΌ) ΡΒ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΠ -ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠ° Philips Achieva TX.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎΡΡΠ΅Π»ΡΡΠΈΠΎΠ½Π½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π²ΡΡΠ²ΠΈΠ» ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ ΠΎΠ±ΡΠ°ΡΠ½ΡΡ Π»ΠΈΠ½Π΅ΠΉΠ½ΡΡ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΡ (r=-0,55, p=0,0004) ΠΌΠ΅ΠΆΠ΄Ρ Π·Π½Π°ΡΠ΅Π½ΠΈΡΠΌΠΈ Π‘Π ΠΈΒ ΠΠΠΠ’, ΡΠ°ΡΡΡΠΈΡΠ°Π½Π½ΡΠΌΠΈ Π²Β ΠΏΠΎΠ·Π²ΠΎΠ½ΠΊΠ°Ρ
L3 ΠΈ L4. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΊΠΎΠΌΠΏΡΠ΅ΡΡΠΈΠΎΠ½Π½ΡΠΌ ΠΏΠ΅ΡΠ΅Π»ΠΎΠΌΠΎΠΌ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΊΠ° ΡΡΠΆΠ΅Π»ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ (Π±ΠΎΠ»Π΅Π΅ 2Β ΠΏΠ΅ΡΠ΅Π»ΠΎΠΌΠΎΠ²) Π·Π°ΡΠΈΠΊΡΠΈΡΠΎΠ²Π°Π½ΠΎ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ Π‘Π Π½Π°ΡΡΠ΄Ρ ΡΒ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΠΠΠ’ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΒ Π°Π½Π°Π»ΠΎΠ³ΠΈΡΠ½ΡΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΠ΅ΡΠ΅Π»ΠΎΠΌΠΎΠΌ Π»Π΅Π³ΠΊΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ (1β2 ΠΏΠ΅ΡΠ΅Π»ΠΎΠΌΠ° ΠΏΠΎΠ·Π²ΠΎΠ½ΠΊΠΎΠ²).ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΡΠ²Π»Π΅Π½Π½Π°Ρ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΎΠ½Π½Π°Ρ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΡ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠ΅Π΄ΠΏΠΎΠ»ΠΎΠΆΠΈΡΡ, ΡΡΠΎ Ρ Π΄Π΅ΡΠ΅ΠΉ ΠΏΡΠΎΡΠ΅ΡΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ Π‘Π Π²Β ΠΊΠΎΡΡΠ½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ ΠΈΒ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΠΠΠ’ ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡ ΠΏΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, 1H-MΠ C ΠΌΠΎΠΆΠ½ΠΎ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ Π²Β ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠ²Ρ ΠΠΠ’Π ΠΈΒ Π΄Π²ΡΡ
ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ²ΡΠΊΠΎΠΉ Π°Π±ΡΠΎΡΠ±ΡΠΈΠΎΠΌΠ΅ΡΡΠΈΠΈ. ΠΡΡΡΡΡΡΠ²ΠΈΠ΅ Π»ΡΡΠ΅Π²ΠΎΠΉ Π½Π°Π³ΡΡΠ·ΠΊΠΈ Π½Π° ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄ΠΎΠ²Π°ΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ 1Π-ΠΠ Π‘ Π΄Π»Ρ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ΠΎΠ²ΠΎΠ³ΠΎ ΠΈ Π΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π»Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ ΠΠΠΠ’