The effect of adjunctive chlorhexidine mouthrinse on GCF MMP-8 and TIMP-1 levels in gingivitis : a randomized placebo-controlled study

Peer reviewe

Evaluating the Periodontal Status and Oral Hygiene Habits Among Dental Patients

WOS: 000390100700004Objective: The periodontal status is important for treatment planning. The aim of this cross-sectional study was to determine the periodontal status and to explore the relationship between demographic and socio-economic data and smoking and systemic health and oral hygiene habits of subjects who attended Ege University School of Dentistry. Methods: All subjects were interviewed with a structured questionnaire. Gender, educational status, income profit, smoking habits, systemic problems, dental treatment frequency, and oral hygiene habits were recorded using this questionnaire. The periodontal status was evaluated by CPITN and plaque and gingival indexes. Data were analyzed using a statistical program. Results: When periodontal conditions were investigated, 69.5% of the subjects had gingivitis and 30.5% had periodontitis. The tooth brushing habits and frequency of replacing a new toothbrush were significantly different between the gingivitis and periodontitis groups (p< 0.05). CPI values negatively correlated with financial income and education level (p< 0.05). A positive correlation was observed between the level of education and number of existed teeth (p< 0.001). Conclusion: According to our results, subjects showed poor periodontal health and a lack of oral hygiene habits. Our results showed an extremely high demand for periodontal health service programs. The present study indicates a need for oral health promotion including health education along with oral healthcare service provision

Diş Hekimliği Fakültesine Başvuran Hastalarda Periodontal Durum ve Ağız Bakımı Alışkanlıklarının Değerlendirilmesi

Objective: The periodontal status is important for treatment planning. The aim of this cross-sectional study was to determine the periodontal status and to explore the relationship between demographic and socio-economic data and smoking and systemic health and oral hygiene habits of subjects who attended Ege University School of Dentistry. Methods: All subjects were interviewed with a structured questionnaire. Gender, educational status, income profit, smoking habits, systemic problems, dental treatment frequency, and oral hygiene habits were recorded using this questionnaire. The periodontal status was evaluated by CPITN and plaque and gingival indexes. Data were analyzed using a statistical program. Results: When periodontal conditions were investigated, 69.5% of the subjects had gingivitis and 30.5% had periodontitis. The tooth brushing habits and frequency of replacing a new toothbrush were significantly different between the gingivitis and periodontitis groups (p;lt;0.05). CPI values negatively correlated with financial income and education level (p;lt;0.05). A positive correlation was observed between the level of education and number of existed teeth (p;lt;0.001). Conclusion: According to our results, subjects showed poor periodontal health and a lack of oral hygiene habits. Our results showed an extremely high demand for periodontal health service programs. The present study indicates a need for oral health promotion including health education along with oral healthcare service provision.Amaç: Periodontal durumun bilinmesi, gerekli tedavi girişimlerinin planlanması için önemlidir. Bu kesitsel araştırmada, Ege Üniversitesi Diş Hekimliği Fakültesi'ne başvuran hastaların klinik periodontal durumlarının belirlenmesi ve demografik verileri (yaş, cinsiyet, eğitim seviyesi), sistemik hastalık varlığı, sigara kullanımı ile birlikte ağız bakımı alışkanlıkları arasındaki ilişkiyi incelemek amaçlanmıştır. Yöntemler: Çalışmaya katılan hastalara demografik özellikleri ve ağız bakımı alışkanlıkları ile ilgili bilgi toplamak amacıyla, soru-cevap şeklinde yapılandırılmış anket uygulandı. Anket sorularıyla; çalışmaya katılan bireylerin maddi geliri, eğitim durumu, sigara kullanımı, sistemik problemleri, dental muayene sıklığı ve ağız bakımı alışkanlıklarını içeren veriler elde edildi. Araştırmaya alınan tüm hastaların CPITN, plak indeksi ve gingival indeksi değerleri ölçüldü. Toplanan bilgiler istatistiksel olarak analiz edildi. Bulgular: Periodontal durumlarına göre hastaların %69,5'inde gingivitis, %30,5'inde periodontitis saptandı. Gingivitis ve periodontitis gruplarındaki bireylerin diş fırçalama alışkanlıkları ve diş fırçası de- ğiştirme sıklıkları arasında anlamlı farklılık bulundu (p0,05). Bireylerin maddi gelir ve eğitim seviyesi ile CPI değerleri arasında negatif korelasyon gözlendi (p0,05). Eğitim seviyesi mevcut diş sayısı arasında ise pozitif korelasyon bulundu (p0,001). Sonuç: Anket sorularına verilen yanıtlar doğrultusunda bireylerin etkin ağız bakımı yapmadıkları anlaşılmıştır. Çalışmamızın bulgularına göre diş hekimliği fakültesine tedavi amacıyla başvuran hastaların periodontal tedaviye ve ağız bakımı eğitimine gereksinimi olduğu belirlenmiştir

Effect of platelet-rich plasma on gingival and periodontal ligament fibroblasts: new in-vitro growth assay

The use of platelet-rich plasma (PRP) as a source of growth factors is reported to be beneficial for periodontal regeneration. The aim of this study was to evaluate its effect on gingival and periodontal ligament fibroblast healing on a special growth assay designed by the working group. A wound with a 5 mm of diameter has been performed on periodontal ligament (PDL) and gingival fibroblast (GF) cell cultures. The cell wells were divided into five groups. The control group received only Dulbecco&apos;s modified Eagle&apos;s medium/ Ham&apos;s (DMEM) and the test groups received 0.5% PRP with 1/3 or 1/2 thrombin; 0.1% PRP with 1/3 or 1/2 thrombin. All of the groups were stained with haemotoxylene-eosine on days 2, 5, 7, 9 and 11. Digital screenings were performed on each time stop and the results were interpreted by means of % surface area covered by the cells. The results showed that 0.1% PRP with 1/3 thrombin group have closed up the wound circle in GF group at day 9 and in PDL group at days 9 and 11 with a significant difference when compared with other groups. GF response was significantly better than PDL cell response starting from day 5. Concluding, PRP favored wound closure in PDL and GF cell cultures and the developed growth assay may be utilized in future investigations of the biological basis of periodontal wound healing

Periodontal disease and systemic diseases in an older population

WOS: 000341018300039PubMed ID: 25015876Objective: To evaluate the relationship between older adults' medical and oral conditions and their self-reports of periodontal conditions with clinically obtained data. Background: Concerns about oral health of elders and its association with systemic diseases have been gaining more attention. Methods: A total of 201 older subjects were interviewed about their previous medical and dental histories and were asked to complete a health questionnaire. Each subject received full mouth exam, including counting number of natural teeth remaining, gingival (GI) and plaque index (PI), CPITN and denture status. Results: Elders who completed health questionnaires had mean age of 62.5. Mean CPITN score was 1.62(+/- 1.12), PI was 1.57(+/- 1.48), and GI was 1.55(+/- 1.31). Women had higher prevalence of CVD and osteoporosis than men (p = 0.008, p = 0.0001, respectively). Subjects who reported bleeding upon brushing had higher PI and GI scores (p = 0.03, p = 0.05, respectively). Smokers were more likely to describe their periodontal tissues as unhealthy (72.3% vs. 27.7%, p = 0.01), whereas self-reports of healthy vs. unhealthy gums did not differ between non-smokers. Conclusion: These findings suggest that a number of systemic conditions are associated with indicators of periodontal disease, and self-reports of oral conditions are independent of systemic diseases. (C) 2014 Elsevier Ireland Ltd. All rights reserved

Effect of platelet-rich plasma on gingival and periodontal ligament fibroblasts: new in-vitro growth assay

of platelet-rich plasma (PRP) as a source of growth factors is reported to be beneficial for periodontal regeneration. The aim of this study was to evaluate its effect on gingival and periodontal ligament fibroblast healing on a special growth assay designed by the working group. A wound with a 5 mm of diameter has been performed on periodontal ligament (PDL) and gingival fibroblast (GF) cell cultures. The cell wells were divided into five groups. The control group received only Dulbeccos modified Eagles medium/Hams (DMEM) and the test groups received 0.5% PRP with 1/3 or 1/2 thrombin; 0.1% PRP with 1/3 or 1/2 thrombin. All of the groups were stained with haemotoxylene-eosine on days 2, 5, 7, 9 and 11. Digital screenings were performed on each time stop and the results were interpreted by means of % surface area covered by the cells. The results showed that 0.1% PRP with 1/3 thrombin group have closed up the wound circle in GF group at day 9 and in PDL group at days 9 and 11 with a significant difference when compared with other groups. GF response was significantly better than PDL cell response starting from day 5. Concluding, PRP favored wound closure in PDL and GF cell cultures and the developed growth assay may be utilized in future investigations of the biological basis of periodontal wound healing

OTOJEN DİŞ KEMİK GREFTİNİN BİYOLOJİK ÖZELLİKLERİ VE KLİNİK KULLANIMI

Kemik dokunun tamiri ve yenilenmesinde, allogreft, ksenogreft ve sentetik kemik greftlerinin eksikliklerinin üstesinden gelebilecek ve otojen kemik greftlerine benzer kemik rejenerasyon kapasitesine sahip yeni materyaller geliştirmek için çalışmalar devam etmektedir. Otojen diş kemik greftleri, fiziksel ve biyolojik yapısı kemik doku ile benzer olan bir materyaldir. Bu greft, inorganik ve organik olmak üzere iki bileşene sahiptir. Organik yapı içerisinde yer alan kemik morfogenetik proteinleri, kollajen ve kollajen olmayan proteinler ile inorganik yapının oluşumunda bir iskelet görevi görür. İnorganik yapının büyük bir yüzdesini oluşturan hidroksiapatit kristalleri dokuya dayanıklılık kazandırır veorganik yapı ile birlikte yeni kemik doku oluşumunu sağlar. Bu derleme, geleneksel kemik greft materyallerine göre daha iyi bir alternatif olarak hizmet edebileceği düşünülen otojen dişkemik greftleri hakkında bilgi sunmaktadır