The effect of adjunctive chlorhexidine mouthrinse on GCF MMP-8 and TIMP-1 levels in gingivitis : a randomized placebo-controlled study

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Hyaluronik asit yüklü kitosan nanoparçacıkların, bifosfonat uygulanmış gingival fibroblast, epitel ve osteoblast hücrelerine etkisinin in vitro olarak değerlendirilmesi

Diş Hekimliği Fakültesi, Periodontoloji A.B.D. Araştırma ProjesiAraştırma Projesi elektronik ortamda bulunmaktadır.Proje kapsamında hyaluronik asit yüklü kitosan nanoparçacıklarının bifosfonat kullanımına bağlı çene kemiği osteonekrozunun tedavisinde etkinliğinin incelenmesi amacıyla insan gingival fibroblat, insan epitel hücreleri ve insan osteoblast hücreleri üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Bu çalışmada, nitrojen içeren bifosfonat grubu ilaçlardan zoledronik asit, yara iyileştirmede aktif olarak kullanılan ve ortamın nemliliğini sağlayarak iyileşme prosesini hızlandıran hyaluronik asit ve antibakteriyel özelliklere sahip nanotaşıyıcı olarak kitosan kullanılmıştır. Hyaluronik asitin suda hızlı çözünmesini önlemek ve kitosanın hassas yapısını daha stabil hale getirmek amacıyla sentez koşulları optimize edilmiş ve kitosan-hyaluronik asit nanopartiküllerinin zeta potansiyeli +32, 3 mV olarak bulunmuş, %61, 625 oranında da yüklenme verimi elde edilmiştir. Oluşturulan nanotaşıyıcı sistemin ve zoledronik asitin HGF-1, SaOS-2, HaCAT hücreleri üzerindeki in vitro etkisini incelemek amacıyla sitotoksisite, apoptoz, yara modeli çalışmaları gerçekleştirilmiştir.;Zoledronik asit, osteonekroz, hyaluronik asit, kitosan.;Zoledronic acid, osteonecrosis, hyaluronic acid, chitosan

Evaluating the Periodontal Status and Oral Hygiene Habits Among Dental Patients

WOS: 000390100700004Objective: The periodontal status is important for treatment planning. The aim of this cross-sectional study was to determine the periodontal status and to explore the relationship between demographic and socio-economic data and smoking and systemic health and oral hygiene habits of subjects who attended Ege University School of Dentistry. Methods: All subjects were interviewed with a structured questionnaire. Gender, educational status, income profit, smoking habits, systemic problems, dental treatment frequency, and oral hygiene habits were recorded using this questionnaire. The periodontal status was evaluated by CPITN and plaque and gingival indexes. Data were analyzed using a statistical program. Results: When periodontal conditions were investigated, 69.5% of the subjects had gingivitis and 30.5% had periodontitis. The tooth brushing habits and frequency of replacing a new toothbrush were significantly different between the gingivitis and periodontitis groups (p< 0.05). CPI values negatively correlated with financial income and education level (p< 0.05). A positive correlation was observed between the level of education and number of existed teeth (p< 0.001). Conclusion: According to our results, subjects showed poor periodontal health and a lack of oral hygiene habits. Our results showed an extremely high demand for periodontal health service programs. The present study indicates a need for oral health promotion including health education along with oral healthcare service provision

Effect of platelet-rich plasma on gingival and periodontal ligament fibroblasts: new in-vitro growth assay

The use of platelet-rich plasma (PRP) as a source of growth factors is reported to be beneficial for periodontal regeneration. The aim of this study was to evaluate its effect on gingival and periodontal ligament fibroblast healing on a special growth assay designed by the working group. A wound with a 5 mm of diameter has been performed on periodontal ligament (PDL) and gingival fibroblast (GF) cell cultures. The cell wells were divided into five groups. The control group received only Dulbecco&apos;s modified Eagle&apos;s medium/ Ham&apos;s (DMEM) and the test groups received 0.5% PRP with 1/3 or 1/2 thrombin; 0.1% PRP with 1/3 or 1/2 thrombin. All of the groups were stained with haemotoxylene-eosine on days 2, 5, 7, 9 and 11. Digital screenings were performed on each time stop and the results were interpreted by means of % surface area covered by the cells. The results showed that 0.1% PRP with 1/3 thrombin group have closed up the wound circle in GF group at day 9 and in PDL group at days 9 and 11 with a significant difference when compared with other groups. GF response was significantly better than PDL cell response starting from day 5. Concluding, PRP favored wound closure in PDL and GF cell cultures and the developed growth assay may be utilized in future investigations of the biological basis of periodontal wound healing

Effect of platelet-rich plasma on gingival and periodontal ligament fibroblasts: new in-vitro growth assay

The use of platelet-rich plasma (PRP) as a source of growth factors is reported to be beneficial for periodontal regeneration. The aim of this study was to evaluate its effect on gingival and periodontal ligament fibroblast healing on a special growth assay designed by the working group. A wound with a 5 mm of diameter has been performed on periodontal ligament (PDL) and gingival fibroblast (GF) cell cultures. The cell wells were divided into five groups. The control group received only Dulbecco&apos;s modified Eagle&apos;s medium/ Ham&apos;s (DMEM) and the test groups received 0.5% PRP with 1/3 or 1/2 thrombin; 0.1% PRP with 1/3 or 1/2 thrombin. All of the groups were stained with haemotoxylene-eosine on days 2, 5, 7, 9 and 11. Digital screenings were performed on each time stop and the results were interpreted by means of % surface area covered by the cells. The results showed that 0.1% PRP with 1/3 thrombin group have closed up the wound circle in GF group at day 9 and in PDL group at days 9 and 11 with a significant difference when compared with other groups. GF response was significantly better than PDL cell response starting from day 5. Concluding, PRP favored wound closure in PDL and GF cell cultures and the developed growth assay may be utilized in future investigations of the biological basis of periodontal wound healing