The use of platelet-rich plasma (PRP) as a source of growth factors is
reported to be beneficial for periodontal regeneration. The aim of this
study was to evaluate its effect on gingival and periodontal ligament
fibroblast healing on a special growth assay designed by the working
group. A wound with a 5 mm of diameter has been performed on
periodontal ligament (PDL) and gingival fibroblast (GF) cell cultures.
The cell wells were divided into five groups. The control group
received only Dulbecco's modified Eagle's medium/ Ham's (DMEM) and the
test groups received 0.5% PRP with 1/3 or 1/2 thrombin; 0.1% PRP with
1/3 or 1/2 thrombin. All of the groups were stained with
haemotoxylene-eosine on days 2, 5, 7, 9 and 11. Digital screenings were
performed on each time stop and the results were interpreted by means
of % surface area covered by the cells. The results showed that 0.1%
PRP with 1/3 thrombin group have closed up the wound circle in GF group
at day 9 and in PDL group at days 9 and 11 with a significant
difference when compared with other groups. GF response was
significantly better than PDL cell response starting from day 5.
Concluding, PRP favored wound closure in PDL and GF cell cultures and
the developed growth assay may be utilized in future investigations of
the biological basis of periodontal wound healing
