End of the Universe 12/21/12 For My Father

This poem and its accompanying introduction address the 2012 EuroAmerican-settler hysteria over their misreading of the Mayan nation’s 13th Ba’k’tun (cosmic calendar) expiring. At the core of indigenous cultures is the ethic of continuance, life, and wholeness—not devastation

Confluences and Crossbloods on Turtle Island

In the United States of America today, what Eastern Woodlands nations call Turtle Island, who is permitted to identify as Indigenous is politically and emotionally-charged and often hostilely enforced—by Native and non-Native Americans. This lingering quagmire of colonization carries with it the psychospiritual baggage of unresolved, intergenerational trauma that continues to shape every facet of American society ever more strongly, as culture-shapers push harder and harder to keep the colonial experiences of Indigenous peoples out of educational curricula and the American imagination of who we are as a people. The more Native American history is hidden, the easier it is for factions to invent and enforce colonial ways of conceptualizing Indigenous identity. In addition to this, fighting among Indigenous peoples about identity stains weekly headlines of Indian Country Today, the largest international online newspaper about happenings in Native nations and communities. Terms like “full-blood” and “mixed-blood/part Indian” can be found in most articles, as blood-quantum has become the means by which Native people are expected to define themselves. Online websites staffed by Indigenous people touting lists of “real Indians” and “fake Indians” abound on the Internet. The topic of Government-Issued Indians with Red Cards who have Fed-Wreck (federal recognition) is a colonial process that harms Native peoples and communities, and many traditional Native people speak out against it. What some Traditionals argue is that treaty rights and Indigenous sovereignty can be honored without the need to vet individual Native Americans on blood quantum. Primary among these voices are those Indigenous writers who are re-shaping the colonial narrative of what it means to be mixed-blood in their poetry, autobiographical essays, and fiction writing. (excerpt

Intravital Microscopy of the Inguinal Lymph Node

Lymph nodes (LN's), located throughout the body, are an integral component of the immune system. They serve as a site for induction of adaptive immune response and therefore, the development of effector cells. As such, LNs are key to fighting invading pathogens and maintaining health. The choice of LN to study is dictated by accessibility and the desired model; the inguinal lymph node is well situated and easily supports studies of biologically relevant models of skin and genital mucosal infection

Quality Of The Nuisance Diatom Didymosphenia Geminata For Macroinvertebrate Nutrition In A Southeastern U.S. Hypolimnetic Tailwater River

Didymosphenia geminata is a mat-like, colony forming diatom native to areas of western North America and found in high elevation oligotrophic rivers across the Northern and Southern Hemispheres. The alga has drawn concern in recent decades due to its negative impacts on the community makeup of benthic macroinvertebrates in streams where it is considered a nuisance or invasive species. Diatoms can serve as a major food source for aquatic macroinvertebrates, however colonies of D. geminata are often much larger and more abundant than populations of other diatoms. The C:N ratios of aquatic plant tissue can serve as an indicator of both the availability of nutrients to the plant and the capacity for transfer of available nutrients to primary consumers. In September 2016 we conducted a study in the South Fork Holston River tailwater in eastern TN, USA to determine the nutritional quality of D. geminata for benthic macroinvertebrates as it compares to native tailwater flora. Samples of nine aquatic macrophyte and submergent plant taxa including D. geminata were collected from three tailwater sites downstream of the South Holston Reservoir to better understand the impacts of cool, hypolimnetic releases on plant tissue quality. C:N ratios of all samples were measured using a Flash Elemental Analyzer (Thermo Fisher Scientific). Tissue quality of D. geminata did not differ significantly from any other study taxa. C:N ratios of two angiosperm and one bryophyte species decreased significantly with increasing distance from the dam, and the C:N ratios of two of these species were positively correlated with mean annual mean temperatures. The C:N ratio of a filamentous algae increased significantly moving away from the dam and was negatively correlated with annual mean temperature. Our results indicate that D. geminata may serve as a food source of comparable quality to native floral species for tolerant benthic macroinvertebrate taxa (e.g. Chironomidae), and our data suggest that the impacts of hypolimnetic releases on tissue quality of aquatic plant taxa are mitigated within a relatively short distance from the dam

Expanded Food and Nutrition Education Program (EFNEP) Paraprofessional Supervision: Crucial Components for Program Success

The Expanded Food and Nutrition Education Program (EFNEP) national leadership and university program partners initiated a discussion about EFNEP paraprofessional supervision due to concerns raised by state coordinators and a belief that the quality and adequacy of paraprofessional supervision have a considerable impact on program outcomes and effectiveness. An EFNEP Paraprofessional Supervision Committee was formed and tasked with developing a framework of paraprofessional supervisory components necessary for effective local supervision of EFNEP within various university and state contexts. The committee conceptualized EFNEP supervision as consisting of three crucial components: responsibilities; traits and skills; and critical support. Responsibilities outline the day-to-day tasks that EFNEP supervisors are expected to complete. Traits and skills underscore the qualities and behaviors that assist a supervisor with the effective implementation of duties, and are categorized as essential, important, or helpful. Critical support articulates the core functions that are shared by the EFNEP supervisor and those who support the supervisor and the program, both specifically and more generally, including administrators and directors. The committee outlined a call to action to address the needs expressed by those who implement EFNEP on a daily basis. The committee proposes that applying and further developing these crucial components will strengthen EFNEP supervision and enhance the program’s effectiveness.https://lib.dr.iastate.edu/extension_pubs/1252/thumbnail.jp

Mixed Valvular Disease Following Transcatheter Aortic Valve Replacement: Quantification and Systematic Differentiation Using Clinical Measurements and Image-Based Patient‐Specific In Silico Modeling

Background: Mixed valvular disease (MVD), mitral regurgitation (MR) from pre‐existing disease in conjunction with paravalvular leak (PVL) following transcatheter aortic valve replacement (TAVR), is one of the most important stimuli for left ventricle (LV) dysfunction, associated with cardiac mortality. Despite the prevalence of MVD, the quantitative understanding of the interplay between pre‐existing MVD, PVL, LV, and post‐TAVR recovery is meager. Methods and Results: We quantified the effects of MVD on valvular‐ventricular hemodynamics using an image‐based patient‐specific computational framework in 72 MVD patients. Doppler pressure was reduced by TAVR (mean, 77%; N=72; P<0.05), but it was not always accompanied by improvements in LV workload. TAVR had no effect on LV workload in 22 patients, and LV workload post‐TAVR significantly rose in 32 other patients. TAVR reduced LV workload in only 18 patients (25%). PVL significantly alters LV flow and increases shear stress on transcatheter aortic valve leaflets. It interacts with mitral inflow and elevates shear stresses on mitral valve and is one of the main contributors in worsening of MR post‐TAVR. MR worsened in 32 patients post‐TAVR and did not improve in 18 other patients. Conclusions: PVL limits the benefit of TAVR by increasing LV load and worsening of MR and heart failure. Post‐TAVR, most MVD patients (75% of N=72; P<0.05) showed no improvements or even worsening of LV workload, whereas the majority of patients with PVL, but without that pre‐existing MR condition (60% of N=48; P<0.05), showed improvements in LV workload. MR and its exacerbation by PVL may hinder the success of TAVR

Drosophila melanogaster Myosin-18 Represents a Highly Divergent Motor with Actin Tethering Properties

The gene encoding Drosophila myosin-18 is complex and can potentially yield six alternatively spliced mRNAs. One of the major features of this myosin is an N-terminal PDZ domain that is included in some of the predicted alternatively spliced products. To explore the biochemical properties of this protein, we engineered two minimal motor domain (MMD)-like constructs, one that contains the N-terminal PDZ (myosin-18 M-PDZ) domain and one that does not (myosin-18 M-ΔPDZ). These two constructs were expressed in the baculovirus/Sf9 system. The results suggest that Drosophila myosin-18 is highly divergent from most other myosins in the superfamily. Neither of the MMD constructs had an actin-activated MgATPase activity, nor did they even bind ATP. Both myosin-18 M-PDZ and M-ΔPDZ proteins bound to actin with Kd values of 2.61 and 1.04 μm, respectively, but only about 50–75% of the protein bound to actin even at high actin concentrations. Unbound proteins from these actin binding assays reiterated the 60% saturation maximum, suggesting an equilibrium between actin-binding and non-actin-binding conformations of Drosophila myosin-18 in vitro. Neither the binding affinity nor the substoichiometric binding was significantly affected by ATP. Optical trapping of single molecules in three-bead assays showed short lived interactions of the myosin-18 motors with actin filaments. Combined, these data suggest that this highly divergent motor may function as an actin tethering protein

Mixed Valvular Disease Following Transcatheter Aortic Valve Replacement: Quantification and Systematic Differentiation Using Clinical Measurements and Image-Based Patient‐Specific In Silico Modeling

Background: Mixed valvular disease (MVD), mitral regurgitation (MR) from pre‐existing disease in conjunction with paravalvular leak (PVL) following transcatheter aortic valve replacement (TAVR), is one of the most important stimuli for left ventricle (LV) dysfunction, associated with cardiac mortality. Despite the prevalence of MVD, the quantitative understanding of the interplay between pre‐existing MVD, PVL, LV, and post‐TAVR recovery is meager. Methods and Results: We quantified the effects of MVD on valvular‐ventricular hemodynamics using an image‐based patient‐specific computational framework in 72 MVD patients. Doppler pressure was reduced by TAVR (mean, 77%; N=72; P<0.05), but it was not always accompanied by improvements in LV workload. TAVR had no effect on LV workload in 22 patients, and LV workload post‐TAVR significantly rose in 32 other patients. TAVR reduced LV workload in only 18 patients (25%). PVL significantly alters LV flow and increases shear stress on transcatheter aortic valve leaflets. It interacts with mitral inflow and elevates shear stresses on mitral valve and is one of the main contributors in worsening of MR post‐TAVR. MR worsened in 32 patients post‐TAVR and did not improve in 18 other patients. Conclusions: PVL limits the benefit of TAVR by increasing LV load and worsening of MR and heart failure. Post‐TAVR, most MVD patients (75% of N=72; P<0.05) showed no improvements or even worsening of LV workload, whereas the majority of patients with PVL, but without that pre‐existing MR condition (60% of N=48; P<0.05), showed improvements in LV workload. MR and its exacerbation by PVL may hinder the success of TAVR

Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis

Early microcalcification is a feature of coronary plaques with an increased propensity to rupture and to cause acute coronary syndromes. In this ex vivo imaging study of coronary artery specimens, the non-invasive imaging radiotracer, 18F-fluoride, was highly selective for hydroxyapatite deposition in atherosclerotic coronary plaque. Specifically, coronary 18F-fluoride uptake had a high signal to noise ratio compared with surrounding myocardium that makes it feasible to identify coronary mineralisation activity. Areas of 18F-fluoride uptake are associated with osteopontin, an inflammation-associated glycophosphoprotein that mediates tissue mineralisation, and Runt-related transcription factor 2, a nuclear protein involved in osteoblastic differentiation. These results suggest that 18F-fluoride is a non-invasive imaging biomarker of active coronary atherosclerotic mineralisation