Establishment of efficient method for callus culture and shoot regeneration of local Indian garlic (var. Yamuna safed)

The experiment was designed to investigate the regeneration potentiality of a garlic variety (Yamuna Safed (G1)), and also to develop an efficient protocol for regeneration of garlic via callus culture. Higher percentage of callus was initiated from the combination of BAP and 2, 4 D at 2.0 mg/l and 0.25 mg/l respectively.  The hormone 2, 4-D, commonly used in tissue culture in garlic was found to be detrimental when used at the levels described in literatures. Rooting of individual shoots was induced after transfer to medium without growth regulator. The plantlets were established in the soil after acclimatization. ÂÂ

Establishment of efficient method for callus culture and shoot regeneration of local Indian garlic (var. Yamuna safed)

The experiment was designed to investigate the regeneration potentiality of a garlic variety (Yamuna Safed (G1)), and also to develop an efficient protocol for regeneration of garlic via callus culture. Higher percentage of callus was initiated from the combination of BAP and 2, 4 D at 2.0 mg/l and 0.25 mg/l respectively.  The hormone 2, 4-D, commonly used in tissue culture in garlic was found to be detrimental when used at the levels described in literatures. Rooting of individual shoots was induced after transfer to medium without growth regulator. The plantlets were established in the soil after acclimatization. Â

Embriogénesis somática en Allium sativum L. (cv. Yamuna Safed 3): Mejora de la maduración del embrión y la germinación conPGRs y carbohidratos.

Callus induction was obtained from basal part of the garlic clove on Murashige and Skoog (MS) medium on which somatic embryos were formed. In Allium sativum L. cv. Yamuna Safed 3, somatic embryogeny has been categorized into three distinct stages: initiation and proliferation of embryo; maturation; and germination of embryo. The initiation of globular-stage embryo differentiation was observed on various BAP and 2,4-D concentrations but at 1.0 mg/l BAP and 0.25 mg/l 2,4-D maximum proliferation of embryos was noted. Best somatic embryo maturation was achieved on 0.5 mg/l GA3 amended medium; ABA at the same level was less effective. Embryo growth was maximum on 3% maltose while plantlet conversion was highest (65.15%) on 3% fructose added medium. Scanning electron microscopy (SEM) has been presented to demonstrate the apparent morphology and structural details of embryo. Plantlets were successfully raised from in vitro developed embryos