Histologic Evaluation of the Efficacy of rhBMP-2 Compared With Autograft Bone in Sheep Spinal Anterior Interbody Fusion

Study Design. The sheep anterior lumbar spinal fusion model was used to study the efficacy of recombinant human bone morphogenetic protein-2 (rhBMP-2)–collagen composite in comparison with autograft to enhance spinal interbody fusion. Comparisons were drawn from temporal radiographic and end-point biomechanical and histologic data. Objective. To analyze histologically the ability of rhBMP-2 to achieve complete arthrodesis between vertebral bodies. Summary of Background Data. Studies using rhBMP for enhancement of anterior interbody fusion have used numerous endpoints. However, systematic histologic evaluation of the fusion has not been conducted. Methods. Twelve sheep underwent single-level anterior lumbar interbody fusion performed with a cylindrical fenestrated titanium interbody fusion device (INTER FIX, Medtronic Sofamor Danek, Inc., Memphis, TN). The device was filled either with rhBMP-2–collagen (n = 6) or autogenous iliac crest bone graft (n = 6). Radiologic evaluation was carried out at 2-month intervals, and all sheep were killed 6 months after surgery. Nondestructive biomechanical testing for stiffness to flexion, extension, and lateral bending moments, un-decalcified histology, and qualitative and quantitative histologic evaluation were performed. Results. Radiographs revealed a bony bridge anterior to the cage in five of six rhBMP-2-treated animals, whereas it was present only in one of five in the autogenous bone graft group. Segments treated with rhBMP-2 were 20% stiffer in flexion than autograft-treated segments at 6 months. Six of six in the rhBMP-2 group and two of six in the autograft group showed complete fusion. There was a significantly higher rate of bony continuity observed at the fenestrations of the rhBMP-2 group. Three times more number of cage fenestrations in the rhBMP-2 group demonstrated “all-bone” when compared with the autograft group (P \u3c 0.001). Further, the scar tissue in and around the autograft-treated cages was 16-fold more (P \u3c 0.01) than that seen for rhBMP-2-treated cages. Conclusions. The study demonstrates that rhBMP-2 can lead to earlier radiologic fusion and a more consistent increased stiffness of the segments when compared with autograft in sheep anterior lumbar interbody fusion. Furthermore, a three times higher histologic fusion rate is attainable with significantly reduced fibrous tissue around the implant when rhBMP-2 is used

Bony ingrowth potential of 3D-printed porous titanium alloy: a direct comparison of interbody cage materials in an in vivo ovine lumbar fusion model.

Background contextThere is significant variability in the materials commonly used for interbody cages in spine surgery. It is theorized that three-dimensional (3D)-printed interbody cages using porous titanium material can provide more consistent bone ingrowth and biological fixation.PurposeThe purpose of this study was to provide an evidence-based approach to decision-making regarding interbody materials for spinal fusion.Study designA comparative animal study was performed.MethodsA skeletally mature ovine lumbar fusion model was used for this study. Interbody fusions were performed at L2-L3 and L4-L5 in 27 mature sheep using three different interbody cages (ie, polyetheretherketone [PEEK], plasma sprayed porous titanium-coated PEEK [PSP], and 3D-printed porous titanium alloy cage [PTA]). Non-destructive kinematic testing was performed in the three primary directions of motion. The specimens were then analyzed using micro-computed tomography (µ-CT); quantitative measures of the bony fusion were performed. Histomorphometric analyses were also performed in the sagittal plane through the interbody device. Outcome parameters were compared between cage designs and time points.ResultsFlexion-extension range of motion (ROM) was statistically reduced for the PTA group compared with the PEEK cages at 16 weeks (p-value=.02). Only the PTA cages demonstrated a statistically significant decrease in ROM and increase in stiffness across all three loading directions between the 8-week and 16-week sacrifice time points (p-value≤.01). Micro-CT data demonstrated significantly greater total bone volume within the graft window for the PTA cages at both 8 weeks and 16 weeks compared with the PEEK cages (p-value<.01).ConclusionsA direct comparison of interbody implants demonstrates significant and measurable differences in biomechanical, µ-CT, and histologic performance in an ovine model. The 3D-printed porous titanium interbody cage resulted in statistically significant reductions in ROM, increases in the bone ingrowth profile, as well as average construct stiffness compared with PEEK and PSP

Upper-slope jets and gulf stream filaments inshore of the Charleston Bump during winter 2012

A 3-month-long field program conducted in winter 2012 inshore of the seaward deflection of the Gulf Stream at the Charleston Bump observed several 7-21-day periods of strong (> 0.5ms-1) equatorward alongshelf flow over the upper continental slope. In sea surface temperature images, these phenomena resemble and appear linked to warm filaments, features known to be associated with meanders of the Gulf Stream as it traverses the southeast coast of North America. However, the character of these upper-slope features differs from previous descriptions of filaments, hence we describe them as ''upper-slope jets.'' We document the characteristics of the jets, which are approximately 30 km in width, centered on the 200-m isobath, with a maximum temperature variation at depth, and reasonably long-lived. Southwestward flow within the jet extends to 200m and is in approximate thermal wind balance below a surface mixed layer. Maximum transport is estimated to be about 2.0 Sv (1 Sv ≡ 106m3 s-1), driving a net equatorward along-shelf velocity over the deployment period. For this time period, at least, the jets form the equatorward flow of the shoreward flank of the Charleston Gyre. We suggest the features resemble the Pinocchio's Nose Intrusion recently described by Zhang and Gawarkiewicz. Large-amplitude meander crests with sufficiently strong curvature vorticity are a plausible source of initiation of the upper-slope jets

An observational, spatially explicit, stability-based estimate of the wind resource off the shore of North Carolina

As part of ongoing studies of the feasibility of utility-scale wind energy off the shore of North Carolina, winds at 80-m elevation are estimated with a stability-based height-adjustment scheme. Data sources are level-3 daily Advanced Scatterometer (ASCAT) 10-m wind fields as measured by the MetOp-A satellite, North American Regional Reanalysis (NARR) estimates of near-surface atmospheric temperature and humidity, and the National Climatic Data Center's optimally interpolated Advanced Very High Resolution Radiometer (AVHRR-OI) sea surface temperature (SST). A height-adjustment assuming neutral atmospheric stability provides reference conditions. The SST from AVHRR-OI was more accurate than SST from NARRand was used withNARRatmospheric data to represent atmospheric stability in the study region. The 5-yr average of the ASCAT 10-m winds is 6.5-9.0 ms-1 off the shore of North Carolina, with the strongest winds found over the Gulf Stream. Neutral-scheme 80-m windspeeds are 7.5-10.5 ms-1 and follow the same spatial pattern. The stability-based scheme produces an 80-m wind field with significantly different spatial wind patterns, with greater wind speeds than the neutral scheme in coastal regions where stable atmosphere conditions occur and lesser wind speeds than the neutral scheme farther offshore where unstable conditions are prevalent. The largest differences between the schemes occur in winter and spring when and where stable atmospheric conditions are most common. Estimated power inshore from the 100-m isobath with the neutral scheme yields average values of 400-800 W m-2, whereas the stability-based-scheme values are 600-800 W m-2. Capacity factors vary between 30% and 55%, with values in excess of 40% common in coastal areas off North Carolina

Gulf stream marine hydrokinetic energy off cape hatteras, north carolina

Multi-year measurements of current velocity, salinity, and temperature from fixed and vessel-mounted sensors quantify Gulf Stream (GS) marine hydrokinetic energy (MHK) resource variability and inform development off Cape Hatteras, NC. Vessel transects across the GS demonstrate a jet-like velocity structure with speeds exceeding 2.5 m/s at the surface, persistent horizontal shear throughout the jet, and strongest vertical shears within the cyclonic shear zone. Persistent equatorward flow at the base of the GS associated with the Deep Western Boundary Current (DWBC) produces a local maximum in vertical shear where stratification is weak and is postulated to be a site of strong turbulent mixing. Repeated transects at the same location demonstrate that the velocity structure depends upon whether the GS abuts the shelf slope or is offshore. Currents from a fixed acoustic Doppler current profiler (ADCP) deployed on the shoreward side of the GS exceed 1 m/s 64% of the time 40 m below the surface. The 3.75-year time series of currents from the ADCP mooring document large, roughly weekly variations in downstream and cross-stream speed (−0.5 to 2.5 m/s) and shear (± 0.05 s−1) over the entire water column due to passage of GS meanders and frontal eddies. Current reversals from the mean GS direction occur several times a month, and longer period variations in GS offshore position can result in reduced currents for weeks at a time. Unresolved small-scale shear is postulated to contribute significantly to turbulent mixing

Implementing quality control of high-frequency radar estimates and application to gulf stream surface currents

Quality control procedures based on nonvelocity parameters for use with a short-range radar system are applied with slight modification to long-range radar data collected offshore of North Carolina. The radar footprint covers shelf and slope environments and includes a segment of the Gulf Stream (GS). Standard processed and quality controlled (QCD) radar data are compared with 4 months of acoustic Doppler current profiler (ADCP) time series collected at three different sites within the radar footprint. Two of the ADCP records are from the shelf and the third is on the upper slope and is frequently within the GS. Linear regression and Bland-Altman diagrams are used to quantify the comparison. QCD data at all sites have reduced scatter and improved correlation with ADCP observations relative to standard processed data. Uncertainty is reduced by approximately 20%, and linear regression slopes and correlation coefficients increase by about 0.1. At the upper slope site, the QCD data also produced a significant increase in the mean speed. Additionally, a significant increase, averaging roughly 20%, in mean speed in the GS is apparent when comparing standard processed data and QCD data, concentrated at large range and at the azimuthal extremes of radial site coverage. Shifts in the distributions of the standard processed and QCD velocity estimates are consistent with the removal of zero-mean noise from the observations, which has minimal impact where the radial site range is < 70 km and a large impact at greater range in the GS where mean currents exceed 1 m s-1

Effects of intratidal and tidal range variability on circulation and salinity structure in the Cape Fear River Estuary, North Carolina

Tidal influences on circulation and the salinity structure are investigated in the largely unstudied Cape Fear River Estuary (CFRE), North Carolina, a partially mixed estuary along the southeast coast of the United States. During two different tidal conditions (high versus low tidal range) and when river flow was low, salinity and velocity data were collected over a semidiurnal tidal cycle in a 2.8 km long transect along the estuary axis. Water level data were recorded nearby. Mechanisms that influence salt transport characteristics are diagnosed from an analysis of the field data. Specifically, we investigated the relationship between tidal range and salinity through comparison of along-channel circulation characteristics, computed salt fluxes, and coefficients of vertical eddy diffusivity (Kz) based on a parameterization and on salt budget analysis. Findings indicate up-estuary tidally driven salt fluxes resulting from oscillatory salt transport are dominant near the pycnocline, while mean advective transport dominates near the bottom during both tidal range periods. Earlier research related to salt transport in estuaries with significant gravitational circulation suggests that up-estuary salt transport increases during low tidal ranges as a result of increased gravitational circulation. In the CFRE, in contrast, net (tidally averaged) near-bottom along-channel velocities are greater during higher tidal range conditions than during lower tidal range conditions. Findings indicate stronger tidal forcing and associated mixing contribute to greater near-bottom salinity gradients and, consequently, increased baroclinic circulation. Lower near-bottom salinities during the higher tidal range period are a result of a combination of increased vertical turbulent salt fluxes near the pycnocline and increased bottom-generated mixing

Cold-water coral growth under extreme environmental conditions, the Cape Lookout area, NW Atlantic

The Cape Lookout cold-water coral area off thecoast of North Carolina forms the shallowest and northernmostcold-water coral mound area on the Blake Plateau inthe NW Atlantic. Cold-water coral habitats near Cape Lookoutare occasionally bathed in the Gulf Stream, which is characterisedby oligotrophic warm water and strong surface currents.Here, we present the first insights into the mound distributionand morphology, sedimentary environment and coralcover and near-bed environmental conditions as recordedby bottom landers from this coral area. The mounds occurbetween 320 and 550m water depth and are characterisedby high acoustic backscatter indicating the presenceof hard structure. Three distinct mound morphologies wereobserved: (1) a mound with a flattened top at 320 m, (2)multi-summited mounds with a teardrop shape in the middlepart of the area and (3) a single mound at 540m water depth.Echosounder profiles show the presence of a strong reflectorunderneath all mound structures that forms the base of themounds. This reflector cropped out at the downstream side ofthe single mound and consists of carbonate slabs. Video analysisrevealed that all mounds are covered by Lophelia pertusaand that living colonies only occur close to the summitsof the SSW side of the mounds, which is the side that facesthe strongest currents. Off-mound areas were characterisedby low backscatter and sediment ripples, indicating the presenceof relatively strong bottom currents. Two bottom landerswere deployed amidst the coral mounds between December2009 and May 2010. Both landers recorded prominentevents, characterised by large fluctuations in environmentalconditions near the seabed as well as in the overlyingwater column. The period between December and April wascharacterised by several events of increasing temperature andsalinity, coinciding with increased flow and near-bed acousticbackscatter. During these events temperature fluctuatedby up to 9 ?C within a day, which is the largest temperaturevariability as measured so far in a cold-water coral habitat.Warm events, related to Gulf Stream meanders, had the durationof roughly 1 week and the current during these eventswas directed to the NNE. The consequences of such eventsmust be significant given the strong effects of temperature on the metabolism of cold-water corals. Furthermore, elevatedacoustic backscatter values and high mass fluxes werealso recorded during these events, indicating a second stressorthat may affect the corals. The abrasive nature of sand incombination with strong currents might sand blast the corals.We conclude that cold-water corals near Cape Lookout liveunder extreme conditions that limit mound growth at present

Sustained in situ measurements of dissolved oxygen, methane and water transport processes in the benthic boundary layer at MC118, northern Gulf of Mexico

Within months of the BP Macondo Wellhead blowout, elevated methane concentrations within the water column revealed a significant retention of light hydrocarbons in deep waters plus corresponding dissolved oxygen (DO) deficits. However, chemical plume tracking efforts were hindered by a lack of in situ monitoring capabilities. Here, we describe results from in situ time-series, lander-based investigations of physical and biogeochemical processes controlling dissolved oxygen, and methane at Mississippi Canyon lease block 118 (~18 km from the oil spill) conducted shortly after the blowout through April 2012. Multiple sensor arrays plus open-cylinder flux chambers ("chimneys") deployed from a benthic lander collected oxygen, methane, pressure, and current speed and direction data within one meter of the seafloor. The ROVARD lander system was deployed for an initial 21-day test experiment (9/13/2010-10/04/2010) at 882 m depth before a longer 160-day deployment (10/24/2011-4/01/2012) at 884 m depth. Temporal variability in current directions and velocities and water temperatures revealed strong influences of bathymetrically steered currents and overlying along-shelf flows on local and regional water transport processes. DO concentrations and temperature were inversely correlated as a result of water mass mixing processes. Flux chamber measurements during the 160-day deployment revealed total oxygen utilization (TOU) averaging 11.6 mmol/m2 day. Chimney DO concentrations measured during the 21-day deployment exhibited quasi-daily variations apparently resulting from an interaction between near inertial waves and the steep topography of an elevated scarp immediately adjacent to the 21-day deployment site that modulated currents at the top of the chimney. Variability in dissolved methane concentrations suggested significant temporal variability in gas release from nearby hydrocarbon seeps and/or delivery by local water transport processes. Free-vehicle (lander) monitoring over time scales of months to years utilizing in situ sensors can provide an understanding of processes controlling water transport, respiration and the fate and impacts of accidental and natural gas and oil releases

A compact laboratory transmission X-ray microscope for the water window

In the water window (2.2-4.4 nm) the attenuation of radiation in water is significantly smaller than in organic material. Therefore, intact biological specimen (e.g. cells) can be investigated in their natural environment. In order to make this technique accessible to users in a laboratory environment a Full-Field Laboratory Transmission X-ray Microscope (L-TXM) has been developed. The L-TXM is operated with a nitrogen laser plasma source employing an InnoSlab high power laser system for plasma generation. For microscopy the Ly α emission of highly ionized nitrogen at 2.48 nm is used. A laser plasma brightness of 5 × 1011 photons/(s × sr × μm2 in line at 2.48 nm) at a laser power of 70 W is demonstrated. In combination with a state-of-the-art Cr/V multilayer condenser mirror the sample is illuminated with 106 photons/(μm2 × s). Using objective zone plates 35-40 nm lines can be resolved with exposure times < 60 s. The exposure time can be further reduced to 20 s by the use of new multilayer condenser optics and operating the laser at its full power of 130 W. These exposure times enable cryo tomography in a laboratory environment