Creep failure of honeycombs made by rapid prototyping

Additive manufacture and rapid prototyping are versatile methods for the generation of lattice materials for applications in the creep regime. However, these techniques introduce defects that can degrade the macroscopic creep strength. In the present study, the uniaxial tensile response of two-dimensional PMMA lattices is measured in the visco-plastic regime: tests are performed at 100 °C which is slightly below the glass transition temperature Tg of PMMA. Both as-manufactured defects (Plateau borders and strut thickness variation) and as-designed defects (missing cell walls, solid inclusions, and randomly perturbed joints) are introduced. The dispersion in macroscopic strength is measured for relative densities in the range of 0.07–0.19. It is observed that initial failure of the lattice is diffuse in nature: struts fail at a number of uncorrelated locations, followed by the development of a single macroscopic crack transverse to the loading direction. In contrast, the same PMMA lattice fails in a correlated, brittle manner at room temperature. An FE study is performed to gain insight into the diffuse failure mode and the role played by as-manufactured defects, including the dispersion in tensile strength of individual struts of the lattice. A high damage tolerance to as-designed defects is observed experimentally: there is negligible knock-down in strength due to the removal of cell walls or to the presence of solid inclusions. These findings aid the design and manufacture of damage tolerant lattices in the creep regime.The authors gratefully acknowledge the financial support from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program, grant GA669764, MULTILAT

Regulation of notch sensitivity of lattice materials by strut topology

We propose a local reinforcement technique for lattices in the vicinity of a stress-raiser such as a notch, in order to elevate the macroscopic strength and ductility. A spatially non-uniform waviness distribution of sinusoidally-shaped struts is assumed in the vicinity of the notch, and the sensitivity of macroscopic tensile response to strut waviness distribution is studied by finite element analysis. Optimized lattice structures are determined in order to maximise the macroscopic tensile strength or ductility from these various strut waviness distributions. Both hexagonal and triangular lattices are studied as these geometries are representative of bending-dominated and stretching-dominated lattices, respectively

The Influence of Strut Waviness on the Tensile Response of Lattice Materials

Abstract Recent advances in additive manufacturing methods make it possible, for the first time, to manufacture complex micro-architectured solids that achieve desired stress versus strain responses. Here, we report experimental measurements and associated finite element (FE) calculations on the effect of strut shape upon the tensile response of two-dimensional (2D) lattices made from low-carbon steel sheets. Two lattice topologies are considered: (i) a stretching-dominated triangular lattice and (ii) a bending-dominated hexagonal lattice. It is found that strut waviness can enhance the ductility of each lattice, particularly for bending-dominated hexagonal lattices. Manufacturing imperfections such as undercuts have a small effect on the ductility of the lattices but can significantly reduce the ultimate tensile strength. FE simulations provide additional insight into these observations and are used to construct design maps to aid the design of lattices with specified strength and ductility.</jats:p

An assessment of the J-integral test for a metallic foam

An assessment is made of the J-integral test procedure for initial crack growth in an open-cell aluminium alloy foam by combining finite element (FE) simulations with experiment. It is found experimentally that a zone of randomly failed struts develops ahead of the primary crack tip, and is comparable in size to that of the plastic zone. Hence, a crack tip J-field is absent at the initiation of crack growth from the primary crack tip. This implies that the measured J_IC value and the J versus crack extension Da curve cannot be treated as material properties despite the fact that the specimen size meets the usual criteria for J validity. The toughness tests were performed on a single-edge notched bend specimen, and crack extension was measured by the direct current potential drop method, by digital image correlation and by X-ray computed tomography. The crack growth resistance of the foam is associated with two distinct zones of plastic dissipation: (i) a bulk plastic zone emanating from the crack tip (containing a cluster of randomly failed struts), and (ii) a crack bridging zone behind the advancing crack tip. The applicability of a cohesive zone model to predict the fracture response is explored for the observed case of large scale bridging. To do so, FE simulations are performed by replacing the discrete lattice of the open-cell metallic foam by a compressible, elastic-plastic hardening solid while the fracture process zone in the foam is represented by a cohesive zone, as characterised by a tensile traction versus separation law. A detailed comparison of the cohesive zone model with experimental observations reveals that it is possible to capture the load versus displacement response but not the details of the fracture process zone using a single set of process zone parameters

The role of defects in dictating the strength of brittle honeycombs made by rapid prototyping

Rapid prototyping is an emerging technology for the fast make of engineering components. A common technique is to laser cut a two-dimensional (2D) part from polymethyl methacrylate (PMMA) sheet. However, both manufacturing defects and design defects (such as stress raisers) exist in the part, and these degrade its strength. In the present study, a combination of experiment and finite element analysis is used to determine the sensitivity of the tensile strength of PMMA hexagonal lattices to both as-manufactured and as-designed defects. The as-manufactured defects include variations in strut thickness and in Plateau border radius. The knockdown in lattice tensile strength is measured for lattice relative density in the range of 0.07 to 0.19. A systematic finite element (FE) study is performed to assess the explicit role of each type of as-manufactured defect on the lattice strength. As-designed defects such as randomly perturbed joints, missing cells, and solid inclusions are introduced within a regular hexagonal lattice. The notion of a transition flaw size is used to quantify the sensitivity of lattice strength to defect size.The authors gratefully acknowledge the financial support from the European Research Council under the European Union's Horizon 2020 research and innovation program, grant GA669764, MULTILAT

Transplantation of Photoreceptor and Total Neural Retina Preserves Cone Function in P23H Rhodopsin Transgenic Rat

Background: Transplantation as a therapeutic strategy for inherited retinal degeneration has been historically viewed to restore vision as a method by replacing the lost retinal cells and attempting to reconstruct the neural circuitry with stem cells, progenitor cells and mature neural retinal cells. Methods and Findings: We present evidence for an alternative strategy aimed at preventing the secondary loss of cones, the most crucial photoreceptors for vision, by transplanting normal photoreceptors cells into the eye of the P23H rat, a model of dominant retinitis pigmentosa. We carried out transplantation of photoreceptors or total neural retina in 3-monthold P23H rats and evaluated the function and cell counts 6 months after surgery. In both groups, cone loss was significantly reduced (10%) in the transplanted eyes where the cone outer segments were found to be considerably longer. This morphological effect correlated with maintenance of the visual function of cones as scored by photopic ERG recording, but more precisely with an increase in the photopic b-wave amplitudes by 100 % and 78 % for photoreceptor transplantation and whole retinal transplantation respectively. Conclusions: We demonstrate here that the transplanted tissue prevents the loss of cone function, which is furthe

Protocol design and current status of CLIVIT: a randomized controlled multicenter relevance trial comparing clips versus ligatures in thyroid surgery

BACKGROUND: Annually, more than 90000 surgical procedures of the thyroid gland are performed in Germany. Strategies aimed at reducing the duration of the surgical procedure are relevant to patients and the health care system especially in the context of reducing costs. However, new techniques for quick and safe hemostasis have to be tested in clinically relevance randomized controlled trials before a general recommendation can be given. The current standard for occlusion of blood vessels in thyroid surgery is ligatures. Vascular clips may be a safe alternative but have not been investigated in a large RCT. METHODS/DESIGN: CLIVIT (Clips versus Ligatures in Thyroid Surgery) is an investigator initiated, multicenter, patient-blinded, two-group parallel relevance randomized controlled trial designed by the Study Center of the German Surgical Society. Patients scheduled for elective resection of at least two third of the gland for benign thyroid disease are eligible for participation. After surgical exploration patients are randomized intraoperatively into either the conventional ligature group, or into the clip group. The primary objective is to test for a relevant reduction in operating time (at least 15 min) when using the clip technique. Since April 2004, 121 of the totally required 420 patients were randomized in five centers. DISCUSSION: As in all trials the different forms of bias have to be considered, and as in this case, a surgical trial, the role of surgical expertise plays a key role, and will be documented and analyzed separately. This is the first randomized controlled multicenter relevance trial to compare different vessel occlusion techniques in thyroid surgery with adequate power and other detailed information about the design as well as framework. If significant, the results might be generalized and may change the current surgical practice

Is Adipose Tissue a Place for Mycobacterium tuberculosis Persistence?

BACKGROUND: Mycobacterium tuberculosis, the etiological agent of tuberculosis (TB), has the ability to persist in its human host for exceptionally long periods of time. However, little is known about the location of the bacilli in latently infected individuals. Long-term mycobacterial persistence in the lungs has been reported, but this may not sufficiently account for strictly extra-pulmonary TB, which represents 10–15% of the reactivation cases. METHODOLOGY/PRINCIPAL FINDINGS: We applied in situ and conventional PCR to sections of adipose tissue samples of various anatomical origins from 19 individuals from Mexico and 20 from France who had died from causes other than TB. M. tuberculosis DNA could be detected by either or both techniques in fat tissue surrounding the kidneys, the stomach, the lymph nodes, the heart and the skin in 9/57 Mexican samples (6/19 individuals), and in 8/26 French samples (6/20 individuals). In addition, mycobacteria could be immuno-detected in perinodal adipose tissue of 1 out of 3 biopsy samples from individuals with active TB. In vitro, using a combination of adipose cell models, including the widely used murine adipose cell line 3T3-L1, as well as primary human adipocytes, we show that after binding to scavenger receptors, M. tuberculosis can enter within adipocytes, where it accumulates intracytoplasmic lipid inclusions and survives in a non-replicating state that is insensitive to the major anti-mycobacterial drug isoniazid. CONCLUSIONS/SIGNIFICANCE: Given the abundance and the wide distribution of the adipose tissue throughout the body, our results suggest that this tissue, among others, might constitute a vast reservoir where the tubercle bacillus could persist for long periods of time, and avoid both killing by antimicrobials and recognition by the host immune system. In addition, M. tuberculosis-infected adipocytes might provide a new model to investigate dormancy and to evaluate new drugs for the treatment of persistent infection

Translational toxicology in setting occupational exposure limits for dusts and hazard classification – a critical evaluation of a recent approach to translate dust overload findings from rats to humans

Background We analyze the scientific basis and methodology used by the German MAK Commission in their recommendations for exposure limits and carcinogen classification of “granular biopersistent particles without known specific toxicity” (GBS). These recommendations are under review at the European Union level. We examine the scientific assumptions in an attempt to reproduce the results. MAK’s human equivalent concentrations (HECs) are based on a particle mass and on a volumetric model in which results from rat inhalation studies are translated to derive occupational exposure limits (OELs) and a carcinogen classification. Methods We followed the methods as proposed by the MAK Commission and Pauluhn 2011. We also examined key assumptions in the metrics, such as surface area of the human lung, deposition fractions of inhaled dusts, human clearance rates; and risk of lung cancer among workers, presumed to have some potential for lung overload, the physiological condition in rats associated with an increase in lung cancer risk. Results The MAK recommendations on exposure limits for GBS have numerous incorrect assumptions that adversely affect the final results. The procedures to derive the respirable occupational exposure limit (OEL) could not be reproduced, a finding raising considerable scientific uncertainty about the reliability of the recommendations. Moreover, the scientific basis of using the rat model is confounded by the fact that rats and humans show different cellular responses to inhaled particles as demonstrated by bronchoalveolar lavage (BAL) studies in both species. Conclusion Classifying all GBS as carcinogenic to humans based on rat inhalation studies in which lung overload leads to chronic inflammation and cancer is inappropriate. Studies of workers, who have been exposed to relevant levels of dust, have not indicated an increase in lung cancer risk. Using the methods proposed by the MAK, we were unable to reproduce the OEL for GBS recommended by the Commission, but identified substantial errors in the models. Considerable shortcomings in the use of lung surface area, clearance rates, deposition fractions; as well as using the mass and volumetric metrics as opposed to the particle surface area metric limit the scientific reliability of the proposed GBS OEL and carcinogen classification.International Carbon Black Associatio