Effect of Propolis on Experimental Cutaneous Wound Healing in Dogs

This study evaluates clinically the effect of propolis paste on healing of cutaneous wound in dogs. Under general anesthesia and complete aseptic conditions, two full thickness skin wounds (3 cm diameter) were created in each side of the chest in five dogs, one dorsal and one ventral, with 10 cm between them. These wounds were randomly allocated into two groups, control group (10 wounds) and propolis group (10 wounds). Both groups were represented in each dog. The wounds were cleaned with normal saline solution and dressed with macrogol ointment in control group and propolis paste in propolis group, twice daily till complete wound healing. Measurement of the wound area (cm2) was monitored planimetrically at 0, 7, 14, 21, 28, and 35 days after injury. The data were analyzed statistically. The results revealed a significant reduction in the wound surface area in the propolis group after 14 and 21 days compared to control group. The wound reepithelization, contraction, and total wound healing were faster in propolis group than in control group during five weeks of study. In conclusion, propolis paste has a positive impact on cutaneous wound healing and it may be suggested for treating various types of wounds in animals

Aloe vera in dentistry: Current status and future prospects

This review declares the chemical composition, pharmacological actions, clinical applications, adverse reactions and future prospects of Aloe vera (AV) in the field of dentistry. Literature pertinent to Aloe vera in dental practice was explored in databases like PubMed, Scopus and Google Scholar. The relevant literature was reviewed and critically appraised in this review. Aloe vera is a miracle plant that has been applied as herbal therapy for thousands of years due to its multiple health benefits. In dental practice, AV has different applications and it attracts the attention of researchers due to its natural origin, low cytotoxicity, and various biological actions like antimicrobial, immunomodulatory, anti-inflammatory, antioxidant, wound healing, and antineoplastic actions. Therefore, it has been used for treatment of oral lesions, endodontic lesions, periodontal disease, oral wounds, and alveolar osteitis as well as in tissue engineering, reduction of plaque formation, decontamination of Gutta percha, around dental implants and others. AV has been used in treatment and prevention of several oral and dental problems. Nevertheless, it has a lot to offer in the dental practice and future studies are recommended to maximize its beneficial role in the field of dentistr

Značajke višestrukih falangealnih ozljeda uočene magnetskom rezonancijom (MR) u konja za vuču - prikaz slučaja

Magnetic resonance imaging (MRI) is fast becoming the gold standard diagnostic tool for lameness in equines. This case report describes the MRI features of multiple phalangeal disorders in a 9-year-old horse with chronic unilateral forelimb lameness due to severe trauma. A complete case history was taken, and a thorough clinical examination, radiography, ultrasonography and MRI were performed. Radiography and ultrasonography showed limited value. MRI revealed edema of the navicular bursa (NB), distal sesamoidean impar desmitis, DDFT injury at navicular and post-navicular levels, cartilage erosion of the pastern and coffin joints, medial collateral desmitis of the fetlock joint, medial collateral sesamoidean desmitis, and lateral collateral desmitis of the coffin joint. In conclusion, severe trauma may induce multiple phalangeal disorders with severe pain in draft horses, and MRI is superior to other diagnostic imaging modalities, such as radiography and ultrasonography, for diagnosing such cases.Magnetska rezonancija (MR) ubrzano postaje zlatni standard u dijagnostici hromosti u konja. U ovom se radu opisuju značajke MR-a u dijagnostici višestrukih falangealnih ozljeda u devetogodišnjeg konja s kroničnom unilateralnom hromošću prednjih ekstremiteta uzrokovanom teškom traumom. Provedena je detaljna anamneza, temeljiti klinički pregled, rendgenska pretraga, ultrazvučna i MR pretraga. Rendgenska i ultrazvučna pretraga pokazale su ograničenu vrijednost. MR je otkrio edem navikularne burze (NB), distalni sezamoidni dezmitis, DDFT ozljedu na navikularnoj i postnavikularnoj razini, eroziju hrskavice putičnih i kopitnih zglobova, medijalni kolateralni dezmitis skočnog zgloba, medijalni kolateralni sezamoidni dezmitis i lateralni kolateralni dezmitis kopitnog zgloba. Zaključeno je da teška trauma može uzrokovati višestruke falangealne ozljede s jakom boli u konja za vuču, a MR je superioran ostalim metodama slikovne dijagnostike kao što su rendgenska i ultrazvučna pretraga u dijagnostici ovakvih slučajeva

Utjecaj osteogeničnih dodataka agregatu mineralnog trioksida na razinu genske ekspresije odontoblastičnih markera nakon zatvaranja pulpe u pasa.

This study investigates the effect of addition of dexamethasone, vitamin D3, or chitosan to mineral trioxide aggregate (MTA) on the gene expression level of dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE) after pulp capping in dogs. Pulp exposure was performed in sixty dogs’ teeth. The teeth were classified into 3 equal groups according to the evaluation period. Group 1:7 days, group 2: 21 days and group 3: 60 days. Each group was further subdivided according to the pulp capping material used, into subgroup A: MTA + dexamethasone, subgroup B: MTA + dexamethasone + vitamin D3, subgroup C: MTA + chitosan and subgroup D: MTA. According to the group, the pulps of the capped teeth were removed for analysis of the relative mRNA expression level of DSPP and MEPE using PCR. Statistical analysis of all data was performed. In subgroup A, significant expression was observed of DSPP (P≤0.05) in group 2 up to 18.8 relative fold change while in subgroup B a significant upregulated gene expression of DSPP (P≤0.05) up to 29.4 relative fold change was seen. Significant upregulated DSPP expression (P≤0.05) was recorded in groups 1 and 2 up to 6.9 and 3.6 relative fold change, respectively in subgroup C. In conclusion, dexamethasone, with or without vitamin D3 and chitosan, are synergistic odontogenic inducers with MTA for differentiation of dental pulp cells in dogs. The upregulation of DSPP is a good marker for this differentiation.Istraživan je utjecaj dodatka deksametazona, vitamina D3 ili hitozana agregatu mineralnog trioksida na razinu ekspresije gena za dentin-sijalofosfoprotein (DSPP) i gena za ekstracelularni matriks fosfoglikoprotein nakon zatvaranja pulpe u pasa. Pulpa je bila otvorena na 60 zuba. Ti su zubi bili razvrstani u tri jednake skupine ovisno o trajanju promatranja. Prva skupina bila je promatrana sedam dana, druga skupina 21 dan, a treća skupina 60 dana. Svaka skupina bila je podijeljena u podskupine ovisno o materijalu rabljenom za zatvaranje pulpe. Podskupini A bio je primijenjen agregat mineralnog trioksida + deksametazon, podskupini B bio je primijenjen agregat mineralnog trioksida + deksametazon + vitamin D3, podskupini C agregat mineralnog trioksida + hitozan, a podskupini D samo agregat mineralnog trioksida. Sukladno skupinama, pulpe zatvorenih zuba bile su uzete za analizu relativne ekspresije mRNA za dentin-sijalofosfoprotein i za ekstracelularni matriks fosfoglikoprotein PCR-om. Svi podatci bili su statistički obrađeni. Značajna ekspresija gena za dentinsijalofosfoprotein (P≤0,05) bila je dokazana u podskupini A druge skupine u relativnoj vrijednosti 18,8, dok je u podskupini B značajna ekspresija gena za dentin-sijalofosfoprotein (P≤0,05) bila 29,4 puta veća. Značajno povećana ekspresija DSPP (P≤0,05) u prvoj i drugoj skupini sve do 6,9 odnosno 3,6 puta ustanovljena je u podskupini C. Može se zaključiti da deksametazon (s vitaminom D3 ili bez njega) i hitozan djeluju sinergistički s agregatom mineralnog trioksida kao odontogenični pokretači za diferencijaciju stanica zubne pulpe u pasa. Povećana razina dentin-sijalofosfoproteina dobar je pokazatelj te diferencijacije

Synopsis on the Role of Diode Laser in Root Canal Disinfection

Effective root canal disinfection is necessary for success of endodontic treatment. Traditional chemo-mechanical techniques such as sodium hypochlorite, mechanical preparation and calcium hydroxide used for intracanal disinfection usually fail in cases of biofilm-related infections. Therefore, several new strategies are applied for root canal disinfection. One of these strategies is using of diode laser that has recently gained significant attention in the endodontic practice due to its antibacterial action, bio-stimulation, decrease of pain and improvement of success. Moreover, it has several advantages like its low cost, easily application, penetration power into dentine and smaller size device compared with other types of lasers. Diode laser has been applied for root canal disinfection with various degrees of success in both in vitro and in vivo studies. Nevertheless, small number of published randomized clinical trials in humans with small sample sizes and high heterogeneity provide insufficient support for its efficacy. Furthermore, the results of diode laser in root canal disinfection are controversial. Future high quality studies are recommended to assess the effectiveness of diode laser on various microbial endodontic species and to confirm its role in disinfection of the root canal system in human teeth. This synopsis describes the current scientific understanding and the status of diode laser efficacy in root canal disinfection

Ultrasonographic Diagnosis of Some Scrotal Swellings in Bulls

Information regarding the use of ultrasonography in the diagnosis of testicular and scrotal affections is scarce in bovine. In the present study, eight bulls suffering with scrotal swellings were examined ultrasonographically. The recorded affections included; ruptured urethra with scrotal swelling, testicular hypoplasia, malignant Sertoli cell neoplasm, hydrocele and scrotal hernia. In case of ruptured urethra, the scrotum appeared as thick hyperechoic skin, the testes showed normal echogenicity and the surrounding tissue showed diffuse anechoic areas separated by hyperechoic threads. The hypoplastic testis was less echogenic, and its rete testis was more echogenic, than the normal one. The neoplastic testis had several anechoic areas, hyperechoic masses and areas of normal testicular echogenicity. In hydrocele, the scrotal skin appeared as clear hyperechoic line with anechoic fluid accumulated in the vaginal cavity of the scrotum and both testes had normal echotexture. In scrotal hernia, a hyperechoic omental loop was seen adjacent to high echogenic testis. In conclusion, ultrasonography is a good tool for differential diagnosis of different scrotal swellings in bulls

Effects of Bivariation Viscosity and Magnetic Field on Trapping in a Uniform Tube with Peristalsis

In recent papers, Mehdi Lachiheb has considered fluid viscosity through a peristaltic tube and a channel as a function of the radial and axial components. This author discussed the trapping phenomenon at the centerline of a peristaltic tube and a channel, the pressure rise, and the drag (friction) forces without a magnetic field. Considering the importance of magnetohydrodynamic fluids in bioengineering and medical sciences, we discussed the effects of bivariation viscosity and magnetic field on the trapping phenomenon at the centerline, separated flow on the wall surface of the peristaltic tube, the drag (friction) forces, and the pressure rise. To solve the problem under low Reynolds and long wavelength assumptions, the velocity field and pressure gradient as functions of Hartmann number, amplitude ratio, viscosity parameter, and volume flow rate were obtained using the perturbation approach in terms of Hartmann number (M \u3c 1). The peristaltic pumping and augmented pumping regions were discussed through drag (friction) forces and the pressure rising. In addition, separation flow points on the surface of the wall were determined numerically

Physical properties and bioactivity of Aloe vera modified tricalcium silicate-based cement

Objective: This study compares the physical properties and bioactivity of tricalcium silicate-based cement (Matreva MTA) modified with various concentrations of Aloe vera (AV) solutions and Biodentine cement. Methods: Sixty discs were prepared, and divided into 5 groups (12 discs each) based on the estimated materials: group I: Biodentine, group II: Matreva MTA, group III: Matreva MTA with 10% AV, group IV: Matreva MTA with 20% AV and group V: Matreva MTA with 30% AV. The flow and setting time were assessed following ISO standard 6876:2012 and American Society for Testing and Materials (ASTM) standard C266-21, respectively. The in-vitro bioactivity was evaluated after 1, 14, and 28 days of immersion in Hank\u27s balanced salt solution (HBSS) including pH, calcium ion release, and apatite formation. All data were statistically analyzed. Results: Increasing the AV concentration added to Matreva MTA from 0 to 30% decreased the flow and setting time. Significant high mean pH and calcium ion release values were observed for 20 and 30% AV-modified Matreva MTA cement at 14 and 28 days (P≤ 0.05). Environmental scanning electron microscope/ energy-dispersive X-ray spectroscopy (ESEM/EDX) analysis confirmed calcium phosphate nucleation on the surfaces of 20 and 30% AV-modified Matreva MTA cements after immersion in HBSS. Conclusion: Addition of 20 and 30% AV solutions to Matreva MTA reduced the setting time and improved the handling characteristics as well as the in-vitro bioactivity, resembling the qualities of Biodentine. Both AV-modified Matreva MTA and Biodentine cements had extended alkalinizing activity and calcium ion release. Clinical relevance: AV-modified Matreva MTA can be considered a promising biomaterial for different endodontic applications