4 research outputs found
Bioenergy Technologies for a Net Zero Transition:Outcomes of UK-India Bioenergy Research Scoping
The report is part of scoping exercise led by UK Research and Innovation (UKRI)’s Engineering and Physical Sciences Research Council (EPSRC) and Biotechnology and Biological Sciences Research Council (BBSRC) and commissioned to Supergen Bioenergy Hub. The report is for UKRI, funded by UKRI India. UKRI launched in April 2018. UKRI is a non-departmental public body sponsored by the Department for Business, Energy and Industrial Strategy (BEIS). Our organisation brings together the seven disciplinary research councils, Research England, which is responsible for supporting research and knowledge exchange at higher education institutions in England, and the UK’s innovation agency, Innovate UK. Our nine councils work together in innovative ways to deliver an ambitious agenda, drawing on our great depth and breadth of expertise and the enormous diversity of our portfolio. http://www.ukri.org UKRI India plays a key role in enhancing the research and innovation collaboration between the UK and India. Since 2008, the UK and Indian governments, and third parties, have together invested over £330 million in co-funded research and innovation programmes. This investment has brought about more than 258 individual projects. The projects were funded by over 15 funding agencies, bringing together more than 220 lead institutions from the UK and India. These research projects have generated more than £450 million in further funding, mainly from public bodies but also from non-profit organisations and commercial entities, attesting the relevance of these projects. www.ukri.org/india This work was commissioned to inform UKRI/UKRI India priorities and pathways for innovation development in bioenergy with UK-India partnerships
HILIC- and SCX-Based Quantitative Proteomics of <i>Chlamydomonas reinhardtii</i> during Nitrogen Starvation Induced Lipid and Carbohydrate Accumulation
Nitrogen starvation induced changes in carbohydrate and
lipid content is described in several algal species. Although these
phenotypic changes are desirable, such manipulations also significantly
deteriorate culture health, ultimately halting growth. To optimize
biofuel production from algae, it is desirable to induce lipid accumulation
without compromising cell growth and survival. In this study, we utilized
an 8-plex iTRAQ-based proteomic approach to assess the model alga <i>Chlamydomonas reinhardtii</i> CCAP 11/32CW15+ under nitrogen
starvation. First-dimension fractionation was conducted using HILIC
and SCX. A total of 587 proteins were identified (≥3 peptides)
of which 71 and 311 were differentially expressed at significant levels
(<i>p</i> < 0.05), during nitrogen stress induced carbohydrate
and lipid production, respectively. Forty-seven percent more changes
with significance were observed with HILIC compared to SCX. Several
trends were observed including increase in energy metabolism, decrease
in translation machinery, increase in cell wall production and a change
of balance between photosystems I and II. These findings point to
a severely compromised system where lipid is accumulated at the expense
of normal functioning of the organism, suggesting that a more informed
and controlled method of lipid induction than gross nutrient manipulation
would be needed for development of sustainable processes
HILIC- and SCX-Based Quantitative Proteomics of <i>Chlamydomonas reinhardtii</i> during Nitrogen Starvation Induced Lipid and Carbohydrate Accumulation
Nitrogen starvation induced changes in carbohydrate and
lipid content is described in several algal species. Although these
phenotypic changes are desirable, such manipulations also significantly
deteriorate culture health, ultimately halting growth. To optimize
biofuel production from algae, it is desirable to induce lipid accumulation
without compromising cell growth and survival. In this study, we utilized
an 8-plex iTRAQ-based proteomic approach to assess the model alga <i>Chlamydomonas reinhardtii</i> CCAP 11/32CW15+ under nitrogen
starvation. First-dimension fractionation was conducted using HILIC
and SCX. A total of 587 proteins were identified (≥3 peptides)
of which 71 and 311 were differentially expressed at significant levels
(<i>p</i> < 0.05), during nitrogen stress induced carbohydrate
and lipid production, respectively. Forty-seven percent more changes
with significance were observed with HILIC compared to SCX. Several
trends were observed including increase in energy metabolism, decrease
in translation machinery, increase in cell wall production and a change
of balance between photosystems I and II. These findings point to
a severely compromised system where lipid is accumulated at the expense
of normal functioning of the organism, suggesting that a more informed
and controlled method of lipid induction than gross nutrient manipulation
would be needed for development of sustainable processes
Bioenergy technologies for a net zero transition: outcomes of UK-India bioenergy research scoping
Bioenergy technologies for a net zero transition: outcomes of UK-India bioenergy research scoping</p