Data_Sheet_2_Intestinal epithelial cell-derived components regulate transcriptome of Lactobacillus rhamnosus GG.PDF

IntroductionIntestinal epithelial cells (IECs) provide the frontline responses to the gut microbiota for maintaining intestinal homeostasis. Our previous work revealed that IEC-derived components promote the beneficial effects of a commensal and probiotic bacterium, Lactobacillus rhamnosus GG (LGG). This study aimed to elucidate the regulatory effects of IEC-derived components on LGG at the molecular level.MethodsDifferential gene expression in LGG cultured with IEC-derived components at the timepoint between the exponential and stationary phase was studied by RNA sequencing and functional analysis.ResultsThe transcriptomic profile of LGG cultured with IEC-derived components was significantly different from that of control LGG, with 231 genes were significantly upregulated and 235 genes significantly down regulated (FDR DiscussionThese results provide mechanistic insight into the interactions between the gut microbiota and the host.</p

Data_Sheet_3_Intestinal epithelial cell-derived components regulate transcriptome of Lactobacillus rhamnosus GG.PDF

IntroductionIntestinal epithelial cells (IECs) provide the frontline responses to the gut microbiota for maintaining intestinal homeostasis. Our previous work revealed that IEC-derived components promote the beneficial effects of a commensal and probiotic bacterium, Lactobacillus rhamnosus GG (LGG). This study aimed to elucidate the regulatory effects of IEC-derived components on LGG at the molecular level.MethodsDifferential gene expression in LGG cultured with IEC-derived components at the timepoint between the exponential and stationary phase was studied by RNA sequencing and functional analysis.ResultsThe transcriptomic profile of LGG cultured with IEC-derived components was significantly different from that of control LGG, with 231 genes were significantly upregulated and 235 genes significantly down regulated (FDR DiscussionThese results provide mechanistic insight into the interactions between the gut microbiota and the host.</p

Data_Sheet_1_Intestinal epithelial cell-derived components regulate transcriptome of Lactobacillus rhamnosus GG.PDF

IntroductionIntestinal epithelial cells (IECs) provide the frontline responses to the gut microbiota for maintaining intestinal homeostasis. Our previous work revealed that IEC-derived components promote the beneficial effects of a commensal and probiotic bacterium, Lactobacillus rhamnosus GG (LGG). This study aimed to elucidate the regulatory effects of IEC-derived components on LGG at the molecular level.MethodsDifferential gene expression in LGG cultured with IEC-derived components at the timepoint between the exponential and stationary phase was studied by RNA sequencing and functional analysis.ResultsThe transcriptomic profile of LGG cultured with IEC-derived components was significantly different from that of control LGG, with 231 genes were significantly upregulated and 235 genes significantly down regulated (FDR DiscussionThese results provide mechanistic insight into the interactions between the gut microbiota and the host.</p

The Judicial Activity of the International Court of Justice in 2015

Human-F. tularensis protein-protein interactions retested in pairwise Y2H experiments. (DOCX 20 kb

Additional file 2: PPI data. of Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors

F. tularensis protein interactions with 1) human proteins, 2) murine proteins, and 3) the combined set of human proteins augmented with murine orthologs. (XLSX 28 kb

Additional file 5: Figure S1. of Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors

Evaluation of the effect of five mutants on Francisella tularensis virulence using mouse intranasal model experiments. (DOCX 372 kb

Additional file 1: Table S1. of Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors

Francisella strains used to identify proteins overrepresented in the pathogenic strains. (DOCX 27 kb

Additional file 2: Figure S1. of Gastrointestinal microbial populations can distinguish pediatric and adolescent Acute Lymphoblastic Leukemia (ALL) at the time of disease diagnosis

Box-plots of the alpha diversity of OTUs at 95 % and 90 % identity threshold of the Control and Patient groups. The Patient group is further partitioned into the group taking antibiotics 1-month period Visit 1 (Patient_A) and the group not taking antibiotics (Patient_NA). (A) Alpha diversity for 95 % OTUs, the Y-axis denotes alpha diversity (Shannon Index values). The Wilcoxon Rank Sum test p-value = 0.00253 for Control vs Patient, p-value = 0.00328 for Control vs Patient_A and p-value = 0.05969 for Control vs Patient_NA. (B) Alpha diversity for 90 % OTUs, the Y-axis denotes alpha diversity (Shannon Index values). The Wilcoxon Rank Sum test p-value = 0.00156 for Control vs Patient, p-value = 0.00328 for Control vs Patient_A and p-value = 0.03119 for Control vs Patient_NA. In both cases (A, B) the Patient group has a lower microbiota diversity (statistically significant) compared to the Control group (p-value < 0.0026). The diversities of the Patient_A and Patient_NA groups are also significantly lower (p-value < 0.05) than the Control group. (PDF 203 kb

Additional file 1: Table S1. of Gastrointestinal microbial populations can distinguish pediatric and adolescent Acute Lymphoblastic Leukemia (ALL) at the time of disease diagnosis

List of fecal samples collected for each patient over the period of enrollment for 16S rRNA gene sequencing and analysis. (XLSX 17 kb