213 research outputs found
Quantum Mechanical Aspects of Cell Microtubules: Science Fiction or Realistic Possibility?
Recent experimental research with marine algae points towards quantum
entanglement at ambient temperature, with correlations between essential
biological units separated by distances as long as 20 Angstr\"oms. The
associated decoherence times, due to environmental influences, are found to be
of order 400 fs. This prompted some authors to connect such findings with the
possibility of some kind of quantum computation taking place in these
biological entities: within the decoherence time scales, the cell "quantum
calculates" the optimal "path" along which energy and signal would be
transported more efficiently. Prompted by these experimental results, in this
talk I remind the audience of a related topic proposed several years ago in
connection with the possible r\^ole of quantum mechanics and/or field theory on
dissipation-free energy transfer in microtubules (MT), which constitute
fundamental cell substructures. Quantum entanglement between tubulin dimers was
argued to be possible, provided there exists sufficient isolation from other
environmental cell effects. The model was based on certain ferroelectric
aspects of MT. In the talk I review the model and the associated experimental
tests so far and discuss future directions, especially in view of the algae
photo-experiments.Comment: 31 pages latex, 11 pdf figures, uses special macros, Invited Plenary
Talk at DICE2010, Castello Pasquini, Castiglioncello (Italy), September 13-18
201
A clinically relevant sheep model of orthotopic heart transplantation 24Â h after donor brainstem death
BACKGROUND: Heart transplantation (HTx) from brainstem dead (BSD) donors is the gold-standard therapy for severe/end-stage cardiac disease, but is limited by a global donor heart shortage. Consequently, innovative solutions to increase donor heart availability and utilisation are rapidly expanding. Clinically relevant preclinical models are essential for evaluating interventions for human translation, yet few exist that accurately mimic all key HTx components, incorporating injuries beginning in the donor, through to the recipient. To enable future assessment of novel perfusion technologies in our research program, we thus aimed to develop a clinically relevant sheep model of HTx following 24 h of donor BSD. METHODS: BSD donors (vs. sham neurological injury, 4/group) were hemodynamically supported and monitored for 24 h, followed by heart preservation with cold static storage. Bicaval orthotopic HTx was performed in matched recipients, who were weaned from cardiopulmonary bypass (CPB), and monitored for 6 h. Donor and recipient blood were assayed for inflammatory and cardiac injury markers, and cardiac function was assessed using echocardiography. Repeated measurements between the two different groups during the study observation period were assessed by mixed ANOVA for repeated measures. RESULTS: Brainstem death caused an immediate catecholaminergic hemodynamic response (mean arterial pressure, p = 0.09), systemic inflammation (IL-6 - p = 0.025, IL-8 - p = 0.002) and cardiac injury (cardiac troponin I, p = 0.048), requiring vasopressor support (vasopressor dependency index, VDI, p = 0.023), with normalisation of biomarkers and physiology over 24 h. All hearts were weaned from CPB and monitored for 6 h post-HTx, except one (sham) recipient that died 2 h post-HTx. Hemodynamic (VDI - p = 0.592, heart rate - p = 0.747) and metabolic (blood lactate, p = 0.546) parameters post-HTx were comparable between groups, despite the observed physiological perturbations that occurred during donor BSD. All p values denote interaction among groups and time in the ANOVA for repeated measures. CONCLUSIONS: We have successfully developed an ovine HTx model following 24 h of donor BSD. After 6 h of critical care management post-HTx, there were no differences between groups, despite evident hemodynamic perturbations, systemic inflammation, and cardiac injury observed during donor BSD. This preclinical model provides a platform for critical assessment of injury development pre- and post-HTx, and novel therapeutic evaluation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40635-021-00425-4
Clinical Efficacy of Attentional Bias Modification Procedures: An Updated Meta-Analysis
The Influence of Law and Economics Scholarship on Contract Law: Impressions Twenty-Five Years Later
Potential Liability for Mortgage Lenders and Servicers Under HAMP and State Consumer Protection Laws During the Mortgage Modification Process
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