Preliminary measurements of lumbar spine kinematics and stiffness

The purpose of the presented study was the experimental measurement of lumbar spine stiffness and its range of motion. The dependence of torsion moment of lumbar spine segment on deflection of flexion, extension and torsion was observed during experiments. Stiffness of spine segment was determined from measured data. Human lumbar spine was used for verification of the experimental technique. The sample consisted of one lumbar vertebrae composed by five vertebral bodies and four intervertebral discs. All muscles were removed, however all ligaments were preserved. Experiments were ca rried out on the test system MTS 858.2 MiniBionix, where loading by axial force and torsion moment is possible at the same time. Special Modular Bionix Spine Test Fixator, attached to the test system was used for the measurements. Loading was controlled kinematically (gradual turning) by keeping the axial force equal zero. Measurement was timedependent. The results of these experiments are going to be used as input data for creating a model of artificial lumbar spine and new type of artificial disc replacement

Achromobacter marplatensis sp. nov., isolated from a pentachlorophenol contaminated soil

A polyphasic taxonomic approach was applied to the study of a Gram-negative bacterium (B2T) isolated from soil by selective enrichment with pentachlorophenol. 16S rRNA gene sequence analysis of strain B2T showed that the strain belongs to the genus Achromobacter within the Betaproteobacteria. The 16S rRNA gene sequence displayed more than 99 % similarity to the sequences of the type strains of all species of Achromobacter, with the highest sequence similarity to those of Achromobacter spanius CCM 7183T and A. piechaudii CCM 2986T (99.8 %). On the basis of phylogenetic analysis, genomic DNA–DNA relatedness and phenotypic characteristics, including chemotaxonomic (cellular fatty acid profile) analysis, a novel species is proposed, Achromobacter marplatensis sp. nov., with the type strain B2T ( = CCM 7608T = CCUG 56371T = CECT 7342T)