DESCRIPTION OF TYLENCHORHYNCHUS IRANENSIS SP. N. (NEMATODA TELOTYLENCHIDAE) FROM IRAN

A new species of stunt nematodes, Tylenchorhynchus iranensis sp. n. is described from the rhizosphere of faba bean (Vicia faba L.), from material collected in the Khuzestan province, southwestern Iran. The new species is characterized by the following combination of features: lateral fields with four non areolated incisures, cephalic region continuous to slightly offset and conformed by 6-7 fine annuli, stylet 15-18 µm long, post-anal intestinal sac extends into the entire tail cavity, epiptygma present, tail sub-cylindrical with a hemispherical to sub-hemispherical smooth terminus, 46-65 μm long and composed by 46-49 annuli. Molecular analysis based on 28S rRNA gene sequences placed T. iranensis sp. n. within a clade that contained representatives of the genus Tylenchorhynchus with high support

DESCRIPTION OF TYLENCHORHYNCHUS IRANENSIS SP. N. (NEMATODA TELOTYLENCHIDAE) FROM IRAN

A new species of stunt nematodes, Tylenchorhynchus iranensis sp. n. is described from the rhizosphere of faba bean (Vicia faba L.), from material collected in the Khuzestan province, southwestern Iran. The new species is characterized by the following combination of features: lateral fields with four non areolated incisures, cephalic region continuous to slightly offset and conformed by 6-7 fine annuli, stylet 15-18 µm long, post-anal intestinal sac extends into the entire tail cavity, epiptygma present, tail sub-cylindrical with a hemispherical to sub-hemispherical smooth terminus, 46-65 μm long and composed by 46-49 annuli. Molecular analysis based on 28S rRNA gene sequences placed T. iranensis sp. n. within a clade that contained representatives of the genus Tylenchorhynchus with high support

MORPHOLOGICAL, MORPHOMETRIC AND MOLECULAR CHARACTERIZATION OF MERLINIUS MICRODORUS (GERAERT, 1966) SIDDIQI, 1970, SCUTYLENCHUS RUGOSUS (SIDDIQI, 1963) SIDDIQI, 1979 (MERLINIIDAE), AND PSILENCHUS CURCUMERUS RAHAMAN, AHMAD AND JAIRAJPURI, 1994 (PSILENCHIDAE) AND APPROACHES TO PHYLOGENETIC RELATIONSHIPS

Merlinius microdorus and Scutylenchus rugosus (Merliniidae), and Psilenchus curcumerus (Psilenchidae) were collected from the rhizosphere of faba bean (Vicia faba L.) fields in Khuzestan province, south-western Iran. Morphological and morphometric data are provided for these species. Additionally, sequences of the D2-D3 expansion segments of 28S rRNA gene for all species were also used for molecular phylogenetic analysis. The phylogenetic relationships of Psilenchidae and Merliniidae in relation to representatives of the superfamily Tylenchoidea, obtained from Bayesian inference (BI) and maximum likelihood (ML) analyses of the D2-D3 sequences, are presented and discussed. The results of phylogenetic analysis strongly supported (BPP = 100) Merliniidae and Psilenchidae as monophyletic. The family Tylenchidae formed a sister clade to Merliniidae/Psilenchidae with high branch support (BPP = 100). Monophyly of representatives of Merliniidae (including Pratylenchoides) was supported with maximum BPP

First Morphological and Molecular Characterization of Paratylenchus vandenbrandei (Rhabditida: Tylenchulidae) in Iran

Paratylenchus vandenbrandei, has been recovered from the rhizospheric soil of Euphrates poplar (Populus euphratica) in the Karkheh protected area of Khuzestan province, southwestern Iran. The species was identified as P. vandenbrandei by the presence of three lines in the lateral fields; conoid rounded lip region; presence of submedian lobes, a stylet 24.0–28.8 μm long; an excretory pore at the level of the anterior part of the pharyngeal bulb; a round-to-oval spermatheca; presence of vulval flaps; and a conoid tail, with a terminus that is rounded or slightly pointed in some specimens. Males have a conoid tail, with a rounded-to-slightly-pointed terminus. The phylogenetic relationships of the species were reconstructed and investigated using partial sequencing of the D2-D3 expansion segments of large subunits, as well as internal transcribed spacer regions (LSU D2-D3 and ITS rDNA) based on Bayesian inference (BI). P. vandenbrandei has formed a clade with P. neonanus, P. minor, P. nainianus, P. chongqinjensis, P. pedrami, P. baldaccii, P. leptos and P. rostrocaudatus with maximal support (BPP = 1.00). To the best of our knowledge, this is the first report of P. vandenbrandei in Iran and the first molecular characterization of the species worldwide

Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family

Eisvand, Payam, Nejad, Reza Farrokhi, Azimi, Sedighe (2019): Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family. Zootaxa 4563 (3): 482-490, DOI: https://doi.org/10.11646/zootaxa.4563.3.

FIGURE 2. Basiria khouzestanensis n in Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family

FIGURE 2. Basiria khouzestanensis n. sp. A&B: Female pharyngeal region, C: Lateral field at mid-body, D: Female reproductive system, E&F: Female tail, G&H: Male tail. (Scale bars = 20 µm.