14 research outputs found
Bicyclic Isoxazoline Derivatives: Synthesis and Evaluation of Biological Activity
The application of non-planar scaffolds in drug design allows for the enlargement of the chemical space, and for the construction of molecules that have more effective target–ligand interactions or are less prone to the development of resistance. Among the works of the last decade, a literature search revealed spirothiazamenthane, which has served as a lead in the development of derivatives active against resistant viral strains. In this work, we studied the novel molecular scaffold, which resembles spirothiazamenthane, but combines isoxazoline as a heterocycle and cyclooctane ring as a hydrophobic part of the structure. The synthesis of new 3-nitro- and 3-aminoisoxazolines containing spiro-fused or 1,2-annelated cyclooctane fragments was achieved by employing 1,3-dipolar cycloaddition of 3-nitro-4,5-dihydroisoxazol-4-ol 2-oxide or tetranitromethane-derived alkyl nitronates with non-activated alkenes. A series of spiro-sulfonamides was obtained by the reaction of 3-aminoisoxazoline containing a spiro-fused cyclooctane residue with sulfonyl chlorides. Preliminary screening of the compounds for antiviral, antibacterial, antifungal and antiproliferative properties in vitro revealed 1-oxa-2-azaspiro[4.7]dodec-2-en-3-amine and 3a,4,5,6,7,8,9,9a-octahydrocycloocta[d]isoxazol-3-amine with activity against the influenza A/Puerto Rico/8/34 (H1N1) virus in the submicromolar range, and high values of selectivity index. Further study of the mechanism of the antiviral action of these compounds, and the synthesis of their analogues, is likely to identify new agents against resistant viral strains
Bis(oxiranes) Containing Cyclooctane Core: Synthesis and Reactivity towards NaN<sub>3</sub>
Reactions of oxirane ring opening provide a powerful tool for regio- and stereoselective synthesis of polyfunctional and heterocyclic compounds, widely used in organic chemistry and drug design. Cyclooctane, alongside other medium-sized rings, is of interest as a novel molecular platform for the construction of target-oriented leads. Additionally, cyclooctane derivatives are well known to be prone to transannular reactions, which makes them a promising object in the search for novel approaches to polycyclic structures. In the present work, a series of cyclooctanediones was studied in Corey-Chaykovsky reactions, and novel spirocyclic bis(oxiranes) containing cyclooctane core, namely, 1,5-dioxadispiro[2.0.2.6]dodecane and 1,8-dioxadispiro[2.3.2.3]dodecane, were synthesized. Ring opening of the obtained bis(oxiranes) upon treatment with sodium azide was investigated, and it was found that the reaction path is determined by the reciprocal orientation of oxygen atoms in the oxirane moieties. Diastereomers of the bis(oxiranes) with cis-orientation underwent independent ring opening, supplying corresponding diazidodiols, while in the case of stereoisomers with trans-orientation, domino-like reactions occurred, including intramolecular nucleophilic attack and the formation of a novel three- or six-membered O-containing ring. Summarily, a straightforward approach to polyfunctional compounds containing cyclooctane or oxabicyclo[3.3.1]nonane cores, employing bis(oxiranes), was elaborated
Three-Component Heterocyclization of <i>gem</i>-Bromofluorocyclopropanes with NOBF<sub>4</sub>: Access to 4‑Fluoropyrimidine <i>N</i>‑Oxides
Novel three-component heterocyclization involving <i>gem</i>-bromofluorocyclopropanes, nitrosyl tetrafluoroborate,
and a molecule
of the solvent (nitrile) yielding previously unknown fluorinated pyrimidine <i>N</i>-oxides is described. A two-step synthetic approach to
4-fluoropyrimidine <i>N</i>-oxides from alkenes under mild
conditions is developed using this reaction. Mechanistic aspects of
the heterocyclization are discussed
Three-Component Heterocyclization of <i>gem</i>-Bromofluorocyclopropanes with NOBF<sub>4</sub>: Access to 4‑Fluoropyrimidine <i>N</i>‑Oxides
Novel three-component heterocyclization involving <i>gem</i>-bromofluorocyclopropanes, nitrosyl tetrafluoroborate,
and a molecule
of the solvent (nitrile) yielding previously unknown fluorinated pyrimidine <i>N</i>-oxides is described. A two-step synthetic approach to
4-fluoropyrimidine <i>N</i>-oxides from alkenes under mild
conditions is developed using this reaction. Mechanistic aspects of
the heterocyclization are discussed
Three-Component Heterocyclization of <i>gem</i>-Bromofluorocyclopropanes with NOBF<sub>4</sub>: Access to 4‑Fluoropyrimidine <i>N</i>‑Oxides
Novel three-component heterocyclization involving <i>gem</i>-bromofluorocyclopropanes, nitrosyl tetrafluoroborate,
and a molecule
of the solvent (nitrile) yielding previously unknown fluorinated pyrimidine <i>N</i>-oxides is described. A two-step synthetic approach to
4-fluoropyrimidine <i>N</i>-oxides from alkenes under mild
conditions is developed using this reaction. Mechanistic aspects of
the heterocyclization are discussed
Beyond the Dimer and Trimer: Tetraspiro[2.1.25.1.29.1.213.13] hexadecane-1,3,5,7-tetraone-the Cyclic Tetramer of Carbonylcyclopropane
Novel Nanomolar Allosteric Modulators of AMPA Receptor of Bis(pyrimidine) Series: Synthesis, Biotesting and SAR Analysis
Positive allosteric modulators (PAMs) of AMPA receptors represent attractive candidates for the development of drugs for the treatment of cognitive and neurodegenerative disorders. Dimeric molecules have been reported to have an especially potent modulating effect, due to the U-shaped form of the AMPA receptor’s allosteric binding site. In the present work, novel bis(pyrimidines) were studied as AMPA receptor modulators. A convenient and flexible preparative approach to bis(pyrimidines) containing a hydroquinone linker was elaborated, and a series of derivatives with varied substituents was obtained. The compounds were examined in the patch clamp experiments for their influence on the kainate-induced currents, and 10 of them were found to have potentiating properties. The best potency was found for 2-methyl-4-(4-((2-methyl-5,6,7,8-tetrahydroquinazolin-4-yl)oxy)phenoxy)-6,7,8,9-tetrahydro-5H-cyclohepta[d]pyrimidine, which potentiated the kainate-induced currents by up to 77% in all tested concentrations (10−12–10−6 M). The results were rationalized via the modeling of modulator complexes with the dimeric ligand binding domain of the GluA2 AMPA receptor, using molecular docking and molecular dynamics simulation. The prediction of ADMET, physicochemical, and PAINS properties of the studied bis(pyrimidines) confirmed that PAMs of this type may act as the potential lead compounds for the development of neuroprotective drugs
A Facile Approach to Bis(isoxazoles), Promising Ligands of the AMPA Receptor
A convenient synthetic approach to novel functionalized bis(isoxazoles), the promising bivalent ligands of the AMPA receptor, was elaborated. It was based on the heterocyclization reactions of readily available electrophilic alkenes with the tetranitromethane-triethylamine complex. The structural diversity of the synthesized compounds was demonstrated. In the electrophysiological experiments using the patch clamp technique on Purkinje neurons, the compound 1,4-phenylenedi(methylene)bis(5-aminoisoxazole-3-carboxylate) was shown to be highly potent positive modulator of the AMPA receptor, potentiating kainate-induced currents up to 70% at 10−11 M