Identification of Oplophorus Gracilirostris from South-East and South-West Coast of India using Mitochondrial Genetic Marker

The present study reports the occurrence of Oplophorus gracilirostris A. Milne-Edwards, 1881 from southeast and south-west coast of India on the basis of morphological as well as molecular data. It is the first report of insight into the mitochondrial genetic variation of O. gracilirostris. For this study, the DNA sequences of three mitochondrial genes, large subunit ribosomal DNA (16S rDNA), cytochrome oxidase I (COI) and cytochrome b (Cyt b) were submitted in the GenBank. Morphological examination of the specimen confirmed the identity of the species as O. gracilirostris. The pairwise genetic distance analysis, sequence identity and phylogenetic tree have shown that the specimen has identity with O. gracilirostris only on the basis of 16S rDNA sequence of earlier reports. In the case of COI it has shown some identity towards Oplophorus typus and Oplophorus spinosus, but with a high genetic difference. This is the first submission of Cyt b sequence of O. gracilirostris. This study with sequence data of O. gracilirostris in GenBank will provide molecular support to the taxonomical identifications carried out on morphological basi

Rare occurrence of blunthorn lobster Palinustus waguensis Kubo, 1963 from the southwest coast of India

On 12th September, 2013 three specimens of the rare deep-sea blunt horn lobsters were landed in the multiday trawlers operating at a depth of 150-250 m, off Sakthikulangara along the southwest coast of India. These three specimens consisted of two males and a single female

Different Types of Connected Spaces

International audienceThis paper deals with the various forms of open sets and their relations. The relation is represented by a diagram. The relation is same for the closed sets also. The main part defines different types of connectedness and their relations. It is notable that openness and connectedness are in opposite order. Some results on Q-sets are also included here

PENDANT NUMBER OF GRAPHS

International audienceA decomposition of a graph G is a collection of its edge disjoint sub-graphs such that their union is G. A path decomposition of a graph is a decomposition of it into paths. In this paper, we define the pendant number Π p as the minimum number of end vertices of paths in a path decomposition of G and determine this parameter for certain fundamental graph classes

Different Types of Connected Spaces

This paper deals with the various forms of open sets and their relations. The relation is represented by a diagram. The relation is same for the closed sets also. The main part defines different types of connectedness and their relations. It is notable that openness and connectedness are in opposite order. Some results on Q-sets are also included here

A study on the pendant number of graph products

A path decomposition of a graph is a collection of its edge disjoint paths whose union is G. The pendant number Πp is the minimum number of end vertices of paths in a path decomposition of G. In this paper, we determine the pendant number of corona products and rooted products of paths and cycles and obtain some bounds for the pendant number for some specific derived graphs. Further, for any natural number n, the existence of a connected graph with pendant number n has also been established

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Not AvailableThe present study reports the occurrence of Oplophorus gracilirostris A. Milne-Edwards, 1881 from southeast and south-west coast of India on the basis of morphological as well as molecular data. It is the first report of insight into the mitochondrial genetic variation of O. gracilirostris. For this study, the DNA sequences of three mitochondrial genes, large subunit ribosomal DNA (16S rDNA), cytochrome oxidase I (COI) and cytochrome b (Cyt b) were submitted in the GenBank. Morphological examination of the specimen confirmed the identity of the species as O. gracilirostris. The pairwise genetic distance analysis, sequence identity and phylogenetic tree have shown that the specimen has identity with O. gracilirostris only on the basis of 16S rDNA sequence of earlier reports. In the case of COI it has shown some identity towards Oplophorus typus and Oplophorus spinosus, but with a high genetic difference. This is the first submission of Cyt b sequence of O. gracilirostris. This study with sequence data of O. gracilirostris in GenBank will provide molecular support to the taxonomical identifications carried out on morphological basisNot Availabl

Morphological analysis and molecular phylogeny of Aristeus alcocki Ramadan, 1938 from south-west coast of India

Morphological analysis and molecular phylogeny of Aristeus alcocki Ramadan, 1938 from south-west coast of Indi

Morphological analysis and molecular phylogeny of Aristeus alcocki Ramadan, 1938 from south-west coast of India

1716-1725Present study has combined both morphometric as well as molecular phylogenetic analysis to describe Aristeus alcocki on the basis of the specimens procured off Sakthikulangara along the south west coast of India. Morphometric analysis of A. alcocki was confirmed by the relative length of the chela/carpus ratio as P1: 1.14-1.35, P2: 0.95-1.07, P3: 0.7-0.85. Pleurobranchia of pereopod I-IV in the form of minute papillae without pinnules. Hepatic groove well formed straight and anterior part slightly curved. Strong buttress with well developed pterygostomian spine anteriorly curved. Molecular phylogeny of the species was analyzed using two nuclear-protein coding genes, phosphoenolpyruvate carboxykinase (PEPCK) and sodium–potassium ATPase α-subunit (NaK) and two mitochondrial genes, large subunit ribosomal DNA (16S rDNA) and Cytochrome oxidase I (COI). These four highly conserved genes were sequenced and phylogenetic analysis was performed. From this study the specimen was identified as A. alcocki and the molecular data submitted in GenBank will be a contribution towards identification of the species using molecular tools in future

Nuclear and mitochondrial DNA markers based identification of blunthorn lobster <i>Palinustus waguensis</i> Kubo, 1963 from South-west coast of India

172-177The present study reports the occurrence of Palinustus waguensis Kubo, 1963 from South-west coast of India on the basis of morphological as well as molecular data. The DNA sequences of two mitochondrial genes, large subunit ribosomal DNA (16S rDNA) and cytochrome oxidase I (COI), and two nuclear-protein coding genes, viz., phosphoenolpyruvate carboxykinase (PEPCK) and sodium-potassium ATPase α-subunit (NaK), were submitted in the GenBank. Morphological examination of the specimen confirmed the identity of the species as P. waguensis. The pairwise genetic distance analysis, sequence identity and phylogenetic tree have shown that the specimen has similarity only with 16S rDNA sequence of P. waguensis of earlier reports. In the case of other genetic markers, it has shown some identity towards P. unicornutus and P. holthuisi, but with a high genetic difference. This study with sequence data of P. waguensis in GenBank will help to identify the specimen on molecular basis in future