Evoking Places from Spaces: The application of multimodal narrative techniques in the creation of "U-Modified"

Multimodal diegetic narrative tools, as applied in multimedia arts practices, possess the ability to cross the spaces that exist between the physical world and the imaginary. Within this paper we present the findings of a multidiscipline practice-based research project that explored the potential of an audio-visual art performance to purposefully interact with an audience’s perception of narrative place. To achieve this goal, research was undertaken to investigate the function of multimodal diegetic practices as applied in the context of a sonic-art narrative. This project direction was undertaken to facilitate the transformation of previous experiences of place through the creative amalgamation and presentation of collected audio and visual footage from real-world spaces. Through the presentation of multimedia relating to familiar geographical spatial features, the audience were affected to evoke memories of place and to construct and manipulate their own narrative

Evoking Places from Spaces: The application of multimodal narrative techniques in the creation of "U-Modified"

Multimodal diegetic narrative tools, as applied in multimedia arts practices, possess the ability to cross the spaces that exist between the physical world and the imaginary. Within this paper we present the findings of a multidiscipline practice-based research project that explored the potential of an audio-visual art performance to purposefully interact with an audience’s perception of narrative place. To achieve this goal, research was undertaken to investigate the function of multimodal diegetic practices as applied in the context of a sonic-art narrative. This project direction was undertaken to facilitate the transformation of previous experiences of place through the creative amalgamation and presentation of collected audio and visual footage from real-world spaces. Through the presentation of multimedia relating to familiar geographical spatial features, the audience were affected to evoke memories of place and to construct and manipulate their own narrative

Validation of the Static Load Test for Event Detection During Hands-Free Conversation

Objective. To see if visual event reaction times (RTs) during handsfree conversation conditions in the Enhanced Static Load Test (ESLT) can predict RTs in similar conditions in on-road driving. Methods. Brake reaction times to random center and side light events were measured while watching a driving video, attempting to keep a marker in the center of the lane with a steering wheel, answering the phone by pressing a button, and carrying on neutral or angry handsfree conversations in covert (silent) or overt mode on a hands-free phone device. Open-road tests were conducted in traffic for subjects with similar side and front light events, with foot reaction times measured while engaged in the same secondary tasks and conditions. Results. Mean RTs for the task segments in the lab were predictive of the mean RTs for the corresponding task segments in the on-road test (r = 0.90, df = 16, p \u3c 0.000001). Conclusion. This study validates the Enhanced Static Load Test as predictive of visual event RTs during open-road driving for the range of experimental conditions and tasks considered

NELFE-Dependent MYC Signature Identifies a Unique Cancer Subtype in Hepatocellular Carcinoma.

The MYC oncogene is dysregulated in approximately 30% of liver cancer. In an effort to exploit MYC as a therapeutic target, including in hepatocellular carcinoma (HCC), strategies have been developed on the basis of MYC amplification or gene translocation. Due to the failure of these strategies to provide accurate diagnostics and prognostic value, we have developed a Negative Elongation Factor E (NELFE)-Dependent MYC Target (NDMT) gene signature. This signature, which consists of genes regulated by MYC and NELFE, an RNA binding protein that enhances MYC-induced hepatocarcinogenesis, is predictive of NELFE/MYC-driven tumors that would otherwise not be identified by gene amplification or translocation alone. We demonstrate the utility of the NDMT gene signature to predict a unique subtype of HCC, which is associated with a poor prognosis in three independent cohorts encompassing diverse etiologies, demographics, and viral status. The application of gene signatures, such as the NDMT signature, offers patients access to personalized risk assessments, which may be utilized to direct future care

Knowledge sharing between design and manufacture

Object-oriented modelling has become an established technique for product and manufacturing knowledge representation. Various models offering generalised classes and class hierarchies have been proposed for this purpose. Additional bespoke classes are however typically required for specific domain representations. This causes problems when knowledge needs to be shared between domains using different models to describe common entities. These issues are especially complex when several systems are involved. For example, a designer accessing product, manufacturing, and third party systems may face multiple definitions of components, facilities and processes. This paper proposes a model that addresses some of these issues. The proposed model can describe manufacturing knowledge without additional bespoke classes. The detailed semantics of the model are based on recent work on ontologies, notably the Process Specification Language (PSL). Whilst PSL provides detailed semantics, it is not inherently object-oriented. The integration of PSL with object-oriented modelling methods is therefore the principle contribution of this work

Knowledge reuse in manufacturability analysis

This paper proposes a knowledge representation method that supports greater reuse of manufacturing knowledge in design. The method draws on recent research into object-oriented product and manufacturing models, and problem solving agents. A research platform is proposed, and the results of a test case (based on a simplified jet engine combustion chamber) are described. The paper concludes with three basic principles of reuse, i.e. product/process separation, procedural/declarative knowledge separation, and guidelines for the optimum location of rules and constraints within product/manufacturing models

Whole-Genome Sequencing and Concordance Between Antimicrobial Susceptibility Genotypes and Phenotypes of Bacterial Isolates Associated with Bovine Respiratory Disease.

Extended laboratory culture and antimicrobial susceptibility testing timelines hinder rapid species identification and susceptibility profiling of bacterial pathogens associated with bovine respiratory disease, the most prevalent cause of cattle mortality in the United States. Whole-genome sequencing offers a culture-independent alternative to current bacterial identification methods, but requires a library of bacterial reference genomes for comparison. To contribute new bacterial genome assemblies and evaluate genetic diversity and variation in antimicrobial resistance genotypes, whole-genome sequencing was performed on bovine respiratory disease-associated bacterial isolates (Histophilus somni, Mycoplasma bovis, Mannheimia haemolytica, and Pasteurella multocida) from dairy and beef cattle. One hundred genomically distinct assemblies were added to the NCBI database, doubling the available genomic sequences for these four species. Computer-based methods identified 11 predicted antimicrobial resistance genes in three species, with none being detected in M. bovis While computer-based analysis can identify antibiotic resistance genes within whole-genome sequences (genotype), it may not predict the actual antimicrobial resistance observed in a living organism (phenotype). Antimicrobial susceptibility testing on 64 H. somni, M. haemolytica, and P. multocida isolates had an overall concordance rate between genotype and phenotypic resistance to the associated class of antimicrobials of 72.7% (P < 0.001), showing substantial discordance. Concordance rates varied greatly among different antimicrobial, antibiotic resistance gene, and bacterial species combinations. This suggests that antimicrobial susceptibility phenotypes are needed to complement genomically predicted antibiotic resistance gene genotypes to better understand how the presence of antibiotic resistance genes within a given bacterial species could potentially impact optimal bovine respiratory disease treatment and morbidity/mortality outcomes

The quantization of the chiral Schwinger model based on the BFT-BFV formalism II

We apply an improved version of Batalin-Fradkin-Tyutin (BFT) Hamiltonian method to the a=1 chiral Schwinger Model, which is much more nontrivial than the a>1.$one. Furthermore, through the path integral quantization, we newly resolve the problem of the non-trivial$\delta$function as well as that of the unwanted Fourier parameter$\xi$ in the measure. As a result, we explicitly obtain the fully gauge invariant partition function, which includes a new type of Wess-Zumino (WZ) term irrelevant to the gauge symmetry as well as usual WZ action.Comment: 17 pages, To be published in J. Phys.

The trpE Gene Negatively Regulates Differentiation of Heterocysts at the Level of Induction in Anabaena sp. Strain PCC 7120

Levels of 2-oxoglutarate (2-OG) reflect nitrogen status in many bacteria. In heterocystous cyanobacteria, a spike in the 2-OG level occurs shortly after the removal of combined nitrogen from cultures and is an integral part of the induction of heterocyst differentiation. In this work, deletion of one of the two annotated trpE genes in Anabaena sp. strain PCC 7120 resulted in a spike in the 2-OG level and subsequent differentiation of a wild-type pattern of heterocysts when filaments of the mutant were transferred from growth on ammonia to growth on nitrate. In contrast, 2-OG levels were unaffected in the wild type, which did not differentiate under the same conditions. An inverted-repeat sequence located upstream of trpE bound a central regulator of differentiation, HetR, in vitro and was necessary for HetR-dependent transcription of a reporter fusion and complementation of the mutant phenotype in vivo. Functional complementation of the mutant phenotype with the addition of tryptophan suggested that levels of tryptophan, rather than the demonstrated anthranilate synthase activity of TrpE, mediated the developmental response of the wild type to nitrate. A model is presented for the observed increase in 2-OG in the trpE mutant

Low-pass sequencing for microbial comparative genomics

BACKGROUND: We studied four extremely halophilic archaea by low-pass shotgun sequencing: (1) the metabolically versatile Haloarcula marismortui; (2) the non-pigmented Natrialba asiatica; (3) the psychrophile Halorubrum lacusprofundi and (4) the Dead Sea isolate Halobaculum gomorrense. Approximately one thousand single pass genomic sequences per genome were obtained. The data were analyzed by comparative genomic analyses using the completed Halobacterium sp. NRC-1 genome as a reference. Low-pass shotgun sequencing is a simple, inexpensive, and rapid approach that can readily be performed on any cultured microbe. RESULTS: As expected, the four archaeal halophiles analyzed exhibit both bacterial and eukaryotic characteristics as well as uniquely archaeal traits. All five halophiles exhibit greater than sixty percent GC content and low isoelectric points (pI) for their predicted proteins. Multiple insertion sequence (IS) elements, often involved in genome rearrangements, were identified in H. lacusprofundi and H. marismortui. The core biological functions that govern cellular and genetic mechanisms of H. sp. NRC-1 appear to be conserved in these four other halophiles. Multiple TATA box binding protein (TBP) and transcription factor IIB (TFB) homologs were identified from most of the four shotgunned halophiles. The reconstructed molecular tree of all five halophiles shows a large divergence between these species, but with the closest relationship being between H. sp. NRC-1 and H. lacusprofundi. CONCLUSION: Despite the diverse habitats of these species, all five halophiles share (1) high GC content and (2) low protein isoelectric points, which are characteristics associated with environmental exposure to UV radiation and hypersalinity, respectively. Identification of multiple IS elements in the genome of H. lacusprofundi and H. marismortui suggest that genome structure and dynamic genome reorganization might be similar to that previously observed in the IS-element rich genome of H. sp. NRC-1. Identification of multiple TBP and TFB homologs in these four halophiles are consistent with the hypothesis that different types of complex transcriptional regulation may occur through multiple TBP-TFB combinations in response to rapidly changing environmental conditions. Low-pass shotgun sequence analyses of genomes permit extensive and diverse analyses, and should be generally useful for comparative microbial genomics