Artificial reef monitoring in Florida coastal counties

This bulletin describes artificial reef monitoring programs in Florida. At least half the nation’s human-made marine reefs are estimated to be in Florida waters and high interest exists statewide among fishing, diving, governmental, economic and other stakeholders in the performance of these structures and the habitats that they create. Further, the level and breadth of activity in Florida makes it a bellwether, nationally and worldwide, for interests concerned with aquatic science, ecosystem management and environmental technology for habitat restoration or creation. Recent developments in Florida have prompted increased interest and effort in “monitoring” of reefs. These include extensive studies and disseminating results by the academic research community and the advent of governmental funding for monitoring projects in Florida counties. In response, this study was undertaken to gauge this growing field. Assessment of reef performance—including evaluation of how well they meet objectives for which they were created—has been a neglected subject in many areas of the world. More immediate issues of reef materials selection, siting and deployment usually take precedence in the short-term. A principal readership for this paper is the network of county-level organizations and individuals whose largely independent efforts, taken as a composite, make up much of the Florida reef “program.” Information presented here describes the extent and nature of reef monitoring in Florida counties, how data are managed and aspects of communication about reefs. (16pp.

Florida marine biotechnology: research, development and training capabilities to advance science and commerce

The level of activity and interest in “marine biotechnology” among Florida university faculty and allied laboratory scientists is reported in this document. The information will be used to (1) promote networking and collaboration in research and education, (2) inform industry of possible academic partners, (3) identify contacts interested in potential new sources of funding, and (4) assist development of funding for a statewide marine biotechnology research, training and development program. This document is the first of its kind. Institutions of higher learning were given the opportunity to contribute both an overview of campus capabilities and individual faculty Expressions of Scientific Interest. They are listed in the table of contents. (104pp.

Measurement of residual stresses-- a literature survey

A summary of the different methods for measuring residual stresses is presented. Seventeen of the methods are described and referred to in the discussion of the methods used

Lytic switch protein (ORF50) response element in the Kaposi’s sarcoma-associated herpesvirus K8 promoter is located within but does not require a palindromic structure

Kaposi's sarcoma-associated virus (KSHV) ORF50 protein induces lytic replication and activates the K8 promoter. We show that ORF50-induced and tetradecanoyl phorbol acetate (TPA) induced K8 transcripts initiated from the same start site. A newly identified palindrome (PAL2), containing a 12-bp response region required for ORF50-induced activation in lymphoid cells, was identified in the K8 promoter. Specific DNA binding of bacterially expressed ORF50 was not seen with the K8 promoter despite specific binding to the PAN promoter. The new palindrome shared homology with a previously described ORF50 response element (50RE(K8) and 50RE(57)). We demonstrate that the new 50RE(K8) (50RE(K8-PAL2)) is not the palindrome per se. Instead, the response element is buried within the right arm of the palindrome. We propose that the complexity of the K8 response elements reflects the complexity of mechanisms used by ORF50 during viral reactivation