The effects of verbal descriptions on performance in lineups and showups

Raw data of 5 experiment

Feeling the future of eyewitness research

The authors have no conflicts of interest to disclose. The data were presented at the 2020 meeting of the Psychonomic Society. This study was supported by a grant from Arnold Ventures. The funding source had no role other than financial support.Peer reviewe

Draft genome comparison of representatives of the three dominant genotype groups of dairy Bacillus licheniformis Strains

The spore-forming bacterium Bacillus licheniformis is a common contaminant of milk and milk products. Strains of this species isolated from dairy products can be differentiated into three major groups, namely, G, F1, and F2, using random amplification of polymorphic DNA (RAPD) analysis; however, little is known about the genomic differences between these groups and the identity of the fragments that make up their RAPD profiles. In this work we obtained high-quality draft genomes of representative strains from each of the three RAPD groups (designated strain G-1, strain F1-1, and strain F2-1) and compared them to each other and to B. licheniformis ATCC 14580 and Bacillus subtilis 168. Whole-genome comparison and multilocus sequence typing revealed that strain G-1 contains significant sequence variability and belongs to a lineage distinct from the group F strains. Strain G-1 was found to contain genes coding for a type I restriction modification system, urease production, and bacitracin synthesis, as well as the 8-kbp plasmid pFL7, and these genes were not present in strains F1-1 and F2-1. In agreement with this, all isolates of group G, but no group F isolates, were found to possess urease activity and antimicrobial activity against Micrococcus. Identification of RAPD band sequences revealed that differences in the RAPD profiles were due to differences in gene lengths, 3' ends of predicted primer binding sites, or gene presence or absence. This work provides a greater understanding of the phylogenetic and phenotypic differences observed within the B. licheniformis species

Muscle-Bound Primordial Stem Cells Give Rise to Myofiber-Associated Myogenic and Non-Myogenic Progenitors

Myofiber cultures give rise to myogenic as well as to non-myogenic cells. Whether these myofiber-associated non-myogenic cells develop from resident stem cells that possess mesenchymal plasticity or from other stem cells such as mesenchymal stem cells (MSCs) remain unsolved. To address this question, we applied a method for reconstructing cell lineage trees from somatic mutations to MSCs and myogenic and non-myogenic cells from individual myofibers that were cultured at clonal density

El defensor de Córdoba : diario católico: Año IV Número 781 - 1902 abril 21

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200

Management of the sick child

Of the 9 million deaths/yr that occur in children <5yrs, 3.6 million occur in the 1st month of life and 70% of the others are caused by acute respiratory infections, diarrhoea, measles, and malaria, +/− malnutrition. Most very sick children present with clinical features of more than one diagnosis; ∴ a single diagnosis at admission is often impossible. In many low-resource settings, there may be no paediatrician and sick children are managed by non-specialists. Deaths in hospitals often occur within the first 24h, so sick children need to be identified on arrival, and appropriate treatment instituted. The Emergency Triage Assessment and Treatment (ETAT) strategy, advocated by WHO, is a rapid screening process to identify children who require immediate treatment to avert death and long-term morbidity. Treatment of sick children must never be on a ‘first come, first served’ basis. WHO has published guidelines for the management of common childhood illnesses (second edition 2013), available online (see Box 1.1)</p

Optimizing the selection of fillers in police lineups

A typical police lineup contains a photo of one suspect (who is innocent in a target-absent lineup and guilty in a target-present lineup) plus photos of five or more fillers who are known to be innocent. To create a fair lineup in which the suspect does not stand out, two filler selection methods are commonly used. In the first, fillers are selected if they are similar in appearance to the suspect. In the second, fillers are selected if they possess facial features included in the witness’s description of the culprit (e.g., “20-y-old white male”). The police sometimes use a combination of the two methods by selecting description-matched fillers whose appearance is also similar to that of the suspect in the lineup. Decades of research on which approach is better remains unsettled. Here, we tested a counterintuitive prediction made by a formal model based on signal detection theory: From a pool of acceptable description-matched photos, selecting fillers whose appearance is otherwise dissimilar to the suspect should increase the hit rate without affecting the false-alarm rate (increasing discriminability). In Experiment 1, we confirmed this prediction using a standard mock-crime paradigm. In Experiment 2, the effect on discriminability was reversed (as also predicted by the model) when fillers were matched on similarity to the perpetrator in both target-present and target-absent lineups. These findings suggest that signal-detection theory offers a useful theoretical framework for understanding eyewitness identification decisions made from a police lineup

Hypervariable pili and flagella genes provide suitable new targets for DNA high-resolution melt-based genotyping of dairy Geobacillus spp.

Although nonpathogenic in nature, spores of Geobacillus are able to attach to surfaces, germinate, and form biofilms, allowing rapid multiplication and persistence within milk powder processing plants, causing final product contamination, and eventually leading to a loss of revenue in terms of downgraded product quality. As a result, Geobacillus spp. have been found to be common contaminants of milk powder worldwide. Genotyping methods can help in gaining insight into the ecology and transmission of these thermophilic bacteria within and between dairy processing plants. The objective of this study was to use the assembled draft genomes of two Geobacillus spp. to identify and test new hypervariable genotyping targets for differentiating closely related dairy Geobacillus isolates. The two Geobacillus spp. strains obtained from high spore count powders were obtained in 2010 (isolate 7E) and in 1995 (isolate 126) and were previously shown to be of same genotype based on a variable number tandem repeat genotyping method. Significant nucleotide sequence variation was found in genes encoding pili and flagella, which were further investigated as suitable loci for a new high-resolution melt analysis (HRMA)-based genotyping method. Three genes encoding pulG (containing prepilin-type N-terminal cleavage domain), pilT (pili retraction protein), and fliW (flagellar assembly protein) were selected as targets for the new pili/flagella gene (PilFla) HRMA genotyping method. The threegene- based PilFla-HRMA genotyping method differentiated 35 milk powder Geobacillus spp. isolates into 19 different genotype groups (D =0.93), which compared favorably to the previous method (which used four variable number tandem repeat loci) that generated 16 different genotype groups (D = 0.90). In conclusion, through comparative genomics of two closely related dairy Geobacillus strains, we have identified new hypervariable regions that prove to be useful targets for highly discriminatory genotyping