174 research outputs found
A computational study of magnesium point defects and diffusion in forsterite
We have studied the formation and migration of point defects within the magnesium sub-lattice inforsterite using a combination of empirical and quantum mechanical modelling methodologies. Empiricalmodels based on a parameterised force field coupled to a high throughput grid computing infrastructureallow rapid evaluation of a very large number of possible defect configurations. An embedded clusterapproach reveals more accurate estimates of defect energetics for the most important defect configurations. Considering all defects in their minimum energy, equilibrium positions, we find that the lowest energy intrinsic defect is the magnesium Frenkel type, where a magnesium atom moves from the M1 site to form a split interstitial defect. This defect has 2 four-co-ordinated magnesium atoms located outside opposite triangular faces of an otherwise vacant M1 octahedron. The split interstitial defect is more stable than regular interstitials where magnesium is located in either of the two structurally vacant octahedral sites in the hexagonally close packed oxygen lattice. M1 vacancies are also found to form when iron(II) oxidises to iron(III). The energy of the defects away fromthe equilibrium positions allows the energy barrier to diffusion to be calculated.Wehave considered the migration of both magnesium vacancies and interstitials and find that vacancies are more mobile. When the contribution from the formation energy of the defects is included we arrive at activation energies for vacancy diffusion that are in agreement with experiment
A Consensus Map in Cultivated Hexaploid Oat Reveals Conserved Grass Synteny with Substantial Subgenome Rearrangement
Hexaploid oat ( L., 2 = 6 = 42) is a member of the Poaceae family and has a large genome (∼12.5 Gb) containing 21 chromosome pairs from three ancestral genomes. Physical rearrangements among parental genomes have hindered the development of linkage maps in this species. The objective of this work was to develop a single high-density consensus linkage map that is representative of the majority of commonly grown oat varieties. Data from a cDNA-derived single-nucleotide polymorphism (SNP) array and genotyping-by-sequencing (GBS) were collected from the progeny of 12 biparental recombinant inbred line populations derived from 19 parents representing oat germplasm cultivated primarily in North America. Linkage groups from all mapping populations were compared to identify 21 clusters of conserved collinearity. Linkage groups within each cluster were then merged into 21 consensus chromosomes, generating a framework consensus map of 7202 markers spanning 2843 cM. An additional 9678 markers were placed on this map with a lower degree of certainty. Assignment to physical chromosomes with high confidence was made for nine chromosomes. Comparison of homeologous regions among oat chromosomes and matches to orthologous regions of rice ( L.) reveal that the hexaploid oat genome has been highly rearranged relative to its ancestral diploid genomes as a result of frequent translocations among chromosomes. Heterogeneous chromosome rearrangements among populations were also evident, probably accounting for the failure of some linkage groups to match the consensus. This work contributes to a further understanding of the organization and evolution of hexaploid grass genomes
Embryonic Diapause Is Conserved across Mammals
Embryonic diapause (ED) is a temporary arrest of embryo development and is characterized by delayed implantation in the uterus. ED occurs in blastocysts of less than 2% of mammalian species, including the mouse (Mus musculus). If ED were an evolutionarily conserved phenomenon, then it should be inducible in blastocysts of normally non-diapausing mammals, such as domestic species. To prove this hypothesis, we examined whether blastocysts from domestic sheep (Ovis aries) could enter into diapause following their transfer into mouse uteri in which diapause conditions were induced. Sheep blastocysts entered into diapause, as demonstrated by growth arrest, viability maintenance and their ED-specific pattern of gene expression. Seven days after transfer, diapausing ovine blastocysts were able to resume growth in vitro and, after transfer to surrogate ewe recipients, to develop into normal lambs. The finding that non-diapausing ovine embryos can enter into diapause implies that this phenomenon is phylogenetically conserved and not secondarily acquired by embryos of diapausing species. Our study questions the current model of independent evolution of ED in different mammalian orders
A Consensus Map in Cultivated Hexaploid Oat Reveals Conserved Grass Synteny with Substantial Subgenome Rearrangement
Literature And Integrated Studies : Student Edition Reading, Writing & Grammar Skillbook
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Correction of Purine Nucleoside Phosphorylase Deficiency by Retroviral-Mediated Gene Transfer in Mouse S49 T Cell Lymphoma: A Model for Gene Therapy of T Cell Immunodeficiency
D'Nealian and Zaner-Bloser Manuscript Alphabets and Initial Transition to cursive Handwriting
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